Pore network microarchitecture influences human cortical bone elasticity during growth and aging

Bala, Yohann; Lefevre, Emmanuelle; Roux, Jean-Paul; Baron, Cécile; Lasaygues, Philippe; Pithioux, Martine; Kaftandjian, Valérie; Follet, Hélène

doi:10.1016/j.jmbbm.2016.05.018

Cited by 31 publications

(29 citation statements)

References 69 publications

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“…On the other hand, a closed pore corresponds to the filling of bone tissue around a cortical pore. The open and the closed pores support the comprehension of total porosity (Bala et al 2016). In this study, groups G1 and G2, fed with a hyperphosphatemic diet, obtained average values on open and closed porosity that were superior to groups G3 and G4 with a balanced diet.…”

Section: Cortical Bone Analysissupporting

confidence: 61%

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Bone quality evaluation of experimental osteometabolic disease in Pantanal alligators (Caiman yacare) by High Resolution Computerized Microtomography (μCT)

et al. 2018

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Computerized microtomography is the gold standard examination for the evaluation of the three-dimensional bone structure. This experiment was developed to evaluate the structure and bone quality of Caiman yacare with metabolic bone disease using high resolution computerized microtomography (μCT). The animals were distributed into four groups: G1 -hyperphosphatemic diet with sun exposure deprivation (n=4), G2 -hyperphosphatemic diet with sun exposure (n=4), G3 -balanced diet with sun exposure deprivation (n=4), and G4 -balanced diet with exposure to sunlight (n=4). The parameters for the trabecular bone (Trabecular Number, Trabecular Thickness, Trabecular Separation, Bone Pattern Factor, Fractal Dimension, Euler Number, Structural Model Index, Degree of Anisotropy, Eigenvalues 1, 2 and 3, and Centroides X, Y and Z), and cortical bone (Number of Closed Pores, Volume of Closed Pores, Surface of Closed Pores, Closed Porosity, Volume of Open Pores, Open Porosity and Total Porosity). The overall results showed that the structure and bone quality of group G3 and G4 were better than those of groups G1 and G2, and that the diet factor influenced more than the sun exposure factor. The computerized microtomography allowed to evaluate the quality of the cortical and trabecular bones of the Pantanal alligator tibia with osteometabolic disease. The diet and sun exposure factors influenced individually the results of the μCT parameters between the groups, demonstrating the functional and structural complexity. Thus, these parameters can contribute to the interpretation of the mechanical behavior of bones and correlate them with the risk of lesions and fractures associated with osteometabolic diseases.

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Section: Cortical Bone Analysissupporting

confidence: 61%

“…On the other hand, increase in porosity is the main aspect of fracture risk (Bala et al 2016). For this experiment, the cortical bone analysis was performed within the assessment of bone porosity property.…”

Section: Cortical Bone Analysismentioning

confidence: 99%

Bone quality evaluation of experimental osteometabolic disease in Pantanal alligators (Caiman yacare) by High Resolution Computerized Microtomography (μCT)

et al. 2018

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“…Each VOI was chosen to be comparable to the specimen's volume in order to allow direct comparison between measured and calculated stiffness. Following Bala et al [12], the VOIs for the morphometric analysis were binarized treating the void volumes as the solid and the bone phase as a background ( figure 1d ). Then, the following microstrutural variables (table 1) were calculated using the software CTAnalyser (V 1.16.1, Skyscan NV, Kontich, Belgium): pore volume fraction (f ), pore surface to pore volume ratio (PoS/PoV), the average diameter of the pores (PoDm), the average separation between pores (PoSp), pore number (PoN), connectivity density (ConnD), pore pattern factor (PoPf ), structure model index (SMI) and degree of anisotropy (DA).…”

Section: Bone Microstructurementioning

confidence: 99%

Homogenization of cortical bone reveals that the organization and shape of pores marginally affect elasticity

Cai

Brenner

Peralta

et al. 2019

J. R. Soc. Interface.

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With ageing and various diseases, the vascular pore volume fraction (porosity) in cortical bone increases, and the morphology of the pore network is altered. Cortical bone elasticity is known to decrease with increasing porosity, but the effect of the microstructure is largely unknown, while it has been thoroughly studied for trabecular bone. Also, popular micromechanical models have disregarded several micro-architectural features, idealizing pores as cylinders aligned with the axis of the diaphysis. The aim of this paper is to quantify the relative effects on cortical bone anisotropic elasticity of porosity and other descriptors of the pore network micro-architecture associated with pore number, size and shape. The five stiffness constants of bone assumed to be a transversely isotropic material were measured with resonant ultrasound spectroscopy in 55 specimens from the femoral diaphysis of 29 donors. The pore network, imaged with synchrotron radiation X-ray micro-computed tomography, was used to derive the pore descriptors and to build a homogenization model using the fast Fourier transform (FFT) method. The model was calibrated using experimental elasticity. A detailed analysis of the computed effective elasticity revealed in particular that porosity explains most of the variations of the five stiffness constants and that the effects of other micro-architectural features are small compared to usual experimental errors. We also have evidence that modelling the pore network as an ensemble of cylinders yields biased elasticity values compared to predictions based on the real micro-architecture. The FFT homogenization method is shown to be particularly efficient to model cortical bone.

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“…Both of these techniques facilitate the observation of canals but only on sections of bone. More recently, the three-dimensional vascular network has been largely studied in cortical bone mostly using micro-computed tomography as it allows the investigation on large volumes (Bala et al, 2016;Bousson et al, 2004;Chappard et al, 2006;Cooper et al, 2007). It seems important to investigate such a network in 3D as it has been shown that the orientation of these canals may be associated to their mechanical environment (Hennig et al, 2015;Heřt et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

3D micro structural analysis of human cortical bone in paired femoral diaphysis, femoral neck and radial diaphysis

Gauthier

Langer

Follet

et al. 2018

Journal of Structural Biology

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Human bone is known to adapt to its mechanical environment in a living body. Both its architecture and microstructure may differ between weight-bearing and non-weight-bearing bones. The aim of the current study was to analyze in three dimensions, the morphology of the multi-scale porosities on human cortical bone at different locations. Eight paired femoral diaphyses, femoral necks, and radial diaphyses were imaged using Synchrotron Radiation µCT with a 0.7 µm isotropic voxel size. The spatial resolution facilitates the investigation of the multiscale porosities of cortical bone, from the osteonal canals system down to the osteocyte lacunar system. Our results showed significant differences in the microstructural properties, regarding both osteonal canals and osteocytes lacunae, between the different anatomical locations. The radius presents significantly lower osteonal canal volume fraction and smaller osteonal canals than the femoral diaphysis or neck. Osteocytes lacunae observed in the radius are significantly different in shape than in the femur, and lacunar density is higher in the femoral neck. These results show that the radius, a non-weight-bearing bone, is significantly different in terms of its microstructure from a weight-bearing bone such as the femur. This implies that the cortical bone properties evaluated on the femoral diaphysis, the main location studied within the literature, cannot be generalized to other anatomical locations.

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Pore network microarchitecture influences human cortical bone elasticity during growth and aging

Cited by 31 publications

References 69 publications

Bone quality evaluation of experimental osteometabolic disease in Pantanal alligators (Caiman yacare) by High Resolution Computerized Microtomography (μCT)

Bone quality evaluation of experimental osteometabolic disease in Pantanal alligators (Caiman yacare) by High Resolution Computerized Microtomography (μCT)

Homogenization of cortical bone reveals that the organization and shape of pores marginally affect elasticity

3D micro structural analysis of human cortical bone in paired femoral diaphysis, femoral neck and radial diaphysis

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