Porcine Stentless Bioprostheses: Prevention of Aortic Wall Calcification by Dye‐Mediated Photo‐Oxidation

Meuris, Bart; Phillips, Richard E.; Moore, Mark A.; Flameng, Willem

doi:10.1046/j.1525-1594.2003.07108.x

Cited by 20 publications

(13 citation statements)

References 19 publications

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“…Dityrosine has been found to cross-link proteins and thus affects protein structure and function [21, 23, 24]. Photo-fixation for preparing clinical grade BHV has been attempted in the past by others; however, none of these BHV have proven to be suitable for clinical use [25, 26]. These prior efforts to induce photo-fixation crosslinks very likely resulted in the formation of dityrosine cross-links in BHV materials since photo-fixation as has been shown to form dityrosine [27].…”

Section: Discussionmentioning

confidence: 99%

The susceptibility of bioprosthetic heart valve leaflets to oxidation

et al. 2014

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The clinical use of bioprosthetic heart valves (BHV) is limited due to device failure caused by structural degeneration of BHV leaflets. In this study we investigated the hypothesis that oxidative stress contributes to this process. Fifteen clinical BHV that had been removed for device failure were analyzed for oxidized amino acids using mass spectrometry. Significantly increased levels of ortho-tyrosine, meta-tyrosine and dityrosine were present in clinical BHV explants as compared to the non-implanted BHV material glutaraldehyde treated bovine pericardium (BP). BP was exposed in vitro to oxidizing conditions (FeSO4/H2O2) to assess the effects of oxidation on structural degeneration. Exposure to oxidizing conditions resulted in significant collagen deterioration, loss of glutaraldehyde cross-links, and increased susceptibility to collagenase degradation. BP modified through covalent attachment of the oxidant scavenger 3-(4-hydroxy-3,5-di-tert-butylphenyl) propyl amine (DBP) was resistant to all of the monitored parameters of structural damage induced by oxidation. These results indicate that oxidative stress, particularly via hydroxyl radical and tyrosyl radical mediated pathways, may be involved in the structural degeneration of BHV, and that this mechanism may be attenuated through local delivery of antioxidants such as DBP.

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Section: Discussionmentioning

confidence: 99%

The susceptibility of bioprosthetic heart valve leaflets to oxidation

et al. 2014

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“…This phenomenon of tissue resistance to collagenase in the absence of high T d values was reported previously for the Photofix stabilization procedure. 33 Mechanical properties of PGG-treated scaffolds were similar to untreated and glutaraldehyde-treated samples except for a higher hysteresis. Despite effective collagen stabilization, PGG-treated collagen did not calcify in vivo, suggesting that the nature of the crosslinker may determine the outcome of collagenous implants.…”

mentioning

confidence: 82%

Stabilized Collagen Scaffolds for Heart Valve Tissue Engineering

Tedder

Liao

Weed

et al. 2009

Tissue Engineering Part A

124

111

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Scaffolds for heart valve tissue engineering must function immediately after implantation but also need to tolerate cell infiltration and gradual remodeling. We hypothesized that moderately cross-linked collagen scaffolds would fulfill these requirements. To test our hypothesis, scaffolds prepared from decellularized porcine pericardium were treated with penta-galloyl glucose (PGG), a collagen-binding polyphenol, and tested for biodegradation, biaxial mechanical properties, and in vivo biocompatibility. For controls, we used un-cross-linked scaffolds and glutaraldehyde-treated scaffolds. Results confirmed complete pericardium decellularization and the ability of scaffolds to encourage fibroblast chemotaxis and to aid in creation of anatomically correct valve-shaped constructs. Glutaraldehyde cross-linking fully stabilized collagen but did not allow for tissue remodeling and calcified when implanted subdermally in rats. PGG-treated collagen was initially resistant to collagenase and then degraded gradually, indicating partial stabilization. Moreover, PGG-treated pericardium exhibited excellent biaxial mechanical properties, did not calcify in vivo, and supported infiltration by host fibroblasts and subsequent matrix remodeling. In conclusion, PGG-treated acellular pericardium is a promising scaffold for heart valve tissue engineering.

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“…Of all factors currently considered as being responsible for prosthetic degeneration, by far, those which are the most important and are the most studied are calcification and the toxicity of glutaraldehyde [19][20][21][22][23]. Among the mechanisms involved in calcification, there is evidence that the presence of dead cells and cellular debris incompletely fixed by the treatment methods, work as calcification nuclei, permitting the deposition of calcium, sodium and magnesium phosphate and carbonate crystals [23].…”

Section: Discussionmentioning

confidence: 99%

Comparação entre o pericárdio bovino decelularizado e o pericárdio bovino convencional utilizado na confecção de biopróteses valvares cardíacas

Costa¹,

Pomerantzeff²,

Braile³

et al. 2005

Rev Bras Cir Cardiovasc

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Introduction: In this paper, our objective was to compare the decellularized and conventional pericardium mechanical resistance and also its capability of inducing inflammatory response in an animal experimental model.Methods: In order to study these properties, we divided the pericardia into two groups: Group I -pericardium conventionally treated with GTA and Group II -pericardium previously decellularized and then treated with GTA in the conventional way. After the chemical treatment, Group II samples were histologically evaluated to confirm the efficacy of the decellularization process. Then, only for the analysis of mechanical resistance, pericardia were divided in: Groups 1 (conventional pericardia with criteria of approval), 2 (conventional pericardial with criteria of rejection) and 3 (decellularized pericardia). The capacity of inducing inflammatory response was tested in a rat experimental model using 50 Wistar rats, in which rats of each group received patches of the pericardia in the abdomen. Our third step of analysis was to manufacture three decellularized pericardium bioprostheses which were submitted to hydrodynamic evaluation together with a conventional bioprosthesis test.Results: The histological analysis showed complete decellularization. Mechanical resistance gave statistical differences in the "tension of rupture" and "tenacity index" tests. We found no difference in the inflammatory activity in the animal model. Hydrodynamic performance was similar and all prostheses reached 150 million cycles. The final histological analysis assessed the standard microscopic pattern and no rupture or abnormal fragmentation was caused by mechanical stress. Conclusion:The decellularization technique maintains the physical resistance of the pericardium when compared with the conventionally prepared pericardium. And also, there was no difference in both groups regarding to inflammatory response studied in the animal model. Descriptors: Heart valve prosthesis. Bioprosthesis. Pericarduim. Comparative study. Comparação entre o pericárdio bovino decelularizado e o pericárdio bovino convencional utilizado na confecção de biopróteses valvares cardíacasComparison between the decellularized bovine pericardium and the conventional bovine pericardium used in the manufacture of cardiac bioprostheses 15 COSTA, JNL ET AL -Comparison between the decellularized bovine pericardium and the conventional bovine pericardium used in the manufacture of cardiac bioprostheses Braz J Cardiovasc Surg 2005; 20(1):14-22

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Porcine Stentless Bioprostheses: Prevention of Aortic Wall Calcification by Dye‐Mediated Photo‐Oxidation

Abstract: Photo-oxidation of a porcine stentless valve prevents calcification not only in the cusps but also in the aortic wall portion.

Cited by 20 publications

References 19 publications

The susceptibility of bioprosthetic heart valve leaflets to oxidation

The susceptibility of bioprosthetic heart valve leaflets to oxidation

Stabilized Collagen Scaffolds for Heart Valve Tissue Engineering

Comparação entre o pericárdio bovino decelularizado e o pericárdio bovino convencional utilizado na confecção de biopróteses valvares cardíacas

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