Porcine Kidney Cell Line Persistently Contaminated with Avirulent Swine Fever Virus

Shimizu, Yoshihisa; Furuuchi, Susumu; Hayashi, S.; Kumagai, Tomohisa; Sasahara, J

doi:10.1099/0022-1317-4-4-625

Cited by 9 publications

(5 citation statements)

References 1 publication

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“…Inoculation of cell culture with equal amounts of pestivirus CSFV/Eand /E + leads to CPE exhibition after NDV challenge (Shimizu et al, 1969), implying a potential inherent balanced strain activity between /E + and /E - (Inaba et al, 1963). However, this does not appear to be the case in our study; instead, this process is dominated by the Evirus, with minor influence from BVDV/E + virus, in MDBK-SY cells.…”

Section: Resultscontrasting

confidence: 64%

Quantitative and rapid interference of bovine viral diarrhea virus BVDV/END– and BVDV/END+ strains

Muhsen¹,

Lee²

2018

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Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) strains. Simultaneous inoculation of MDBK-SY cell monolayers with BVDV/E- virus and a three log higher amount of BVDV RK13/E+ virus, leads to acquisition of the BVDV/E- feature of blocking Newcastle disease virus (NDV) infection in cells. BVDV/E- strains, particularly at a high titre and MOI ≥1.25, can exert and impose their effects in BVDV/E+ infected cells; however, if BVDV/E- MOI is reduced to MOI below 0.625, the BVDV/E+ effect can be restored leading to cytopathic effects (CPE) induction by NDV reciprocal to the titre of the BVDV RK13/E+ strain. Moreover, blocking and prevention of induced CPE by NDV or vesicular stomatitis virus (VSV) occurs even when BVDV/E- superinfects primary BVDV/E+ infected cells, indicating a defective homologous interference between BVDV/E+ and BVDV/E- strains. Taken together, BVDV/E- strains have a strong competitive potency and mediate a fast acting (i.e. within 60 min) influence against BVDV/E+ activity. This may be relevant in vivo where BVDV/E- and BVDV/E+ combinations are frequently isolated from infected individuals.

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Section: Resultscontrasting

confidence: 64%

Quantitative and rapid interference of bovine viral diarrhea virus BVDV/END– and BVDV/END+ strains

Muhsen¹,

Lee²

2018

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“…Most of the detailed comparative studies with NBV and the mammalian reoviruses were carried out in a stable line of pig kidney cells which possibly carry an enterovirus (17). However, the syncytia seen after inoculation of PS cells with NBV were similar to those formed in other stable cell lines and in primary MK and CEF cells.…”

Section: Discussionmentioning

confidence: 99%

Structure and Cytopathic Effects of Nelson Bay Virus

Gard

Compans

1970

J Virol

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A virus isolated from a flying fox (Pteropus poliocephalus) has a morphology similar to that of reoviruses. However, unlike the reoviruses, this virus has a rapid cytopathic effect, causing cell fusion, vacuolization of the cytoplasm, and an unusual nuclear degeneration. Immunofluorescence indicates that viral antigen is distributed through both nucleus and cytoplasm. Viral maturation appears to take

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“…An outbreak in Australia would have significant animal health and economic consequences 19 . CSFV is known for persistent cell line contamination, specifically of porcine cell lines 17,20,21 . The source of CSFV cell contamination is most likely through the use of infected source material 20 .…”

Section: Adventitious Agentsmentioning

confidence: 99%

Imported biologicals: unforeseen biosecurity risks in the laboratory

2020

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Imported biological products are ubiquitous necessities of modern life that can pose significant biosecurity risks to Australia. Products produced using animal material are used everywhere from enzymes in cleaning products, to cell lines and bacterial cultures used to produce vaccines and medicines. This article highlights adventitious agents of biologicals and provides an overview of the considerations and regulatory tools administered under the Biosecurity Act 2015 (Commonwealth) to manage these biosecurity risks whilst still facilitating imports of biologicals.

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Porcine Kidney Cell Line Persistently Contaminated with Avirulent Swine Fever Virus

Cited by 9 publications

References 1 publication

Quantitative and rapid interference of bovine viral diarrhea virus BVDV/END– and BVDV/END+ strains

Quantitative and rapid interference of bovine viral diarrhea virus BVDV/END– and BVDV/END+ strains

Structure and Cytopathic Effects of Nelson Bay Virus

Imported biologicals: unforeseen biosecurity risks in the laboratory

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