Porcine Cytomegalovirus (PCMV)

Ohlinger, Volker

doi:10.1007/978-1-4613-1587-2_8

Cited by 10 publications

(6 citation statements)

References 21 publications

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“…PCMV is a member of the Betaherpesvirinae. It is found in the respiratory tract of pigs and causes atrophic rhinitis (Ohlinger, 1989). A member of the herpesvirus subfamily Gammaherpesvirinae infecting pigs was not known.…”

Section: Introductionmentioning

confidence: 99%

Detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses.

Ehlers

Ulrich

Goltz

1999

Journal of General Virology

105

102

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Evidence for the existence of porcine gammaherpesviruses was obtained by PCR and sequence analysis. Initially, samples of peripheral blood mononuclear cells (PBMC), spleens, lungs, kidneys and livers of pigs from Germany and Spain were tested with a PCR assay which targets conserved regions of the herpesvirus DNA polymerase gene with degenerate and deoxyinosine-substituted primers. Amplicons of identical sequence were obtained from one spleen and two PBMC samples. This sequence showed a high percentage of identity with the DNA polymerase genes of herpesviruses of the oncogenic subfamily Gammaherpesvirinae. Alignment of amino acid sequences showed the highest identity values with bovine gammaherpesviruses, namely alcelaphine herpesvirus type 1 (68 %), ovine herpesvirus type 2 (68 %) and bovine lymphotropic herpesvirus (67 %). Comparison with pseudorabies virus and porcine cytomegalovirus, which are the only porcine herpesvirus species presently known, showed values of only 41 %. PCR analysis of PBMC (n l 39) and spleen (n l 19) samples from German pigs, using primers specific for the novel sequence, revealed a prevalence of 87 and 95 %, respectively. In this analysis, three out of eight spleen samples from Spanish pigs were also positive. Subsequent sequencing of the amplicons revealed the presence of two closely related gammaherpesvirus sequences, differing from each other by 8 % at the amino acid level. The putative novel porcine herpesviruses, from which these sequences originated, were tentatively designated porcine lymphotropic herpesvirus type 1 and type 2 (PLHV-1 and PLHV-2). When using pig organs for xenotransplantation, the presence of these viruses has to be considered.

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Section: Introductionmentioning

confidence: 99%

Detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses.

Ehlers

Ulrich

Goltz

1999

Journal of General Virology

105

102

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“…Porcine cytomegalovirus (PCMV) causes inclusion body rhinitis in swine upto 10 weeks of age, most severly in swine less than 2 weeks old, and thereafter the infection is subclinical [2,3,6]. PCMV has been thought to be a member of the Betaherpesvirinae, based on its biological properties such as restricted host range and a relatively long reproductive cycle which often results in enlargement of infected cells [8], although there has been no genetic data on PCMV such as genome structure or genes.…”

mentioning

confidence: 99%

Analysis of Porcine Cytomegalovirus DNA Polymerase by Consensus Primer PCR.

Rupasinghe

Tajima

Maeda

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. We used a consensus primer PCR method to amplify a region of herpesviral DNA-directed DNA polymerase gene using degenerate primers for initial characterization of the porcine cytomegalovirus (PCMV) genome. The sequence of the PCR product from PCMV DNA template and its alignment with other herpesvirus DNA polymerase counterparts showed that both conserved amino acid residues and conservative amino acid substitutions are in parallel. Phylogenetic analysis revealed that PCMV should be included in the clade comprising human herpesvirus 6 and 7, rather than human and mouse cytomegaloviruses, in Betaherpesvirus subfamily.-KEY WORDS: betaherpesvirus, consensus primer PCR, porcine cytomegalovirus.

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“…It shares sequence homology with human herpesviruses 6 and 7 and human cytomegalovirus (HCMV) [2-6]. It usually induces silent infection in adult pigs but often a fatal, generalized infection in newborn piglets, with 90% of pigs in the UK being seropositive [3,7].…”

Section: Introductionmentioning

confidence: 99%

“…It usually induces silent infection in adult pigs but often a fatal, generalized infection in newborn piglets, with 90% of pigs in the UK being seropositive [3,7]. PCMV has been known to cross the placenta and infect the fetuses in pregnant sows, leading to fetal death or birth of weak piglets [5,6]. …”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions

Yang

Zhang

Liu

et al. 2012

Virol J

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BackgroundPorcine cytomegalovirus (PCMV) induces silent infection in adult pigs but more frequently causes fatal, generalized infection in newborn piglets. This study aimed to develop a new loop-mediated isothermal amplification (LAMP) method for the sensitive, rapid, and inexpensive detection of PCMV under field conditions.MethodsTissue obtained from nine-week-old PCMV-free Landrace pigs or pig samples from postmortem examinations were analyzed. The samples were found to have clinical signs and lesions consistent with inclusion body rhinitis. Six specific primers were designed by targeting the PCMV DNA polymerase (DPOL) DNA. The LAMP reaction was optimized in a water bath. The sensitivity and specificity of LAMP and polymerase chain reaction (PCR) were compared.ResultsPCMV DNA was amplified at 65°C, and the result could be detected as early as 30 min into the reaction. Positive reactions could be visualized by the naked eye as a color change brought on by the addition of SYBR Green. The sensitivity and specificity of LAMP were found to be similar to those of the PCR.ConclusionsLAMP is a high-throughput technique for the detection of PCMV and has a high specificity, sensitivity and simplicity; these factors make it suitable for detection of PCMV under field conditions.

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Porcine Cytomegalovirus (PCMV)

Cited by 10 publications

References 21 publications

Detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses.

Detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses.

Analysis of Porcine Cytomegalovirus DNA Polymerase by Consensus Primer PCR.

Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions

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