2000
DOI: 10.1016/s0021-9673(00)00420-9
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Pore size distributions of cation-exchange adsorbents determined by inverse size-exclusion chromatography

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Cited by 266 publications
(199 citation statements)
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“…The accessible particle porosity ε p could not be determined directly because the quantities of adsorbent were insufficient, but it can be estimated from the maximum static capacities in the adsorption isotherms on the charge variants, SP-550C and SP-650C by assuming that these capacities scale linearly with the accessible surface area per particle volume, which depends on the pore size distribution [16,18,33]; this in turn provides a relation to the accessible porosity. The accessible porosities of SP-550 C and SP-650 M are 0.29 and 0.49 respectively for a molecule of 1.6 nm in radius [34], which provides an estimate approximately in the range 0.35-0.4 for the accessible porosity of the adsorbents studied here.…”
Section: Protein Uptake By Confocal Microscopymentioning
confidence: 59%
See 1 more Smart Citation
“…The accessible particle porosity ε p could not be determined directly because the quantities of adsorbent were insufficient, but it can be estimated from the maximum static capacities in the adsorption isotherms on the charge variants, SP-550C and SP-650C by assuming that these capacities scale linearly with the accessible surface area per particle volume, which depends on the pore size distribution [16,18,33]; this in turn provides a relation to the accessible porosity. The accessible porosities of SP-550 C and SP-650 M are 0.29 and 0.49 respectively for a molecule of 1.6 nm in radius [34], which provides an estimate approximately in the range 0.35-0.4 for the accessible porosity of the adsorbents studied here.…”
Section: Protein Uptake By Confocal Microscopymentioning
confidence: 59%
“…Intraparticle structural information can be obtained under realistic chromatographic conditions using inverse size exclusion chromatography [34,39,40], but insufficient quantities of the charge variant materials were available for reliable measurements. Instead, local rather than averaged information was obtained by TEM.…”
Section: Structural Characterizationmentioning
confidence: 99%
“…The properties of the tested chromatography media are summarized in Table 1. Pore diameter, pore volumes and ligand densities were either from previous measurements [26,27], or specifications of manufacturers, or own measurements, respectively. The external and internal surface areas were roughly estimated by simplified equations, implying the particle diameter and the extraparticle porosity (i. e. void fraction) for A ext , and additionally the pore volume (i. e. intraparticle porosity) for A int .…”
Section: Resultsmentioning
confidence: 99%
“…B HS is the excluded volume contribution by the protein as a hard sphere (calculated based on the molecular weight of the protein). Finally, the phase ratio, ', is the ratio of available surface area to available volume and is an established value dependent on the medium (DePhillips & Lenhoff, 2000). A Pierce BCA (protein concentration) assay is used to determine , the number of covalently bound protein molecules per unit area of column medium.…”
Section: Reviewmentioning
confidence: 99%