Porcine placental immunoexpression of vascular endothelial growth factor, placenta growth factor, Flt-1 and Flk-1

Sanchis, EG; Cristofolini, Andrea; Merkis, Cecilia Inés

doi:10.3109/10520295.2015.1019927

Cited by 7 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We investigated the expression profiles of the angiogenic factors, VEGF and PlGF, and their receptors, Flt‐1 and Flk‐1. We demonstrated the localization of both VEGF and PlGF and their receptors during several gestational periods (Sanchis et al., ).…”

Section: Introductionmentioning

confidence: 63%

“…Our finding previously indicates that during the gestational development in the gilts, the nutritional demands increase due to continuous growth of the concepti . The placenta undergoes physiological remodelling processes necessary to regulate the foetal/maternal exchange (Cristofolini, Merkis et al., ; Cristofolini, Sanchis, et al., ; Cristofolini et al., ; Merkis, Cristofolini, & Koncurat, ; Merkis et al., , ; Sanchis et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…We demonstrated the localization of both VEGF and PlGF during several gestational periods and localized Flt‐1 and Flk‐1 receptors at Day 30 and Day 114 of pregnancy. We determined during advanced gestations that angiogenesis is due to angiogenic molecules other than members of VEGF family (Sanchis et al., ). In the present report, the formation of new blood vessels at Day 90 would occur through angiogenic pathways stimulated by other members of VEGF family.…”

Section: Discussionmentioning

confidence: 99%

“…The placenta is a highly vascularized organ (Burton, Charnock‐Jones, & Jauniaux, ; Wooding & Burton, ) that transports substances between foetal and maternal circulations (Barrio et al., ; Greenwood, Slepetis, & Bell, ; Mayhew, ). In every mammalian placenta, extensive foetal and maternal capillary networks develop parallel to their interface to maximize the efficiency of haemotrophic exchange (Abd‐Elnaeim, Leiser, Wilsher, & Allen, ; Sanchis, Cristofolini, & Merkis, ) by minimizing the diffusion distance for gases and solutes (Cristofolini, Merkis, & Koncurat, ; Sanchis, Cristofolini, & Merkis, ). We established earlier the importance of extracellular matrix and apoptosis for the formation, development and support of the placental vascular system (Cristofolini, ; Merkis et al., ; Sanchis, Cristofolini, Taglialegna, & Merkis, ; Cristofolini, Sanchis et al., ; Sanchis et al., ; Cristofolini et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…To achieve a successful pregnancy and a normal gestation, a placental vascularization a correct one must occur allowing a suitable establishment and development of the foetus/embryos (Cristofolini, Merkis et al., ; Sanchis et al., ). Several angiogenic and non‐angiogenic factors are involved in placental vascular development.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Morphometric study of the porcine placental vascularization

Cristofolini

Fiorimanti

Campos

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

The early development in mammals is characterized by the contribution of nutrients from the maternal tissues through the placenta, which is in apposition with foetal membranes and the endometrium, allowing the physiological interchange between the embryos/foetuses and the mother. The aim of this work was to study the number of placental blood vessels and their vascular area through morphometric analyses and the haemotrophic diffusion distance in porcine placental tissues from early gestations, intermediates gestations, advanced gestations and term gestations. For those purposes, morphometric measurements, blood vessel quantification, high-resolution light microscopy and transmission electron microscopy were performed. The implementation of the high-resolution light microscopy allowed studying the placental vascular and tissue histoarchitecture with higher definition and resolution than using a conventional light microscopy. We highlight the close location of the subepithelial capillaries to the maternal/foetal interface as pregnancy progresses. We found statistically significant evidence to state that the area of blood vessels is dependent on the gestation period. In advanced gestations, the presence of numerous small blood vessels and its near location to foetal/maternal interface agree with the great remodelling reported in our previous studies. In conclusion, in gilts, given the type of non-invasive epithelial placentation, the new blood vessels generation and of haemotrophic diffusion distance reduction, determined in this report, assure the maternal/foetal haemotrophic exchange efficiency during gestation.

show abstract

Section: Introductionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Morphometric study of the porcine placental vascularization

Cristofolini

Fiorimanti

Campos

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

Apoptosis and cell proliferation in porcine placental vascularization

Sanchis

Cristofolini

Fiorimanti

et al. 2017

Animal Reproduction Science

View full text Add to dashboard Cite

The placenta is a highly vascularized organ, indispensable tothe transfer of nutrients to the growing fetuses. During gestation, there exists an expansion of the placental vascular network through active angiogenesis. The aim of this research was to study cell proliferation and apoptosis through high resolution light microscopy (HRLM) and transmission electron microscopy (TEM) ultrastructure, immunohistochemistry for Ki67and caspase-3, determination of placental vascular area,and TUNEL assay. Crossbred sows placental tissues from approximately 30±2(n=5), 40±2(n=5), 60±2 (n=5), 80±2(n=5), 90±2(n=5) and 114±2(n=5) days of gestation were used. The evaluation of cell proliferation showed the highest%Ki67 values on days 30±2 and 80±2 of pregnancy. Caspase-3 expressed the highest value on day 30±2, while the highest apoptotic indexes were found on days30±2 and 90±2. The placental vascular area was higher on day 80±2 of pregnancy. According to our results, an active vascular cell remodeling by a caspase-3 dependent apoptosis seems to be present in early pregnancy. The increase in the vascular area on day 80±2 would be the result of the intense vascular cell proliferation detected with Ki67. Further studies are needed to understand the complex processes of angiogenesis, cell proliferation and apoptosis that interact in the placenta during porcine gestation.

show abstract