Porcine linkage and cytogenetic maps integrated by regional mapping of 100 microsatellites on somatic cell hybrid panel

Robic, Annie; Riquet, Juliette; Yerle, Martine M.; Milan, Denis; Lahbib-Mansais, Y.; Dubut-Fontana, C.; Gellin, Joël

doi:10.1007/s003359900129

Cited by 61 publications

(37 citation statements)

References 21 publications

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“…Twenty-five nanograms of DNA from each hybrid was used. Regional gene assignments were achieved through the concordant segregation of pig gene-specific PCR amplifications and chromosome fragments retained in the hybrid cells as described by Robic et al (1996). The statistical evaluation of data followed Chevalet et al (1997; see http://www.toulouse.inra.fr/lgc/lgc.htlm).…”

Section: Sch Mappingmentioning

confidence: 99%

Xenoduplex Analysis—A Method for Comparative Gene Mapping Using Hybrid Panels

Marklund¹,

Jeon²,

Andersson³

1998

Genome Res.

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Somatic cell hybrid (SCH) panels and radiation hybrid (RH) panels are powerful resources for comparative gene mapping because gene assignments are made without the detection of genetic polymorphism as needed for linkage mapping. A frequently encountered problem, however, is that the gene specific primers may amplify homologous PCR products of equal length from the donor and recipient species of the panel. Here, we describe a simple solution to this problem in which we utilize the formation of interspecies heteroduplexes that can be easily distinguished from the corresponding homoduplexes by native polyacrylamide gel electrophoresis. We denote these DNA–DNA interspecies hybrids, xenoduplexes (xeno = Gr. Xenos, foreigner). A merit of the method is that the formation of xenoduplexes strongly suggests that the PCR products from the two species represent homologous sequences. The method is thus particularly useful for comparative gene mapping when the PCR primers have been designed by use of sequence information from other species. In this study we have successfully used xenoduplex analysis and a pig-rodent SCH panel to map seven porcine genes (ACADM, AT3, HOXD, IL8RB, LEPR, PAX8, PKLR) for which no previous sequence information was available. The assignment of the leptin receptor gene (LEPR) to pig chromosome 6q32–35 excluded LEPR as a candidate gene for a QTL on pig chromosome 4 with a major effect on fatness.

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Section: Sch Mappingmentioning

confidence: 99%

Xenoduplex Analysis—A Method for Comparative Gene Mapping Using Hybrid Panels

Marklund¹,

Jeon²,

Andersson³

1998

Genome Res.

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“…The absence of a loop can be explained by the small size of the inverted chromosome segment (Trunca and Opitz, 1977;Daniel, 1981;Kaiser, 1984), it is physically impossible to form a loop; this was the case for the inversion described by Switonski (1991). It can also be explained by the localization of the breakpoints (Ashley, 1988 (1996) and Robic et al (1996), and one cosmid, BHT12 (Hoyheim et al, 1994). The cosmid hybridizes to the extremity of the normal chromosome 4 short arm (band 4p1.5).…”

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confidence: 99%

A pericentric inversion of chromosome 4 in pigs

Ducos¹,

Pinton²,

Séguéla³

et al. 1997

Genet. Sel. Evol.

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Summary -The main French porcine artificial insemination centre recently decided to carry out a systematic chromosomal evaluation of the animals used for pure-breeding purposes. This practice has allowed the identification of a new pericentric inversion affecting chromosome 4, in four Large White boars originating from four different herds. The cytogenetic evaluation by means of the GTG-banding technique revealed an inversion of the pericentric part of the chromosome, after double breaks, the first one in the 4q2.3 pale band of the long arm, and the second one in the upper border of the 4pl.4 dark band of the short arm. The chromosomal rearrangement could be described, according to the standard nomenclature, as 38,XY,inv(4)(p14q23). This interpretation has been confirmed

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“…The porcine somatic cell hybrid panel used consisted of 27 pig-rodent hybrids (including 19 pig-hamster and 8 pig-mouse hybrids) Robic et al, 1996). For genotyping of the hybrid panel, 25 ng of each cell line DNA and of each control DNA from the parental cells (hamster and mouse) were amplified with the pig MCP primers.…”

Section: Methodsmentioning

confidence: 99%