Porcine endothelial grafts could survive for a long term without using systemic immunosuppressors: An investigation of feasibility and efficacy of xeno‐Descemet's stripping automated endothelial keratoplasty from <scp>WZS</scp>‐pig to rhesus monkey

Liu, Yang; Zhang, Yingnan; Liang, Qingfeng; Yan, Chen‐Hua; Wang, Li; Zhang, Jing; Pan, Zhiqiang

doi:10.1111/xen.12433

Cited by 9 publications

(11 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Induction therapy and preventions of AMR seem to be crucial, and to date, induction using CS, basiliximab, IVIG, and rituximab and maintenance with tacrolimus and rituximab are a feasible option for human clinical trials based on pig-to-NHP corneal transplantation data ( Choi et al, 2019b ; Kim et al, 2018a ). Since a recent pig-to-NHP endothelial keratoplasty (EK) study showed efficacy under subconjunctival injection of CSs ( Liu et al, 2019 ), this implies that a regional CSs might be an option for patients with compromised endothelial cells.…”

Section: Immunomodulating Agents In Xenotransplantationmentioning

confidence: 99%

“…Recently, a study on how to produce DMEK graft using porcine cornea has been published ( Liu et al, 2018 ). Regarding Descemet stripping automated endothelial keratoplasty (DSAEK), there was one study reporting long-term survivals of the porcine grafts in NHPs with CS ( Liu et al, 2019 ). However, the graft size was too small (6.0 mm) to apply in human ( Liu et al, 2019 ).…”

Section: Preclinical Efficacy Data Of Porcine Corneal Xenotransplantamentioning

confidence: 99%

“…Regarding Descemet stripping automated endothelial keratoplasty (DSAEK), there was one study reporting long-term survivals of the porcine grafts in NHPs with CS ( Liu et al, 2019 ). However, the graft size was too small (6.0 mm) to apply in human ( Liu et al, 2019 ). EK with porcine corneal graft may be beneficial to overcome xenogeneic rejection.…”

Section: Preclinical Efficacy Data Of Porcine Corneal Xenotransplantamentioning

confidence: 99%

See 2 more Smart Citations

Corneal xenotransplantation: Where are we standing?

Yoon¹,

Choi²,

Kim

2021

Progress in Retinal and Eye Research

View full text Add to dashboard Cite

The search for alternatives to allotransplants is driven by the shortage of corneal donors and is demanding because of the limitations of the alternatives. Indeed, current progress in genetically engineered (GE) pigs, the introduction of gene-editing technology by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9, and advanced immunosuppressants have made xenotransplantation a possible option for a human trial. Porcine corneal xenotransplantation is considered applicable because the eye is regarded as an immune-privileged site. Furthermore, recent non-human primate studies have shown long-term survival of porcine xenotransplants in keratoplasty. Herein, corneal immune privilege is briefly introduced, and xenogeneic reactions are compared with allogeneic reactions in corneal transplantation. This review describes the current knowledge on special issues of xenotransplantation, xenogeneic rejection mechanisms, current immunosuppressive regimens of corneal xenotransplantation, preclinical efficacy and safety data of corneal xenotransplantation, and updates of the regulatory framework to conduct a clinical trial on corneal xenotransplantation. We also discuss barriers that might prevent xenotransplantation from becoming common practice, such as ethical dilemmas, public concerns on xenotransplantation, and the possible risk of xenozoonosis. Given that the legal definition of decellularized porcine cornea (DPC) lies somewhere between a medical device and a xenotransplant, the preclinical efficacy and clinical trial data using DPC are included. The review finally provides perspectives on the current standpoint of corneal xenotransplantation in the fields of regenerative medicine.

show abstract

Section: Immunomodulating Agents In Xenotransplantationmentioning

confidence: 99%

Section: Preclinical Efficacy Data Of Porcine Corneal Xenotransplantamentioning

confidence: 99%

Section: Preclinical Efficacy Data Of Porcine Corneal Xenotransplantamentioning

confidence: 99%

See 1 more Smart Citation

Corneal xenotransplantation: Where are we standing?

Yoon¹,

Choi²,

Kim

2021

Progress in Retinal and Eye Research

View full text Add to dashboard Cite

show abstract

“…Previous studies have demonstrated that humoral immunity and cellular immunity are all involved in xeno-corneal transplantation immune rejection. [48][49][50] Choi and colleagues 48 Our previous studies have demonstrated that shortening the processing periods is a useful way to preserve the ultrastructure and characteristics of stromal fibrils during corneal decellularization, and the over-swelling procedure causes a decline or even loss of corneal transparency. 19,20 Regarding the cornea, the sclera is a dense, opaque tissue that is mainly composed of dense interwoven collagen and elastic fibers.…”

Section: F I G U R Ementioning

confidence: 99%

Scleral defect repair using decellularized porcine sclera in a rabbit model

Wang

Zhao

et al. 2020

Xenotransplantation

View full text Add to dashboard Cite

The sclera is the outermost layer of the ocular wall, and its main role is to protect the eyeball. 1,2 Its surface is covered by conjunctiva and fascia and does not have direct contact with the outside world. In addition, the sclera is mainly composed of dense interwoven collagen fibers and elastic fibers, with few cellular components and blood vessels. 3,4 These histological features indicate that when scleral

show abstract

“…For the transplantation to be successful, the thickness of the isolated material has to be reduced by stripping. Liu et al describe the use of Descemet's stripping automated endothelial keratopathy (DSAEK) to process corneas for xenotransplantation . Their work verifies the utility of this method as superior to other methods without the need of immunosuppressive drugs.…”

Section: Corneal Transplantationmentioning

confidence: 99%