Porcine enteric coronavirus infections in wild boar in Poland – a pilot study

Antas, Marta; Olech, Monika; Szczotka-Bochniarz, Anna

doi:10.2478/jvetres-2021-0041

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…A positive control was used to set the cycle threshold (Ct) for evaluating the test results. A VetMAXTM PEDV/TGEV/ SDCoV Kit was used to detect PEDV infection and exclude a potential PEDV-transmissible gastroenteritis virus (TGEV) and PEDV-porcine deltacoronavirus (PDCoV) co-infection [23].…”

Section: Rna Extraction and Pedv Identificationmentioning

confidence: 99%

Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

2021

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Porcine epidemic diarrhoea (PED) is a highly contagious enteric viral disease of pigs with a high morbidity and mortality rate, which ultimately results in huge economic losses in the pig production sector. The etiological agent of this disease is the porcine epidemic diarrhoea virus (PEDV) which is an enveloped, positive single-stranded RNA virus. The aim of this study was to perform molecular characterization of PEDV to identify the strains circulating in Poland. In this study, 662 faecal samples from 2015 to 2021 were tested with reverse transcription quantitative real-time PCR (RT-qPCR) and the results showed that 3.8% of the tested samples revealed a positive result for PEDV. A phylogenetic analysis of the complete genome and complete S gene sequences showed that Polish PEDV strains belonged to the G1b (S-INDEL) subgroup and were closely related to the European PEDV strains isolated from 2014 to 2019. Furthermore, RDP4 analysis revealed that the Polish PEDV strains harboured a recombinant fragment of ~400 nt in the 5’ end of S gene with PEDV and swine enteric coronavirus (SeCoV) being the major and minor parents, respectively. Antigenic analysis showed that the aa sequences of neutralizing epitopes were conserved among the Polish PEDV strains. Only one strain, #0100/5P, had a unique substitution in the COE epitope. However, Polish PEDV strains showed several substitutions, especially in the COE antigen, as compared to the classical strain CV777. To the best of our knowledge, this is the first report concerning the molecular characterization of porcine epidemic diarrhoea virus strains, as well as the first phylogenetic analysis for PEDV in Poland.

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Section: Rna Extraction and Pedv Identificationmentioning

confidence: 99%

Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

2021

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“…Antas et al collected 157 fecal and blood samples from wild boars in Poland to detect viral genetic material and PEDV-specific antibodies using RT-qPCR and ELISA. The results revealed that while no viral genetic material was found, 3.2% were seropositive [65]. These findings suggest that PEDV may not be fatal to wild boars or cause persistent infection, which increases the risk that the PEDV may infect domestic pigs via wild boars.…”

Section: Pedv Transmissionmentioning

confidence: 88%

“…There have been no reports of a comprehensive epidemiological investigation into PEDV in wild boar populations in China as of yet. Although further studies are needed to confirm whether wild boars serve as a reservoir for the PEDV [65], the risk of PEDV transmission to domestic pigs should not be undervalued.…”

Section: Pedv Transmissionmentioning

confidence: 99%

Porcine Epidemic Diarrhea Virus: Etiology, Epidemiology, Antigenicity, and Control Strategies in China

Lei,

Miao,

et al. 2024

Animals

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Porcine epidemic diarrhea virus (PEDV) is a porcine enteric coronavirus, which is one of the main causative agents of porcine epidemic diarrhea (PED), with 100% morbidity and 80–100% mortality in neonatal piglets. Since 2010, large-scale PED caused by highly pathogenic variants of PEDV has occurred successively in China and other countries in the world, posing a great threat to the global pig industry. It has been demonstrated in many investigations that the classic attenuated vaccine strain, PEDV CV777, is insufficient to fully protect against the PEDV variants. Moreover, the maternally derived antibodies elicited by inactivated vaccines also cannot completely protect piglets from infection. In addition, feedback feeding poses a risk of periodic PEDV recurrence in pig farms, making it challenging to successfully limit the spread of PEDV in China. This review focuses on the etiology, epidemiology, antigenicity, and control strategies of PEDV in China and provides information for the formulation of effective control measures.

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“…A post-outbreak serological survey made in eastern Croatia, revealed antibodies against PEDV in 15.62% of tested pigs, most of them bred on large industrial farms [ 24 ]. In Poland, antibodies against PEDV were detected in 3.2% of tested wild boars [ 25 ]. As for PRRSV, high seroprevalences are also described in other European countries, reaching levels between 20 and 60% [ 2 , 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy

Ferrara

D’Anza

Rossi

et al. 2023

Viruses

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Porcine coronaviruses and reproductive and respiratory syndrome (PRRS) are responsible for severe outbreaks that cause huge economic losses worldwide. In Italy, three coronaviruses have been reported historically: porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV). Although repeated outbreaks have been described, especially in northern Italy, where intensive pig farming is common, there is a worrying lack of information on the spread of these pathogens in Europe. In this work, we determined the seroprevalence of three porcine coronaviruses and PRRSV in the Campania region, southern Italy. A total of 443 samples were tested for the presence of antibodies against porcine coronaviruses and PRRSV using four different commercial ELISAs. Our results indicated that PEDV is the most prevalent among porcine coronaviruses, followed by TGEV, and finally PRCV. PRRSV appeared to be the most prevalent virus (16.7%). For coronaviruses, seroprevalence was higher in pigs raised in intensive farming systems. In terms of distribution, TGEV is more widespread in the province of Avellino, while PEDV and PRRSV are more prevalent in the province of Naples, emphasizing the epidemic nature of both infections. Interestingly, TGEV-positive animals are more common among growers, while seropositivity for PEDV and PRRSV was higher in adults. Our research provides new insights into the spread of swine coronaviruses and PRRSV in southern Italy, as well as a warning about the need for viral surveillance.

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Porcine enteric coronavirus infections in wild boar in Poland – a pilot study

Cited by 8 publications

References 36 publications

Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

Porcine Epidemic Diarrhea Virus: Etiology, Epidemiology, Antigenicity, and Control Strategies in China

A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy

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