Porin self-association enables cell-to-cell contact in
            <i>Providencia stuartii</i>
            floating communities

El-Khatib, Mariam; Nasrallah, Chady; Lopes, Julie; Tran, Que‐Tien; Tetreau, Guillaume; Basbous, Hind; Fenel, Daphna; Gallet, Benoît; Lethier, Mathilde; Bolla, Jean‐Michel; Pagès, Jean‐Marie; Vivaudou, Michel; Weik, Martin H.; Winterhalter, Mathias; Colletier, J.P.

doi:10.1073/pnas.1714582115

Cited by 12 publications

(26 citation statements)

References 28 publications

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“…FCC develop after 4 hours of growth and sediment ~3 hours later (7h) to form SAB. Regardless of the stage of growth or of the socialization mode, we found that Omp-Pst1 is 10-15 fold more expressed than Omp-Pst2, in line with previous results 23 and with the proposition that Omp-Pst1 is the major porin in P. stuartii 22,32 .…”

Section: Resultssupporting

confidence: 92%

“…Recently, we showed that P. stuartii exploits an additional means of socialization before adhesion of cells onto surfaces, whereby self-matching interactions between the extracellular loops of outer membrane (OM) embedded general-diffusion porins enables formation of floating communities of tightly-packed cells (FCC). Formation of FCC precedes that of SAB, suggesting that the latter form from the sedimentation of the former 22,23 .…”

Section: Introductionmentioning

confidence: 94%

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Socialization of Providencia stuartii enables resistance to environmental insults

Lopes

Tetreau

Pounot

et al. 2021

Preprint

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Providencia stuartii is a highly-social pathogen responsible for nosocomial chronic urinary tract infections. The bacterium indeed forms floating communities of cells (FCC) besides and prior-to canonical surface-attached biofilms (SAB). Within P. stuartii FCC, cells are riveted one to another owing to by self-interactions between its porins, viz. Omp-Pst1 and Omp-Pst2. In pathophysiological conditions, P. stuartii is principally exposed to high concentrations of urea, ammonia, bicarbonate, creatinine and to large variations of pH, questioning how these environmental cues affect socialization, and whether formation of SAB and FCC protects cells against those. Results from our investigations indicate that FCC and SAB can both form in the urinary tract, endowing cells with increased resistance and fitness. They additionally show that while Omp-Pst1 is the main gateway allowing penetration of urea, bicarbonate and ammonia into the periplasm, expression of Omp-Pst2 enables resistance to them.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 94%

Socialization of Providencia stuartii enables resistance to environmental insults

Lopes

Tetreau

Pounot

et al. 2021

Preprint

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“…They can also be involved in cell-cell contacts as reported for the Providencia stuartii OmpPst1 12 . In addition, OmpF is required during the entry of colicins and the cellsurface exposed loops of porins are involved in the colicin E3 translocation across the OM 13 .…”

Section: Ompf and Ompc Orthologs Are Present In Closely Related Entermentioning

confidence: 75%

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria

Vergalli¹,

Bodrenko²,

Masi³

et al. 2019

Nat Rev Microbiol

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274

260

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Gram-negative bacteria and their complex cell envelope comprising an outer and inner membrane are an important and attractive system for studying the translocation of small molecules across biological membranes. In the outer membrane of Enterobacteriaceae, trimeric porins control the cellular penetration of small molecules, including nutrients and antibacterial agents. The synergistic action between relatively slow porin-mediated passive uptake across the outer membrane and active efflux transporters in the inner membrane creates a permeability barrier that reinforces the enzymatic modification barrier, which efficiently reduces the intracellular concentrations of small molecules and contributes to the emergence of antibiotic resistance. In this review, we discuss recent advances in our understanding of the molecular and functional roles of classic porins in small molecule translocation in Enterobacteriaceae and consider the crucial role of porins in antibiotic resistance. Commented [w1]: Is this specification necessary here?, in my opinion it deviates, better to put later… Commented [JP2]: Editor request... porins represent the preferred route for the entry of β-lactams, including cephalosporins, penicillins and carbapenems 14-16. The clinical relevance of membrane-associated mechanisms (MAMs) of resistance (i.e. porin defects and/or overexpression of multidrug efflux pumps) has been well established for these antibiotics. The Influx and Efflux rates control the internal concentration of antibiotics and represent the first lane (mechanical barrier) protecting the bacterial cells against therapeutic treatment 1-3,6. Consequently, studies on bacterial porins are receiving a renewed interest due to their key role in the bacterial susceptibility towards clinically used antibiotics. In combination with the expression of antibiotic-modifying enzymes expressed in the periplasm (e.g. β-lactamases), porins play a key role in β-lactam resistance 4,17. In this review, we discuss recent advances in our understanding of the molecular and functional roles of classic porins in antibiotic translocation in Enterobacteriaceae. We explore structural aspects and the insights gained into permeation and the pore translocation process, the regulation of porin expression as well as the role of porins in the emergence of antibiotic susceptibility. Enterobacterial general porins Structural aspects The crystal structures of a general porin from Rhodobacter capsulatus 18 , the OmpF and PhoE porins from E. coli 19 and other E. coli OmpF structures including mutants 20,21 were the first to be solved. Only a limited number of other enterobacterial porin structures have been reported, i.e. E. coli OmpC, K. pneumoniae OmpK36 and Salmonella typhi OmpF 22-24. The lack of data has hindered attempts to relate structure to function. Recently, the structures of two porins from P. stuartii as well as the structures of the OmpF and OmpC orthologs of K. pneumoniae, E. aerogenes and E. cloacae have been reported 12,25,26. Another recent study reported th...

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“…Oligomer formation and stoichiometry characterization. To test the different conditions necessary for obtaining oligomers of Cyt1Aa protoxin and toxin, liposomes were prepared by the standard film-hydration method as previously described 57 . Briefly, liposomes were produced by drying L-α-phosphatidylcholine (POPC; Avanti Polar Lipids, France) under nitrogen flow to obtain a thin lipid film.…”

Section: Methodsmentioning

confidence: 99%

Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

et al. 2020

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Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis (Bti) that has been shown to delay the evolution of insect resistance in the field. Limiting our understanding of Bti efficacy and the path to improved toxicity and spectrum has been ignorance of how Cyt1Aa crystallizes in vivo and of its mechanism of toxicity. Here, we use serial femtosecond crystallography to determine the Cyt1Aa protoxin structure from sub-micron-sized crystals produced in Bti. Structures determined under various pH/redox conditions illuminate the role played by previously uncharacterized disulfidebridge and domain-swapped interfaces from crystal formation in Bti to dissolution in the larval mosquito midgut. Biochemical, toxicological and biophysical methods enable the deconvolution of key steps in the Cyt1Aa bioactivation cascade. We additionally show that the size, shape, production yield, pH sensitivity and toxicity of Cyt1Aa crystals grown in Bti can be controlled by single atom substitution.

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Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities

Cited by 12 publications

References 28 publications

Socialization of Providencia stuartii enables resistance to environmental insults

Socialization of Providencia stuartii enables resistance to environmental insults

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria

Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

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