Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

Song, Daesub; Park, Bong‐Kyun

doi:10.1007/s11262-012-0713-1

Cited by 571 publications

(652 citation statements)

References 83 publications

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“…Most studies of PED have focused mainly on pathogen isolation and identification, genome and structural protein analysis, diagnosis and vaccine development (Song & Park, 2012); however, the detailed molecular mechanism of viral pathogenesis remains unknown. The outcomes of viral infection are primarily determined by the interplay between the virus and the innate immune system (Frieman et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Porcine epidemic diarrhea virus infection induces NF-κB activation through the TLR2, TLR3 and TLR9 pathways in porcine intestinal epithelial cells

Cao

Gao

et al. 2015

Journal of General Virology

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Porcine epidemic diarrhea virus (PEDV) is a coronavirus that induces persistent diarrhoea in swine, resulting in severe economic losses in swine-producing countries. Insights into the interplay between PEDV infection and the innate immune system are necessary for understanding the associated mechanism of pathogenesis. The transcription factor NF-kB plays an important role in regulating host immune responses. Here, we elucidated for the first time to our knowledge the potential mechanism of PEDV-mediated NF-kB activation in porcine small intestinal epithelial cells (IECs). During PEDV infection, NF-kB p65 was found to translocate from the cytoplasm to the nucleus, and PEDV-dependent NF-kB activity was associated with viral dose and active replication. Using small interfering RNAs to screen different mRNA components of the Toll-like receptor (TLR) or RIG-I-like receptor signalling pathways, we demonstrated that TLR2, TLR3 and TLR9 contribute to NF-kB activation in response to PEDV infection, but not RIG-I. By screening PEDV structural proteins for their ability to induce NF-kB activities, we found that PEDV nucleocapsid protein (N) could activate NF-kB and that the central region of N was essential for NF-kB activation. Furthermore, TLR2 was involved in PEDV N-induced NF-kB activation in IECs. Collectively, these findings provide new avenues of investigation into the molecular mechanisms of NF-kB activation induced by PEDV infection.

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Section: Introductionmentioning

confidence: 99%

Porcine epidemic diarrhea virus infection induces NF-κB activation through the TLR2, TLR3 and TLR9 pathways in porcine intestinal epithelial cells

Cao

Gao

et al. 2015

Journal of General Virology

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“…All strains were obtained from farms that used the CV777-based inactivated vaccine but had 100% prevalence of diarrhea in pigs (Technical Appendix Table 1). Another 2 fi eld isolates (CH3, CH4) from 2 farms with pigs with severe diarrhea shared the highest sequence identity with attenuated strain DR13 from South Korea (99.2% and 99.1%, respectively), which has been in routine use as an oral vaccine against PEDV in South Korea since 2004 (15). The appearance of strains in China similar to those from South Korea and their role in the recent PEDV outbreak should be further investigated.…”

mentioning

confidence: 99%

New Variants of Porcine Epidemic Diarrhea Virus, China, 2011

Li¹,

Li²,

Liu³

et al. 2012

Emerg. Infect. Dis.

367

229

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“…Subsequently, it was found in Belgium, Hungary, France, Italy, the Czech Republic, China and Asia, where more severe outbreaks were diagnosed. In the USA, it was first diagnosed in May 2013, and during the period from September 2013 to February 2014, losses of pigs in the United States by the swine epidemic diarrhea virus were estimated in 2.7 million or slightly more than 5% of the animals [123][124][125][126]. Recently, the presence of the virus has been reported in countries of South America, such as Peru [127] and Colombia [128].…”

Section: Epidemic Porcine Diarrhea (Pedv)mentioning

confidence: 99%

Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

Catroxo¹,

Martins²

2018

Stomach Disorders

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Gastroenteritis caused by viruses is considered to be one of the most important diseases in livestock, being the main cause of morbidity and mortality in young animals, culminating in serious economic losses due to costs with prophylaxis and treatment, increased susceptibility of animals to secondary infections, developmental delay and death. Stressful factors may support the onset of illness. Several viral agents can cause gastroenteritis in various animal species. Rotaviruses are considered the main cause of enteric infections in various animals, including humans constituting important zoonosis. Due to genetic diversity and their ability to cross the species barrier, the coronaviruses infect many species. In cattle, they cause "Winter Dysentery" in adult animals and "Neonatal Diarrhea" in newborn calves. In swine, they are responsible for "Transmissible Gastroenteritis" and "Swine Epidemic Diarrhea." Equines infected with coronavirus also develop severe gastroenteritis. Bovine viral diarrhea (BVD) caused by a flavivirus of the genus Pestivirus is related to digestive and reproductive disorders, affecting any productive sector, are it cut, milk or confinement. Transmission electron microscopy is an indispensable tool in the diagnosis of viral gastroenteric infectious diseases. Negative staining is a simple, fast and efficient technique, being ideal for the detection of gastroenteric viruses, being easily visualized. The immunoelectron microscopy (IEM) technique allows increasing the sensitivity of virus detection where low concentrations of virus are aggregated so that they may be more easily seen. The immunolabeling with colloidal gold technique utilizes specific antibodies tagged with particles of colloidal gold to label the antigen antibody reaction. Embedding resin technique allows obtaining information on the virus-cell interaction. The different transmission electron microscopy modalities promotes a fast and accurate diagnosis of the different gastroenteric viral agents, allowing prophylactic measures of control and prevention in the creations to be promptly instituted, avoiding animal losses and disastrous economic losses, and collaborating with the National Porcine and Bovine Agribusiness.

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Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines

Cited by 571 publications

References 83 publications

Porcine epidemic diarrhea virus infection induces NF-κB activation through the TLR2, TLR3 and TLR9 pathways in porcine intestinal epithelial cells

Porcine epidemic diarrhea virus infection induces NF-κB activation through the TLR2, TLR3 and TLR9 pathways in porcine intestinal epithelial cells

New Variants of Porcine Epidemic Diarrhea Virus, China, 2011

Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

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