Pore-Size Controlled and Poly(?-Lysine)-Immobilized Cellulose Spherical Particles for Removal of Lipopolysaccharides

Todokoro, Masami; Sakata, Masayo; Matama, Satoshi; Kunitake, Masashi; Ohkuma, Kunio; Hirayama, Chuichi

doi:10.1081/jlc-120003349

Cited by 12 publications

(25 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously reported 8,9 that the adsorption activity of PL-Cellufine for bio-products is due to the simultaneous effects of their cationic and hydrophobic or other properties. PL-Cellufine and DEAE-Sepharose, being cationic in nature, can adsorb LPS and acidic proteins mainly by ionic interaction, because the charge of LPS (pK1 = 1.3) 13 and acidic proteins (ovalbumin (pI = 4.6), BSA (pI = 4.9)) are anionic at neutral pH.…”

Section: Selective Adsorption Of Lps Onto Pl-cellufinementioning

confidence: 99%

“…The samples were dissolved in one of the following buffers: 0.02 M sodium acetate (pH 4, 5), 0.02 M phosphate (pH 6,7,8), and 0.02 M Tris (pH 9), and the pH and ionic strength were adjusted. The ionic strength of the buffer was adjusted by changing the sodium chloride concentration.…”

Section: Selective Adsorption Of Lps On Pl-cellufinementioning

confidence: 99%

“…Poly(ε-lysine)-immobilized cellulose beads (PL-Cellufine) [8][9][10] was obtained from Chisso Co. Ltd. (Tokyo, Japan). PL (Mw, 4500; pKa, 7.6; Chisso) was produced by Streptomyces albulus.…”

Section: Reagents and Materialsmentioning

confidence: 99%

See 2 more Smart Citations

Limulus Amebocyte Lysate Assay for Endotoxins by an Adsorption Method with Polycation-immobilized Cellulose Beads

et al. 2010

Self Cite

View full text Add to dashboard Cite

introductionThe pharmaceutical products for parenteral administration must be tested for contamination of bacterial endotoxins (LPSs 1 ) because of their potent biologic activities, which can cause pyrogenic and shock reactions. LPS, a constituent of the cell walls of Gram-negative bacteria, is a potential contaminant of protein solutions originating from biologic products. There are two methods for determining LPS. One is the rabbit pyrogen test; the other is the limulus test using Limulus amebocyte lysate (LAL).The gel-clot, 2 turbidimetric, 3 and chromogenic 4 testing methods, methods previously approved by the USA Pharmacopoeia in 1981, 1985, and 1985, respectively, are typically employed in the LAL test. The advantages of the LAL test over the rabbit pyrogen test are higher sensitivity, lower cost, and higher reproducibility. The LAL test is inhibited or enhanced by antibiotics, hormones, heavy metals, amino acids, alkaloids, plasma proteins, enzymes, and electrolytes in the sample solution.5 Dilution, heating and dialysis have been used to overcome these problems, but the results have been unsatisfactory. Minobe et al. attempted to develop a new specific assay method for LPSs using histidine-immobilized Sepharose as an adsorbent.6,7 LPSs adsorbed by the adsorbent were separated from the compounds (e.g., amino acids, enzymes) in solution by centrifugation, and the adsorbed LPSs can react directly with the LAL. The great disadvantage of this type of adsorbent is the low adsorption activity of the LPSs in physiologic solution (neutral pH and ionic strength (μ) = 0.1 -0.2).In this study, we developed a novel assay of LPSs with a new adsorption method using a turbidimetric time assay system. We previously found that poly(ε-lysine)-immobilized cellulose beads (PL-Cellufine) can selectively adsorb LPSs in bioproducts over a wide range of ionic strengths (μ) from 0.05 -0.8 8-10 at neutral pH. On the other hand, a simple turbidimetric time assay system has been developed for the quantitative assaying of LPS; an automated apparatus is available commercially. 11In this paper, we describe the LPS-adsorbing activities of PL-Cellufine. Then, using the turbidimetric time assay, we describe the optimum conditions for the LPS assay using the PL-Cellufine as adsorbent. We also compare the apparent quantity of LPS provided by the adsorption method with the apparent quantity of LPS provided by a normal method. experimental Reagents and materialsPurified LPS (Escherichia coli UKT-B and O111:B4) and Limulus ES-II test Wako (Limulus amebocyte lysate) were purchased from Wako Pure Chemicals (Osaka, Japan). Poly(ε-lysine)-immobilized cellulose beads (PL-Cellufine) [8][9][10] was obtained from Chisso Co. Ltd. (Tokyo, Japan). PL (Mw, 4500; pKa, 7.6; Chisso) was produced by Streptomyces albulus. 12 DEAE-Sepharose CL-6B was purchased from GE Healthcare Bio-Sciences Corp. (USA). Ovalbumin (from egg), bovine serum albumin (BSA), γ-globulin (from human serum), lysozyme (from horse heart), and vitamin K3 were purchased from Wako. Myoglobi...

show abstract

Section: Selective Adsorption Of Lps Onto Pl-cellufinementioning

confidence: 99%

Section: Selective Adsorption Of Lps On Pl-cellufinementioning

confidence: 99%

See 1 more Smart Citation

Limulus Amebocyte Lysate Assay for Endotoxins by an Adsorption Method with Polycation-immobilized Cellulose Beads

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Notes chloromethyloxirane (CMO) were purchased from Wako. Poly(ε-lysine)-immobilized cellulose beads 4 (PL-Cellufine, sold as ETclean-L from Chisso Co. Ltd., Tokyo, Japan) and DEAE-Sepharose CL-6B (GE Healthcare Bio-Sciences Corp., USA) were used as LPS adsorbents. The distilled water (Otsuka Pharmaceiticals Co., Tokyo, Japan) was used as LPS-free water.…”

Section: Reagents and Materialsmentioning

confidence: 99%

“…Removal of the LPS from substances used as drugs is very important, since it causes pyrogenic and shock reactions in mammals upon intravenous injection, even in nanogram quantities. 2 Already, various cationic polymer adsorbents, such as polymyxin-immobilized Sepharose 3 (Detoxi-Gel ® , Pierce Chemical) and poly(ε-lysine)-immobilized Cellufine 4 (ETclean ® , Chisso Co. Ltd.), have been used for removing LPS from bio-product solutions. However, it has been reported 5 that these cationic adsorbents cannot selectively remove LPS from acidic substances, such as DNA, because of the high adsorption for both LPS and DNA.…”

Section: Introductionmentioning

confidence: 99%

Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads

et al. 2011

Self Cite

View full text Add to dashboard Cite

Optimization of the Endotoxin Removal Performance of Solid-Phase Conjugated S3E3 Antimicrobial Peptide Using Response Surface Methodology

Sepahi

Norouzian

Cohan

et al. 2021

Int J Pept Res Ther

View full text Add to dashboard Cite

Pore-Size Controlled and Poly(?-Lysine)-Immobilized Cellulose Spherical Particles for Removal of Lipopolysaccharides

Cited by 12 publications

References 15 publications

Limulus Amebocyte Lysate Assay for Endotoxins by an Adsorption Method with Polycation-immobilized Cellulose Beads

Limulus Amebocyte Lysate Assay for Endotoxins by an Adsorption Method with Polycation-immobilized Cellulose Beads

Selective Removal of Endotoxin from a DNA Solution by Cross-linked Cyclodextrin Beads

Optimization of the Endotoxin Removal Performance of Solid-Phase Conjugated S3E3 Antimicrobial Peptide Using Response Surface Methodology

Contact Info

Product

Resources

About