Pore Formation in Lipid Bilayer Membranes made of Phosphatidylcholine and Cholesterol Followed by Means of Constant Current

Naumowicz, Monika; Figaszewski, Zbigniew A.

doi:10.1007/s12013-012-9459-6

Cited by 23 publications

(25 citation statements)

References 53 publications

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“…The lowest value of R sp at -1.1 V of 10 4.8  cm 2 for the bilayer on Hg is somewhat lower than that recorded for lipid bilayers of > 10 5 [ 44] and 10 6 to 10 8 [45]  cm 2 and in this study this decreases exponentially by two orders of magnitude with increase in applied negative potential. The presence of electrolyte in the bilayer is confirmed by a finite increased permeability to Zn 2+ which increases with increase in applied negative potential.…”

Section: Discussioncontrasting

confidence: 68%

Phospholipid bilayers at the mercury (Hg)/water interface

Rashid

Vakurov

Nelson

2018

Electrochimica Acta

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This study reports on the electrochemical characterisation of dioleoyl phospatidylcholine (DOPC) bilayer structures on a negatively polarised mercury (Hg) electrode. The bilayers are stable on the Hg surface between-1.0 and-1.3 V applied potential. The experimental approaches were:-(i) Rapid cyclic voltammetry to "fingerprint" the bilayers, (ii) Potential step experiments to record Zn2+ reduction and, (ii) Electrochemical impedance. The results show the following. Both the specific capacitance (5 microF cm-2) and the specific resistance of the bilayer are higher and lower respectively than that of a defect-free free standing DOPC bilayer. This indicates the presence of water and ions in the bilayer within an applied negative field. The bilayer's resistance to electrolyte movement decreases with increase in negative potential to a minimum at-1.3 V. The DOPC bilayer is less permeable to Zn2+ ions compared to the DOPC monolayer coated electrode at applied negative potentials and its permeability to Zn2+ increases with an increase in negative applied potential. The specific capacitance of the bilayer increases to about 7.5 microF cm-2 with increase in applied negative potential showing the increasing significance of water in the bilayer commensurate with its increased permeability to ions. Adsorption of SiO2 nanoparticles on the bilayer surface causes a step negative potential shift in the anodic capacitance current bilayer reformation peak indicating an acceleration of the bilayer reformation process.

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Section: Discussioncontrasting

confidence: 68%

Phospholipid bilayers at the mercury (Hg)/water interface

Rashid

Vakurov

Nelson

2018

Electrochimica Acta

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“…Molecular dynamics simulations provide a view of one type of permeabilizing structure, the lipid electropore (Tieleman 2004; Gurtovenko and Vattulainen 2005; Tarek 2005; Böckmann et al 2008; Levine and Vernier 2010), consistent with experimental observations (Melikov et al 2001; Naumowicz and Figaszewski 2013; Koronkiwicz et al 2002) and continuum models (Zimmermann et al 1974; Abidor et al 1979; Chizmadzhev and Abidor 1980; Weaver and Chizmadzhev 1996). The formation of these nanometer-scale, conductive pores is initiated by the electric-field-stabilized intrusion of interfacial water into the membrane interior, which leads to the construction of energy-minimized water bridges across the lipid bilayer and the reorganization of the membrane phospholipids along the water columns to form the hydrophilic wall of the pore (Tokman et al 2013).…”

Section: Introductionsupporting

confidence: 75%

Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes

Vernier

Levine

et al. 2015

J Membrane Biol

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Nonthermal probing and stimulation with subnanosecond electric pulses and terahertz electromagnetic radiation may lead to new, minimally invasive diagnostic and therapeutic procedures and to methods for remote monitoring and analysis of biological systems, including plants, animals, and humans. To effectively engineer these still-emerging tools, we need an understanding of the biophysical mechanisms underlying the responses that have been reported to these novel stimuli. We show here that subnanosecond (≤ 500 ps) electric pulses induce action potentials in neurons and cause calcium transients in neuroblastoma-glioma hybrid cells, and we report complementary molecular dynamics simulations of phospholipid bilayers in electric fields in which membrane permeabilization occurs in less than 1 ns. Water dipoles in the interior of these model membranes respond in less than 1 ps to permeabilizing electric potentials by aligning in the direction of the field, and they re-orient at terahertz frequencies to field reversals. The mechanism for subnanosecond lipid electropore formation is similar to that observed on longer time scales — energy-minimizing intrusions of interfacial water into the membrane interior and subsequent reorganization of the bilayer into hydrophilic, conductive structures.

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“…the voltage required to breakdown a bilayer: several experimental studies characterized the impact of cholesterol contents for instance on the electroporation of simple lipid bilayers. [32][33][34][35] Most of the authors reported its stabilizing effect, as was later also found in MD simulation studies. 31,36 Yet, this seems not to be a universal behavior: in the case of diphytanoyl-glycerophosphocholine (DPhPC), lipid of which the saturated hydrocarbon chains are functionalized with methyl groups, cholesterol was found to slightly decrease the electroporation threshold.…”

Section: Introductionmentioning

confidence: 59%

Electroporation Threshold of POPC Lipid Bilayers with Incorporated Polyoxyethylene Glycol (C₁₂E₈)

et al. 2014

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Electroporation relates to a phenomenon in which cell membranes are permeabilized after being exposed to high electric fields. On the molecular level, the mechanism is not yet fully elucidated, although a considerable body of experiments and molecular dynamic (MD) simulations were performed on model membranes. Here we present the results of a combined theoretical and experimental investigation of electroporation of palmitoy-oleoyl-phosphatidylcholine (POPC) bilayers with incorporated polyoxyethylene glycol (C12E8) surfactants. The experimental results show a slight increase of the capacitance and a 22% decrease of the voltage breakdown upon addition of C12E8 to pure POPC bilayers. These results were qualitatively confirmed by the MD simulations. They later revealed that the polyoxyethylene glycol molecules play a major role in the formation of hydrophilic pores in the bilayers above the electroporation threshold. The headgroup moieties of the latter are indeed embedded in the interior of the bilayer, which favors formation of water wires that protrude into its hydrophobic core. When the water wires extend across the whole bilayer, they form channels stabilized by the C12E8 head groups. These hydrophilic channels can transport ions across the membrane without the need of major lipid head-group rearrangements.

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Pore Formation in Lipid Bilayer Membranes made of Phosphatidylcholine and Cholesterol Followed by Means of Constant Current

Cited by 23 publications

References 53 publications

Phospholipid bilayers at the mercury (Hg)/water interface

Phospholipid bilayers at the mercury (Hg)/water interface

Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes

Electroporation Threshold of POPC Lipid Bilayers with Incorporated Polyoxyethylene Glycol (C₁₂E₈)

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Pore Formation in Lipid Bilayer Membranes made of Phosphatidylcholine and Cholesterol Followed by Means of Constant Current

Cited by 23 publications

References 53 publications

Phospholipid bilayers at the mercury (Hg)/water interface

Phospholipid bilayers at the mercury (Hg)/water interface

Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes

Electroporation Threshold of POPC Lipid Bilayers with Incorporated Polyoxyethylene Glycol (C12E8)

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Product

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Electroporation Threshold of POPC Lipid Bilayers with Incorporated Polyoxyethylene Glycol (C₁₂E₈)