Poria cocos Modulates Th1/Th2 Response and Attenuates Airway Inflammation in an Ovalbumin-Sensitized Mouse Allergic Asthma Model

Chao, Chia-Chen; Wang, Chao-Jih; Huang, Hsin-Wen; Kuo, Han-Peng; Su, Muh-Hwan; Lin, Hang-Ching; Teng, Chia-Wen; Sy, Leticia B.; Wu, Wen-Mein

doi:10.3390/life11050372

“…For example, Zhang et al 20 found that Cortex Moutan ameliorated the inflammation in advanced glycation end products-induced mesangial cell dysfunction and high-glucose-fat diet and streptozotocin-induced diabetic nephropathy rats. Chao et al 21 reported that Poria cocos extract attenuated airway inflammation and prevented allergy-induced asthma or symptoms. Kim et al 22 described that ethanol extract of Alisma orientale significantly improved lung inflammation and enhanced the survival of mice that received a lethal dose of lipopolysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Study of Herbs Cortex Moutan, Poria cocos, and Alisma orientale and Periodontitis

Hu,

Su

2024

International Dental Journal

1

0

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“…Under the impact of IFN- γ , NKs can activate T cells making a further increase of IFN- γ instead of IL-4 [ 38 ]. This means that the function of NKs for asthma is in reference to the cytokine microenvironment [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Majie Cataplasm Promotes Th1 Response to Fight against Asthmatic Th2 Inflammation through NKs

Ji

¹

,

Shi

²

,

Feng

³

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Immune cells are tightly bound up with the pathogenesis of asthma. Besides T cells, B cells, macrophages, and mast cells, the mechanism of innate lymphoid cells (ILCs) in asthma is gradually explicit. As a kind of traditional Chinese medicine, Majie cataplasm realizes its potential in the clinical setting as an adjuvant for asthma. In our previous experiments, Majie cataplasm inhibits the increasing Th1 and Th2 in allergic asthma inflammation and reshapes a balance between Th1 and Th2. As ILCs are the reflection of Th cells in lung tissues, we will figure out whether Majie cataplasm could have similar effects on ILCs or not. Methods. A total of 40 female C57/BL6 mice were randomly divided into the control group (n = 10), the asthma model group (n = 10), the dexamethasone group (n = 10), and the Majie cataplasm group (n = 10). Except for the control group, mice were sensitized with ovalbumin (OVA) and excited to establish mice models of asthma. Lung tissue and splenic tissue were collected at 24 h after the last challenge with OVA, and the cell suspension of the lungs and spleen was prepared. The number of ILC1s, ILC2s, ILC3s, and NKs cells in the lungs and Tregs and B10s in the spleen were detected by flow cytometry (FCM). This was followed by simultaneous quantitative detection of 40 inflammatory cytokines and chemokines in the lung by a protein microarray. Results. The dexamethasone and Majie cataplasm could restore the number of ILC1s, ILC2s, and ILC3s in lung tissue. Compared with the control group, these cells remained unchanged in the asthma model group, while ILC1s ( P < 0.001 , P < 0.01 ), ILC2s ( P < 0.001 , P < 0.01 ), and ILC3s ( P < 0.01 , P < 0.05 ) were restored after the intervention of dexamethasone and Majie cataplasm. The number of NKs was low among the control group, the asthma model group, and the dexamethasone group, while the number of NKs rocketed in the Majie cataplasm group ( P < 0.0001 ). For splenic Tregs and B10s, Majie cataplasm could curb the increasing numbers of them in the asthma model group ( P < 0.0001 , P < 0.01 ), while only Tregs were suppressed by the dexamethasone ( P < 0.0001 ). For the inflammatory cytokines in the lung, the contents of TNF-α, TNFR2, CXCL-9, CCL-12, CCL-9, CCL-2, and CCL-5 in the asthma model group were higher than those in the control group, while the contents of GM-CSF and IL-1α were decreased. Comparing the asthma model group to the dexamethasone group, the levels of G-CSF, CCL-9, CCL-5, and TNFR2 in the former group were higher. The levels of TNF-α, TNFR2, and CCL-9 in the asthma model group increase, while the levels of IFN-γ, IL-1α, ICAM-1, and IL-4 increased in the Majie cataplasm group, especially IFN-γ and IL-1α. Conclusion. Both the dexamethasone and Majie cataplasm could control the asthmatic inflammation by reducing the inflammatory factors, inhibiting the adaptive inflammation reaction in the latter stage of inflammation and furtherly reversing the inhibition of ILC2s, ILC2s, and ILC3s. In addition, Majie cataplasm can promote the quantity of NKs and the content of IL-1α and IFN-γ, induce IFN-γ+NKs to shut down the Th2 response, and tend to elicit the Th1 response.

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“…In this study, a special self-prescription LHT of Menghe for treating "Bi syndrome" (RA), was selected from the book "Yi Chun Sheng Yi (1863)". According to the literature, Cistanche deserticola [10], Codonopsis pilosula [11], Poria cocos [12], Atractylodes macrocephala Koidz [13], Angelica sinensis [14] and other drugs in LHT have the effects of regulating immunity and anti-inflammation, Radix Paeoniae Alba has immunosuppressive function and been widely used in the treatment of RA and related research [15,16]. The results of this study show that LHT can significantly delay the occurrence of joint inflammation, reduce the inflammation score and joint swelling, and prevent joint damage and deformation in rats.…”

Section: Discussionmentioning

confidence: 99%

The pharmacological mechanism study of traditional prescription Meng-He Long-Huo-Tang on the treatment of rheumatoid arthritis

2022

FMSR

0

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A Chinese traditional prescription Meng-He Long-Huo-Tang (LHT) has been used for the treatment of rheumatoid arthritis (RA) for long time, but the pharmacological mechanism is unknown. A collagen-induced arthritis (CIA) rat model was established to study the effects of LHT on CIA rats by recording body weight, paw thickness, arthritis score and peripheral blood analysis. In vitro, rat fresh synovial fibroblasts (SFbs) were extracted, and the effects of LHT on SFbs proliferation, migration and apoptosis were investigated using cell viability and migration assays, gelatin zymography, western blot and immunocytochemistry. In vivo experiments show that LHT can reduce the joint swelling, decrease the levels of MMP-2/9 and inflammatory factors in the blood of CIA rats. In vitro experiments showed that LHT inhibited cell proliferation, migration and promoted apoptosis of activated SFbs. To sum up, this study revealed the underlying mechanism that LHT can effectively alleviate rheumatoid arthritis symptoms by reducing inflammatory factors and inhibiting SFbs excessive proliferation.

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Poria cocos Modulates Th1/Th2 Response and Attenuates Airway Inflammation in an Ovalbumin-Sensitized Mouse Allergic Asthma Model

Cited by 17 publications

References 37 publications

Study of Herbs Cortex Moutan, Poria cocos, and Alisma orientale and Periodontitis

Study of Herbs Cortex Moutan, Poria cocos, and Alisma orientale and Periodontitis

Majie Cataplasm Promotes Th1 Response to Fight against Asthmatic Th2 Inflammation through NKs

The pharmacological mechanism study of traditional prescription Meng-He Long-Huo-Tang on the treatment of rheumatoid arthritis

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