Porcine eye lens crystallins: antigenic similarity with human crystallins and tool for the detection of anti-crystallin antibodies

Trifonova, N.; Kalaydjiev, S.; Stamenova, Maria; Trifonova, Radiana; Breipohl, W.

doi:10.1007/s00417-002-0495-6

Cited by 8 publications

(4 citation statements)

References 10 publications

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“…This similarity was demonstrated by Trifonova et al [23], where they used watersoluble extracts of human and porcine lenses. Indirect ELISA of both antigens showed antigenic identity, which makes the porcine crystallins an adequate substitute for human crystallins.…”

Section: Resultsmentioning

confidence: 60%

Serum anti-α-crystallin antibodies in women with endocrine disorders

Buteva-Hristova¹,

Lazarov

Lozanov

et al. 2017

Biotechnology & Biotechnological Equipment

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There is a distinct group among the patients with unexplained infertility who are found to have enhanced humoral immune response. Recent studies focus on the expression of stress proteins being an important factor in the stages of gametogenesis, fertilization, implantation, early embryonic development and pregnancy. Increased expression of stress proteins and immune response against them was found in tissues exposed to stress. There is not enough data linking infertility to expression and immunity of a-crystallins in patients with endocrine diseases. The aim of this work was to study anti-a-crystallin antibodies in patients with polycystic ovary syndrome (PCOS), Graves' disease, autoimmune thyroiditis, diabetes mellitus and obesity. Sera samples from 169 women with endocrine disorders (PCOS (n = 68); Graves' disease (n = 26); autoimmune thyroiditis (n = 32); diabetes mellitus (n = 10); and obesity (n = 33)) were tested by ELISA. The statistical analysis was performed using SPSS program. The concentration of anti-a-crystallin antibodies is significantly elevated in the PCOS group compared to the control group (p = 0.021). The frequency of positive sera in the same group of patients is significantly higher compared to the control group (p = 0.029). In all other groups, no statistically significant elevation was observed. Elevated concentrations of anti-a-crystallin antibodies found in patients with PCOS suggest that the increased production of anti-a-crystallin antibodies in women with PCOS is most probably caused by failure of the immune tolerance and the induction of immune response. This is most likely due to an increased expression of this stress protein as a result of oxidative stress and chronic inflammation.

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Section: Resultsmentioning

confidence: 60%

Serum anti-α-crystallin antibodies in women with endocrine disorders

Buteva-Hristova¹,

Lazarov

Lozanov

et al. 2017

Biotechnology & Biotechnological Equipment

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“…As an antigen we used α-crystallin isolated by gel chromatography of porcine eye lens extract 16. Polyacrylamide gel electrophoresis (PAGE; 12% running gel) was used in order to demonstrate the purity of the α-crystallin fraction.…”

Section: Methodsmentioning

confidence: 99%

The role of small heat-shock protein αB-crystalline (HspB5) in COPD pathogenesis

Cherneva¹,

Georgiev²,

Петрова³

et al. 2012

COPD

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BackgroundαB-crystallin (HspB5) is a chaperone whose role as a marker of innate immunity activation as well as its therapeutic potential have recently been investigated in several inflammatory diseases: multiple sclerosis, myocardial ischemia, and Guillain–Barré syndrome.AimThe aim of this study is to determine the role of αB-crystallin in chronic obstructive pulmonary disease (COPD) pathogenesis and inflammation.MaterialsPlasma levels of αB-crystallin were studied in 163 patients: 52 healthy non-COPD smokers; 20 COPD smokers in Global Initiative for Chronic Obstructive Lung Disease (GOLD) stages I–II; 43 COPD smokers in GOLD stages III-IV. Forty-eight patients were diagnosed with acute inflammatory respiratory disease. The plasma levels of αB-crystallin antibodies were determined by an enzyme-linked immunosorbent assay (Calbiochem), and were confirmed with Western blotting. Tissue expression of the protein was compared in three different groups of patients: COPD smokers, COPD nonsmokers, and in patients with age-related emphysema.ResultsThe mean level of anti-αB-crystallin antibodies in non-COPD smokers was 0.291nm. In COPD smokers it was 0.352 nm and, in patients with inflammatory lung diseases, 0.433 nm. There was a statistically significant difference between COPD smokers and healthy non-COPD smokers (P = 0.010). The same could be observed comparing the group of patients with acute inflammation and non-COPD healthy smokers (P = 0.007). There was no statistically significant difference between patients with mild/moderate inflammation and those with severe COPD. Tissue detection of the protein showed that it was significantly overexpressed in COPD smokers in comparison to COPD nonsmokers and was only slightly expressed in patients with age-related emphysema.ConclusionαB-crystallin is increased in patients with inflammatory lung diseases. Though unspecific, it could be used in a panel of markers discerning COPD smokers from healthy nonsmokers. As αB-crystallin is a regulator of innate immunity and a therapeutic anti-inflammatory agent, its exact role in COPD pathogenesis and therapy should be explored further.

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“…Approximately 30 lenses were needed to generate a single sample. Porcine lenses and their components have been shown to be very similar to human lenses in terms of molecular, immunological, optical, and biometric properties – and, thus, were adopted as an animal model. All analyses were performed within 36 h of animal death.…”

Section: Methodsmentioning

confidence: 99%

Material Characterization of Porcine Lenticular Soluble Proteins

Reilly

Hamilton

et al. 2008

Biomacromolecules

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The soluble proteins present in the ocular lens impart important optical and dynamic mechanical properties on the lens. The short-range order of crystallin proteins grants transparency to a very concentrated protein solution. This unique protein system directly enables proper visual function of the eye. These proteins were investigated in steady and oscillatory shear. Steady shear data were fitted with a modified Herschel-Bulkley yield stress model that allows for a Newtonian plateau at low shear rates. The Cox-Merz rule was used in conjunction with large amplitude oscillatory shear to give insight into the degradation of the fluid structure with increasing strain. The shear thinning viscoelastic behavior of these proteins gives rise to beneficial mechanical properties and results from the same short-range order granting optical transparency.

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Porcine eye lens crystallins: antigenic similarity with human crystallins and tool for the detection of anti-crystallin antibodies

Abstract: These data demonstrated a strong antigenic similarity between human and porcine lens crystallins, suggesting the appropriateness of the use of porcine lens extracts for the detection of humoral anti-lens autoimmune response in patients with eye diseases.

Cited by 8 publications

References 10 publications

Serum anti-α-crystallin antibodies in women with endocrine disorders

Serum anti-α-crystallin antibodies in women with endocrine disorders

The role of small heat-shock protein αB-crystalline (HspB5) in COPD pathogenesis

Material Characterization of Porcine Lenticular Soluble Proteins

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Porcine eye lens crystallins: antigenic similarity with human crystallins and tool for the detection of anti-crystallin antibodies

Abstract: These data demonstrated a strong antigenic similarity between human and porcine lens crystallins, suggesting the appropriateness of the use of porcine lens extracts for the detection of humoral anti-lens autoimmune response in patients with eye diseases.

Cited by 8 publications

References 10 publications

Serum anti-α-crystallin antibodies in women with endocrine disorders

Serum anti-α-crystallin antibodies in women with endocrine disorders

The role of small heat-shock protein &alpha;B-crystalline (HspB5) in COPD pathogenesis

Material Characterization of Porcine Lenticular Soluble Proteins

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The role of small heat-shock protein αB-crystalline (HspB5) in COPD pathogenesis