Porifera (Sponges)–5

Kornprobst, Jean‐Michel

doi:10.1002/9783527335855.marprod195

Cited by 2 publications

(1 citation statement)

References 4,431 publications

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“…The high diversity of sponge-associated prokaryotes, each producing its own specific set of compounds, has been used to partially explain the exceptional diversity of chemical compounds in sponges 14 , 15 and positions them as leading sources for novel marine bioactive compounds 16 , 17 . However, the extent of the contribution of microbes to the holobiont chemodiversity is still poorly understood and a topic of high interest.…”

Section: Introductionmentioning

confidence: 99%

Oceanographic setting influences the prokaryotic community and metabolome in deep-sea sponges

Steffen

Indraningrat

Erngren

et al. 2022

Sci Rep

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Marine sponges (phylum Porifera) are leading organisms for the discovery of bioactive compounds from nature. Their often rich and species-specific microbiota is hypothesised to be producing many of these compounds. Yet, environmental influences on the sponge-associated microbiota and bioactive compound production remain elusive. Here, we investigated the changes of microbiota and metabolomes in sponges along a depth range of 1232 m. Using 16S rRNA gene amplicon sequencing and untargeted metabolomics, we assessed prokaryotic and chemical diversities in three deep-sea sponge species: Geodia barretti, Stryphnus fortis, and Weberella bursa. Both prokaryotic communities and metabolome varied significantly with depth, which we hypothesized to be the effect of different water masses. Up to 35.5% of microbial ASVs (amplicon sequence variants) showed significant changes with depth while phylum-level composition of host microbiome remained unchanged. The metabolome varied with depth, with relative quantities of known bioactive compounds increasing or decreasing strongly. Other metabolites varying with depth were compatible solutes regulating osmolarity of the cells. Correlations between prokaryotic community and the bioactive compounds in G. barretti suggested members of Acidobacteria, Proteobacteria, Chloroflexi, or an unclassified prokaryote as potential producers.

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Section: Introductionmentioning

confidence: 99%

Oceanographic setting influences the prokaryotic community and metabolome in deep-sea sponges

Steffen

Indraningrat

Erngren

et al. 2022

Sci Rep

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Barrettides: A Peptide Family Specifically Produced by the Deep-Sea Sponge Geodia barretti

et al. 2021

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Natural product discovery by isolation and structure elucidation is a laborious task often requiring ample quantities of biological starting material and frequently resulting in the rediscovery of previously known compounds. However, peptides are a compound class amenable to an alternative genomic, transcriptomic, and in silico discovery route by similarity searches of known peptide sequences against sequencing data. Based on the sequences of barrettides A and B, we identified five new barrettide sequences (barrettides C–G) predicted from the North Atlantic deep-sea demosponge Geodia barretti (Geodiidae). We synthesized, folded, and investigated one of the newly described barrettides, barrettide C (NVVPCFCVEDETSGAKTCIPDNCDASRGTNP, disulfide connectivity I–IV, II–III). Co-elution experiments of synthetic and sponge-derived barrettide C confirmed its native conformation. NMR spectroscopy and the anti-biofouling activity on larval settlement of the bay barnacle Amphibalanus improvisus (IC 50 0.64 μM) show that barrettide C is highly similar to barrettides A and B in both structure and function. Several lines of evidence suggest that barrettides are produced by the sponge itself and not one of its microbial symbionts.

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Porifera (Sponges)–5

Cited by 2 publications

References 4,431 publications

Oceanographic setting influences the prokaryotic community and metabolome in deep-sea sponges

Oceanographic setting influences the prokaryotic community and metabolome in deep-sea sponges

Barrettides: A Peptide Family Specifically Produced by the Deep-Sea Sponge Geodia barretti

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