Poria cocos water extract ameliorates the behavioral deficits induced by unpredictable chronic mild stress in rats by down-regulating inflammation

Huang, Yun-Ju; Hsu, Nai-Yuan; Lu, Kuan-Hung; Lin, Yan; Lin, Shih-Hang; Lu, Yun-Sheng; Liu, Wei-Ting; Chen, Mei-Hsing; Sheen, Lee‐Yan

doi:10.1016/j.jep.2020.112566

Cited by 34 publications

(26 citation statements)

References 30 publications

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“…P. cocos has been proposed to treat insomnia through the neurotransmitter γ -aminobutyric acid or by stimulating the γ -aminobutyric acid AA receptor [ 32 ]. P. cocos water extracts have also been suggested as herbal medicines for treating depression [ 33 ]. A recent study reported that P. cocos polysaccharides exhibited neuroprotective effects by regulating various cell functions in an Alzheimer's disease rat model [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Poria cocos Regulates Cell Migration and Actin Filament Aggregation in B35 and C6 Cells by Modulating the RhoA, CDC42, and Rho Signaling Pathways

Lee

Tzeng

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Poria is used as a traditional Chinese herbal medicine with anti-inflammatory, anticancer, and mood-stabilizing properties. Poria contains triterpenoids and polysaccharides, which are reported to regulate the cytoplasmic free calcium associated with the N-methyl-D-aspartate receptor and affect the cell function of neonatal rat nerve cells and hippocampal neurons. Although the modulatory effects of Poria on neuronal function have been widely reported, the molecular mechanism of these effects is unclear. Cell migration ability and the reorganization of actin filaments are important biological functions during neuronal development, and they can be regulated mainly by the Rho signaling pathway. We found that the cell migration ability and actin condensation in B35 cells enhanced by P. cocos (a water solution of P. cocos cum Radix Pini (PRP) or White Poria (WP)) might be caused by increased RhoA and CDC42 activity and increased expression of downstream ROCK1, p-MLC2, N-WASP, and ARP2/3 in B35 cells. Similar modulations of cell migration ability, actin condensation, and Rho signaling pathway were also observed in the C6 glial cell line, except for the PRP-induced regulation of RhoA and CDC42 activities. Ketamine-induced inhibition of cell migration and actin condensation can be restored by P. cocos. In addition, we observed that the increased expression of RhoA and ROCK1 or the decreased expression of CDC42 and N-WASP caused by ketamine in B35 cells could also be restored by P. cocos. The results of this study suggest that the regulatory effects of P. cocos on cell migration and actin filament aggregation are closely related to the regulation of RhoA, CDC42, and Rho signaling pathways in both B35 and C6 cells. PRP and WP have the potential to restore neuronal cell Rho signaling abnormalities involved in some mental diseases.

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Section: Discussionmentioning

confidence: 99%

Poria cocos Regulates Cell Migration and Actin Filament Aggregation in B35 and C6 Cells by Modulating the RhoA, CDC42, and Rho Signaling Pathways

Lee

Tzeng

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…[101][102][103] These include various natural herbs and/or their ingredients, such as Centella asiatica, Poria cocos, Magnoliae cortex, and Anemarrhenae rhizome that showed anti-inflammatory effects and alleviated stress-induced behaviors. [104][105][106][107][108][109] Centella asiatica revealed anxiolytic, antidepressant, and cognitive improvement effects in mice subjected to chronic stress, potentially via down-regulation of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α in the hippocampus, and inhibition of NF-κBand NLRP3-related inflammatory pathways. [104][105][106] Poria cocos, a medicinal mushroom that has been used in traditional medicine prescriptions and proven to have anti-inflammatory properties, alleviated behavioral abnormalities induced by unpredictable chronic mild stress.…”

Section: Clinical Neurosciencesmentioning

confidence: 99%

“…[104][105][106] Poria cocos, a medicinal mushroom that has been used in traditional medicine prescriptions and proven to have anti-inflammatory properties, alleviated behavioral abnormalities induced by unpredictable chronic mild stress. 107 Although their mechanisms of action are still largely unknown and further randomized and placebo-controlled studies are needed to confirm their potentials as remedies for depression, findings above suggest that natural products may be useful tools to uncover novel therapeutic targets for depression by deciphering their mechanisms utilizing modern neuroscience approaches.…”

Section: Clinical Neurosciencesmentioning

confidence: 99%

Inflamed brain: Targeting immune changes and inflammation for treatment of depression

Sakamoto

Zhu

Hasegawa

et al. 2021

Psychiatry Clin Neurosci

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Although there are a number of clinically effective treatments for depression, many patients exhibit treatment resistance. Recent clinical and preclinical studies reveal that peripheral and brain immune changes and inflammation are involved in the pathophysiology of depression. This 'Inflamed Brain' research provides critical clues for understanding of disease pathophysiology and many candidate molecules that are potentially useful for identifying novel drug targets for the treatment of depression. In this review, we will present clinical evidence on the role of inflammation in the pathophysiology of depression. We will also summarize current clinical trials which test drugs targeting inflammation for the treatment of patients with depression. Furthermore, we will briefly provide preclinical evidence demonstrating altered immune system function and inflammation in stress-induced animal models and will discuss the future potential of inflammationrelated drug targets. Collectively, inflammatory signatures identified in clinical and preclinical studies may allow us to stratify depressive patients based on biotypes, contributing to the development of novel mechanism-based interventions that target specific patient populations.

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“…(English: Atractylodis macrocephalae Rhizoma, Chinese: Bai Zhu) Immune and anti-inflammatory activity ( Ruqiao et al, 2020 ) 9 Poria cocos (Schw.) Wolf (English: Poria, Chinese: Fu Ling) Anti-inflammatory, antioxidant, and antiviral activities ( Huang et al, 2020a , Huang et al, 2020b ) 10 Bupleurum chinense DC. (English: Bupleuri Radix, Chinese: Chai Hu) Evacuating fever and pyretolysis, soothing liver and relieving depression and lifting the spirit ( Yang et al, 2017 ) 11 Scutellaria baicalensis Georgi (English: Scutellariae Radix, Chinese: Huang Qin) Antipyretic, hemostatic, virus-related diseases ( Ming et al, 2007 ) 12 Pinellia ternata (Thunb.)…”

Section: Introductionmentioning

confidence: 99%

“…The COVID-19 patients showed mild or acute respiratory syndrome accompanied by release of pro-inflammatory cytokines ( Conti et al, 2020 ). Besides, cytokine storm is associated with clinical deterioration of COVID-19 ( Huang et al, 2020a , Huang et al, 2020b ). Therefore, suppression of inflammatory, immunity regulation and relaxation of airway smooth muscle may have therapeutic effect on COVID-19.…”

Section: Introductionmentioning

confidence: 99%

Mechanism deconvolution of Qing Fei Pai Du decoction for treatment of Coronavirus Disease 2019 (COVID-19) by label-free integrative pharmacology assays

Hou

Shen

et al. 2021

Journal of Ethnopharmacology

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Ethnopharmacological relevance Traditional Chinese medicine (TCM) has a long history in the prevention and treatment of pandemics. The TCM formula Lung Cleansing and Detoxifying Decoction (LCDD), also known as Qing Fei Pai Du Decoction, has been demonstrated effective against Coronavirus Disease 2019 (COVID-19). Aim of the study This work aimed to elucidate the active ingredients, targets and pathway mechanism of LCDD related to suppression of inflammatory, immunity regulation and relaxation of airway smooth muscle for the treatment of COVID-19. Materials and methods Mining chemical ingredients reported in LCDD, 144 compounds covering all herbs were selected and screened against inflammatory-, immunity- and respiratory-related GPCRs including GPR35, H1, CB2, B2, M3 and β2-adrenoceptor receptor using a label-free integrative pharmacology method. Further, all active compounds were detected using liquid chromatography-tandem mass spectrometry, and an herb-compound-target network based on potency and content of compounds was constructed to elucidate the multi-target and synergistic effect. Results Thirteen compounds were identified as GPR35 agonists, including licochalcone B, isoliquiritigenin, etc. Licochalcone B, isoliquiritigenin and alisol A exhibited bradykinin receptor B2 antagonism activities. Atractyline and shogaol showed as a cannabinoid receptor CB2 agonist and a histamine receptor H1 antagonist, respectively. Tectorigenin and aristofone acted as muscarinic receptor M3 antagonists, while synephrine, ephedrine and pseudoephedrine were β2-adrenoceptor agonists. Pathway deconvolution assays suggested activation of GPR35 triggered PI3K, MEK, JNK pathways and EGFR transactivation, and the activation of β2-adrenoceptor mediated MEK and Ca 2+ . The herb-compound-target network analysis found that some compounds such as licochalcone B acted on multiple targets, and multiple components interacted with the same target such as GPR35, reflecting the synergistic mechanism of Chinese medicine. At the same time, some low-abundance compounds displayed high target activity, meaning its important role in LCDD for anti-COVID-19. Conclusions This study elucidates the active ingredients, targets and pathways of LCDD. This is useful for elucidating multitarget synergistic action for its clinical therapeutic efficacy.

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Poria cocos water extract ameliorates the behavioral deficits induced by unpredictable chronic mild stress in rats by down-regulating inflammation

Cited by 34 publications

References 30 publications

Poria cocos Regulates Cell Migration and Actin Filament Aggregation in B35 and C6 Cells by Modulating the RhoA, CDC42, and Rho Signaling Pathways

Poria cocos Regulates Cell Migration and Actin Filament Aggregation in B35 and C6 Cells by Modulating the RhoA, CDC42, and Rho Signaling Pathways

Inflamed brain: Targeting immune changes and inflammation for treatment of depression

Mechanism deconvolution of Qing Fei Pai Du decoction for treatment of Coronavirus Disease 2019 (COVID-19) by label-free integrative pharmacology assays

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