Pore-forming treatments induce aggregation of Salmonella Senftenberg through protein leakage

Hollander, Amit; Yaron, Sima

doi:10.1016/j.fm.2020.103721

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…Moreover, EOs are hydrophobic and can thus also increase cell hydrophobicity, favoring the adhesion of each cell with the neighbor. A further explanation was provided by Hollander and Yaron [ 35 ], who demonstrated how EOs and their hydrophobic constituents damage the cytoplasmic membrane, causing protein leakage that induces cell aggregation [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Antilisterial Effectiveness of Origanum vulgare var. hirtum and Coridothymus capitatus Essential Oils and Hydrolates Alone and in Combination

D’Amato,

Rossi,

Maggio

et al. 2024

Foods

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The antimicrobial activity of Origanum vulgare var. hirtum (O) and Coridothymus capitatus (C) essential oils (EOs) and hydrolates (HYs) of the same botanical species was evaluated on sixteen L. monocytogenes strains from food and clinical origins. The antimicrobial activity was assessed by Minimum Inhibitory Concentration (MIC) determination, viable cell enumeration over time up to 60 min, and evaluation of the cellular damage through Confocal Laser Scanning Microscope (CLSM) analysis. EOs exhibited antimicrobial activity with MIC values ranging from 0.3125 to 10 µL/mL. In contrast, HYs demonstrated antimicrobial effectiveness at higher concentrations (125–500 µL/mL). The effect of HYs was rapid after the contact with the cells, and the cell count reduction over 60 min of HY treatment was about 1.2–1.7 Log CFU/mL. L. monocytogenes cells were stressed by HY treatment, and red cell aggregates were revealed through CLSM observation. Moreover, the combinations of EOs and HYs had an additive antilisterial effect in most cases and allowed the concentration of use to be reduced, while maintaining or improving the antimicrobial effectiveness. The combined use of EOs and HYs can offer novel opportunities for applications, thereby enhancing the antimicrobial effectiveness and diminishing the concentration of use. This provides the added benefit of reducing toxicity and mitigating any undesirable sensory effects.

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Section: Resultsmentioning

confidence: 99%

Antilisterial Effectiveness of Origanum vulgare var. hirtum and Coridothymus capitatus Essential Oils and Hydrolates Alone and in Combination

D’Amato,

Rossi,

Maggio

et al. 2024

Foods

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“…A reason for this may be that lemon verbena essential oil is hydrophobic, which can increase the hydrophobicity of cells, thus making it conducive to the adhesion between cells. Yaron et al [ 38 ] found that the hydrophobic components of essential oil could destroy the cytoplasmic membrane, leading to protein leakage, thereby inducing cell aggregation, which may be due to the loss of transmembrane transporters such as SecA2 [ 39 ]. Lemon verbena essential oil can interact with the cytoplasmic membrane, resulting in damage to its structure and membrane proteins (such as SecA2).…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity and Antibacterial Mechanism of Lemon Verbena Essential Oil

Gao¹,

Liu²,

Li³

et al. 2023

Molecules

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The destructive effect and mode of action of lemon verbena essential oil on cells were investigated, taking the isolated Pseudosciaena D4 as the research object. The extracellular absorbance of the Pseudosciaena D4 increased at OD260 and OD280 after being treated with lemon verbena essential oil, which destroyed the integrity of Pseudosciaena D4 cells, showing a significant effect on preventing biomembrane formation and destroying the formed biomembrane. With an increased concentration of lemon verbena essential oil, extracellular polysaccharide showed a significant decrease in content and a significant increase in inhibition rate, indicating that the secretion of extracellular polysaccharide by Pseudosciaena D4 cells could be inhibited by lemon verbena essential oil during the process of biomembrane formation. Cell introcession and shrinkage appeared after the treatment with essential oil, and a transparent cavity was formed by the out-flowed cell content. Lemon verbena essential oil destroyed the cell wall, resulting in an enhanced permeability of the cell membrane and leakage of the contents, thereby causing cell death.

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“…Linalool acyclic monoterpene alcohol recognized as 3,7-dimethyl-1,6-octadien-3-ol, is a highly powerful compound identi ed in multiple crucial oils encompassing camphor oil (Cinnamomum camphora), basil oil (Ocimum basilicum), and lavender oil (Lavandula angustifolia) (Caputo et al, 2018;Hollander et al, 2021;Wang et al, 2019Wang et al, , 2020. Linalool possesses a range of pharmacologically bene cial properties, including anti-bacterial, in ammatory, analgesic, antioxidant, and anti-anxiety biological effects (Hsu et al, 2013;Mitic'-Cula c' et al, 2009;Li et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Linalool as a Potential Agent for Inhibiting Escherichia coli Biofilm Formation and Exopolysaccharide Production

Wang,

Zhang

et al. 2024

Preprint

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Linalool—a compound commonly found in various plant essential oils—exhibits multiple biological activities, encompassing anti-bacterial, inflammatory, analgesic, and antioxidant properties. However, the linalool implications on Escherichia coli (E. coli) biofilm (BF) remain underexplored. We proposed to ascertain the linalool implications on the development of E. coli BF and its extracellular polysaccharides, as well as to assess the implications of linalool on E. coli in both suspension and BF states. We discovered that the minimum biofilm inhibitory concentrations (MBICs) of linalool versus E. coli were twice as high as the lowest inhibitory concentrations. Linalool had a deadly impact on clinical E. coli strains obtained from cows with clinical endometritis, regardless of whether they were in a planktonic or BF condition. Linalool suppressed the BF development in a way that was dependent on the dosage, with an MBIC of 4 µL/mL. This was verified by the use of the crystal violet test and scanning electron microscopy. Moreover, the CCK-8 assay and confocal laser scanning microscopy (CLSM) manifested significant hindrances in live bacteria within the BF. The concentration of extracellular polymeric compounds in the E. coli BF was also mitigated. Furthermore, CLSM and RT-qPCR analysis confirmed that linalool (2 µL/mL) significantly suppressed exopolysaccharide (EPS) and the pgaABCD gene expression, regulating an essential exopolysaccharide expression in BF formation. These findings revealed that linalool effectively suppressed viable bacteria, EPS production, and E. coli BF formation, presenting a new method for preventing BF-related infections.

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Pore-forming treatments induce aggregation of Salmonella Senftenberg through protein leakage

Cited by 10 publications

References 42 publications

Antilisterial Effectiveness of Origanum vulgare var. hirtum and Coridothymus capitatus Essential Oils and Hydrolates Alone and in Combination

Antilisterial Effectiveness of Origanum vulgare var. hirtum and Coridothymus capitatus Essential Oils and Hydrolates Alone and in Combination

Antibacterial Activity and Antibacterial Mechanism of Lemon Verbena Essential Oil

Linalool as a Potential Agent for Inhibiting Escherichia coli Biofilm Formation and Exopolysaccharide Production

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