Porcine epidemic diarrhoea virus with a recombinant S gene detected in Hungary, 2016

Valkó, Anna; Biksi, Imre; Cságola, Attila; Tuboly, Tamás; Kiss, Krisztián; Ursu, Krisztina; Dán, Ádám

doi:10.1556/004.2017.025

Cited by 18 publications

(29 citation statements)

References 29 publications

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“…Surprisingly, PEDV was not found in any samples, as opposed to what has been assumed after the reappearance of the virus in the country in 2016 (Valkó et al, 2017). The case was similar with TGEV, which was detected for the last time in Hungary in 2013 (Lőrincz et al, 2014), although the absence of this coronavirus may be explained by the cross-protection provided by antibodies produced against PRCV.…”

Section: Discussionmentioning

confidence: 54%

“…All viruses mentioned above may contribute to the development of diarrhoea and might be present in the animal at the same time, since enteric disease of swine can be a complex disorder involving multiple microorganisms with a dominance of viruses (Katsuda et al, 2006). After the reappearance of PEDV in 2016 in Hungary (Valkó et al, 2017), an extensive survey was initiated to determine the frequency of PEDV and other diarrhoea-associated viruses. Our aim was to assess the occurrence of such viruses in faecal samples of healthy and di-Acta Veterinaria Hungarica 67, 2019 arrhoeic pigs of various ages by PCR and conduct a comprehensive analysis about their presence on Hungarian pig farms for the first time in the country.…”

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confidence: 99%

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Frequency of diarrhoea-associated viruses in swine of various ages in Hungary

Valkó

Marosi

Cságola

et al. 2019

Acta Veterinaria Hungarica

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Enteric viral diseases of swine are one of the most frequent disorders causing huge economic losses in pork production. After the reappearance of an emerging enteropathogen, porcine epidemic diarrhoea virus (PEDV) in Hungary in 2016, an extensive survey was initiated in an attempt to identify diarrhoea-related porcine viruses, including adeno-, astro-, boca-, calici-, circo-, corona-, kobu-, rota-and Torque teno viruses. A total of 384 faecal samples collected during a twoyear period from diarrhoeic and asymptomatic pigs of various ages in 17 farms were screened by conventional and real-time PCR methods. Half of the samples contained at least one examined virus with the dominance of kobuvirus (55.1%) followed by bocaviruses (33.2%) and rotavirus groups A and C together (20.9%), while coronaviruses including PEDV were not found in this set of samples. Statistical analysis showed a highly significant difference (P < 0.0001) in the frequency of single infections compared to mixed ones with the exception of weaned pigs, in which group additionally most viruses were detected. The results of this study suggest that the complexity of this disease may vary with age, which makes the prevention of diarrhoea a challenge, especially in weaned pigs.

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Section: Discussionmentioning

confidence: 54%

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confidence: 99%

Frequency of diarrhoea-associated viruses in swine of various ages in Hungary

Valkó

Marosi

Cságola

et al. 2019

Acta Veterinaria Hungarica

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“…In total, 360 of 491 (73.3%) showed a high degree of similarity (97.6%–100%) with the European S‐INDEL strains circulating in 2014–2015 (Figure ). The other 131 sequences (26.7%) exhibited a high degree of identity (98.6%–99.8%) with the PEDV SLOreBAS‐1 strain identified in Slovenia in 2015 () and in Hungary in 2016 (Valko et al., ). This strain harbours a recombinant S1 gene containing a fragment of ~400 nt that has a high identity with SeCoV, an already recombinant strain.…”

Section: Resultsmentioning

confidence: 92%

Porcine Epidemic Diarrhoea Virus in Italy: Disease spread and the role of transportation

Boniotti

Papetti

Bertasio

et al. 2018

Transbound Emerg Dis

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Porcine Epidemic Diarrhoea Virus (PEDV) causes watery diarrhoea, dehydration, and a high mortality rate among suckling pigs. Recently, PEDV had a large negative economic impact on the swine industries in Asia and North America. In 2014, PEDV re-emerged in many European countries, but most countries only reported a few sporadic cases. Here, we report the epidemic wave that occurred in Italy from 2015 to 2017. During this time, PEDV was detected by real-time PCR in 438 farms located mainly in the high-density pig production area in Northern Italy. Most of the outbreaks were in farrow-to-finish, farrow-to-wean and finisher farms. Clinical signs were observed mainly in suckling and fattening animals, while mortality rates were higher in piglets, reaching 50%. A sequence analysis showed that a PEDV strain, similar to the OH851 S-INDEL strain isolated in the USA in January 2014, was responsible for the outbreaks in Italy in 2015 and 2016. However, from January 2017, a recombinant variant strain, containing a portion of the Swine Enteric Coronavirus in the S1 gene, spread and almost completely outcompeted the previous nonrecombinant strain. In total, 14.1% of the environmental swabs collected from trucks at slaughterhouses after animals were unloaded tested positive for PEDV before the trucks were cleaned and disinfected, and 46% remained positive after cleaning and disinfection processes were performed. Moreover, environmental swabs indicated that 17.3% of the empty trucks arriving at the farms to load animals were PEDV-positive. This study indicates that trucks can have an important role in the spread of PEDV in Italy.

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“…Beyond China, the disease has rapidly spread to more than 38 states in the USA following its first outbreak in May 2013, affecting more than 4,000 farms accounting for more than 7 million piglets (Cima, 2014). Japan, Canada, Mexico, and Colombia have also experienced successive outbreaks, with considerable economic losses to the global pig industry (Lara-Romero et al, 2018;Ojkic et al, 2015;Takahashi, Okada, & Ohshima, 1983;Valko et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains

Guo

Fang

et al. 2018

Transbound Emerg Dis

103

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Porcine epidemic diarrhea virus (PEDV), which re-emerged in China in October 2010, has spread rapidly worldwide. Detailed analyses of the complete genomes of different PEDV strains are essential to understand the relationships among re-emerging and historic strains worldwide. Here, we analysed the complete genomes of 409 strains from different countries, which were classified into five subgroup strains (i.e., GI-a, GI-b, GII-a, GII-b, and GII-c). Phylogenetic study of different genes in the PEDV strains revealed that the newly discovered subgroup GII-c exhibited inconsistent topologies between the spike gene and other genes. Furthermore, recombination analysis indicated that GII-c viruses evolved from a recombinant virus that acquired the 5' part of the spike gene from the GI-a subgroup and the remaining genomic regions from the GII-a subgroup. Molecular clock analysis showed that divergence of the GII-c subgroup spike gene occurred in April 2010, suggesting that the subgroup originated from recombination events before the PEDV re-emergence outbreaks. Interestingly, Ascaris suum, a large roundworm occurring in pigs, was found to be an unusual PEDV host, providing potential support for cross-host transmission. This study has significant implications for understanding ongoing global PEDV outbreaks and will guide future efforts to develop effective preventative measures against PEDV.

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Porcine epidemic diarrhoea virus with a recombinant S gene detected in Hungary, 2016

Cited by 18 publications

References 29 publications

Frequency of diarrhoea-associated viruses in swine of various ages in Hungary

Frequency of diarrhoea-associated viruses in swine of various ages in Hungary

Porcine Epidemic Diarrhoea Virus in Italy: Disease spread and the role of transportation

Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains

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