Porcine skin gelatin–silver nanocomposites: synthesis, characterisation, cell cytotoxicity, and antibacterial properties

Abstract

Silver nanoparticles (AgNPs) were synthesised with hydrothermal autoclaving technique by using AgNO salt (silver precursor) at different concentrations (0.01, 0.1, 0.55, 1.1, 5.5, and 11 mM) and porcine skin (1% (w/v) ) gelatin polymeric matrix (reducing and stabiliser agent). The reaction was performed in an autoclave at 103 kPa and 121°C and the hydrothermal autoclaving exposure time and AgNO molar concentration were varied at a constant porcine skin gelatin concentration. The as-prepared AgNPs were characterised by UV-visible spectroscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The antibacterial properties of AgNPs were tested against gram-positive and gram-negative bacteria. Furthermore, 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide and 2,2-diphenyl-1-picrylhydrazyl assays were used to test whether the synthesised AgNPs can be potentially applied in cancer therapy or used as an antioxidant. This approach is a promising simple route for synthesising AgNPs with a smaller average particle 10 nm diameter. Furthermore, AgNPs exhibited a good cytotoxicity activity, reducing the viability of the liver cancer cell line HepG2 with a moderate IC; they also showed a low-to-fair antioxidant activity. In addition, AgNPs had a remarkable preferential antibacterial activity against gram-positive bacteria than gram-negative bacteria. Therefore, these fabricated AgNPs can be used as an antibacterial agent in curative and preventive health care.

Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation

“…The balance between the biodegradation rate of PLGA (3-6 months) [6] and the period of neural regeneration (3 months) [7] has made it a suitable choice for fabrication of PNS Scaffolds. Besides, naturally derived polymers such as gelatine can compensate the hydrophobicity of polyesters by presenting amine and hydroxyl functional groups, so led to better cell-Scaffold interactions and rapid healing process [8][9][10][11]. Moreover, embedding the nanoparticles (NPs) in the Scaffolds can overcome the weak strength and induce new features to the constructs.…”

Stability of Biomimetically Functionalised Alginate Microspheres as 3D Support in Cell Cultures

“…Nevertheless, certain things can be stated about these spectra: first, the characteristic peaks of the moALG are also detected in all the coatings. The peak in the pure alginate and the moALG at 1620 cm −1 is related to the symmetrical ketone bonds present in the ALG [ 34 , 35 ]. The shift of this peak to 1632 cm −1 in the coated microspheres is significant.…”