Porins of Pseudomonas fluorescens MFO as fibronectin-binding proteins

Rebière-Huët, J

doi:10.1016/s0378-1097(02)00920-5

Cited by 29 publications

(13 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using two cellular models, we show here that the decreased toxicity of the oprF mutant is at least partially due to its lowered ability to adhere to cells. This confirms the role of OprF as a cellular adhesin, as previously described using lung epithelial cells (2), fibronectin (58), and wheat root cells, in the case of Pseudomonas fluorescens OE28.3 (20). Furthermore, the reduction of the oprF mutant toxicity is unlikely ascribed only to a lessened affinity to target cells, since a strong decrease was also observed (i) in the production of the exoproducts, namely, pyocyanin, lectin PA-1L, exotoxin A, and elastase, which have cytotoxic properties contributing to tissue damage and to the pathogenesis of P. aeruginosa (36,41,61,77), and (ii) in the activity of T3SS.…”

Section: Discussionsupporting

confidence: 90%

Full Virulence of Pseudomonas aeruginosa Requires OprF

et al. 2011

View full text Add to dashboard Cite

OprF is a general outer membrane porin of Pseudomonas aeruginosa, a well-known human opportunistic pathogen associated with severe hospital-acquired sepsis and chronic lung infections of cystic fibrosis patients. A multiphenotypic approach, based on the comparative study of a wild-type strain of P. aeruginosa, its isogenic oprF mutant, and an oprF-complemented strain, showed that OprF is required for P. aeruginosa virulence. The absence of OprF results in impaired adhesion to animal cells, secretion of ExoT and ExoS toxins through the type III secretion system (T3SS), and production of the quorum-sensing-dependent virulence factors pyocyanin, elastase, lectin PA-1L, and exotoxin A. Accordingly, in the oprF mutant, production of the signal molecules N-(3-oxododecanoyl)-L-homoserine lactone and N-butanoyl-L-homoserine lactone was found to be reduced and delayed, respectively. Pseudomonas quinolone signal (PQS) production was decreased, while its precursor, 4-hydroxy-2-heptylquinoline (HHQ), accumulated in the cells. Taken together, these results show the involvement of OprF in P. aeruginosa virulence, at least partly through modulation of the quorum-sensing network. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production, providing novel insights into virulence expression.

show abstract

Section: Discussionsupporting

confidence: 90%

Full Virulence of Pseudomonas aeruginosa Requires OprF

et al. 2011

View full text Add to dashboard Cite

show abstract

“…As reported for recent proteomic studies, the OprD homologue, which is overexpressed during sessile growth mode, would promote A. baumannii biofilm formation (8,35). It is tempting to consider that this protein might be involved in cell adhesion, as its OprQ counterparts are in P. aeruginosa and P. fluorescens binding to fibronectin (2,49). Associated with its particular affinity for iron, these features could contribute to the involvement of the OprD homologue (which could now be called the OprQ-like protein) in the host colonization process and make it a potential and important virulence factor of A baumannii.…”

Section: Resultsmentioning

confidence: 92%

Deciphering the Function of the Outer Membrane Protein OprD Homologue of Acinetobacter baumannii

Catel-Ferreira

Nehmé

Molle

et al. 2012

Antimicrob Agents Chemother

View full text Add to dashboard Cite

ABSTRACTThe increasing number of carbapenem-resistantAcinetobacter baumanniiisolates is a major cause for concern which restricts therapeutic options to treat severe infections caused by this emerging pathogen. To identify the molecular mechanisms involved in carbapenem resistance, we studied the contribution of an outer membrane protein homologue of thePseudomonas aeruginosaOprD porin. Suspected to be the preferred pathway of carbapenems inA. baumannii, theoprDhomologue gene was inactivated in strain ATCC 17978. Comparison of wild-type and mutant strains did not confirm the expected increased resistance to any antibiotic tested. OprD homologue sequence analysis revealed that this protein actually belongs to an OprD subgroup but is closer to theP. aeruginosaOprQ protein, with which it could share some functions, e.g., allowing bacterial survival under low-iron or -magnesium growth conditions or under poor oxygenation. We thus overexpressed and purified a recombinant OprD homologue protein to further examine its functional properties. As a specific channel, this porin presented rather low single-channel conductance, i.e., 28 pS in 1 M KCl, and was partially closed by micro- and millimolar concentrations of Fe3+and Mg2+, respectively, but not by imipenem and meropenem or basic amino acids. TheA. baumanniiOprD homologue is likely not involved in the carbapenem resistance mechanism, but as an OprQ-like protein, it could contribute to the adaptation of this bacterium to magnesium- and/or iron-depleted environments.

show abstract

“…Fibronectin were pre-coated in sterile 96-well sterile plates as described previously [14]. Briefly, 100 μL fibronectin (Sigma-Aldrich, St. Louis, USA) at 0.1 mg/mL concentration was added to each well overnight, while 20 mg/mL bovine serum albumin (BSA) was added to three wells as blank control wells.…”

Section: Biofilm Production Assaymentioning

confidence: 99%

Rapid transmission of multidrug-resistant Corynebacterium striatum among susceptible patients in a tertiary hospital in China

Wang

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: With increasing reports about Corynebacterium striatum, its potential pathogenicity and clinical significance are drawing more attention in recent years. Methodology: During a 14-month period, Corynebacterium striatum strains were routinely isolated in lower respiratory tract samples of the inpatients in a tertiary hospital in China, and the suspected isolates were identified with VITEK-2 ANC card and 16S rRNA sequencing technique, respectively. Pulsed-field gel electrophoresis (PFGE) was employed to discriminate different clones, and biofilm-producing abilities of different strains were compared. Results: A total of 82 strains of Corynebacterium striatum were mainly isolated from neurosurgery patients (45.1%, 37/82). Three epidemic clones (type D, F, and I) were identified, accounting for 82.9% (68/82) of strains. All 82 C. striatum strains were all sensitive to vancomycin, and resistant to ceftriaxone, imipenem, and ciprofloxacin. One week before C. striatum isolation, 89.0% (73/82) patients showed lower levels of hemoglobin, 93.9% (77/82) of patients received treatment of several kinds of antibiotics, and 41.5% (34/82) patients with glucocorticoid, and 46.3% (38/82) of patients showed disturbed consciousness at different levels. Conclusions: Corynebacterium striatum strains isolated during this study were mainly multidrug resistant, and the predominant clones could rapidly transmit among susceptible inpatients. Corynebacterium striatum seems to be an important pathogen for patients with specific risk factors, especially for the ones with lower hemoglobin levels who are being treated with broad-spectrum antibiotics.

show abstract

Porins of Pseudomonas fluorescens MFO as fibronectin-binding proteins

Cited by 29 publications

References 18 publications

Full Virulence of Pseudomonas aeruginosa Requires OprF

Full Virulence of Pseudomonas aeruginosa Requires OprF

Deciphering the Function of the Outer Membrane Protein OprD Homologue of Acinetobacter baumannii

Rapid transmission of multidrug-resistant Corynebacterium striatum among susceptible patients in a tertiary hospital in China

Contact Info

Product

Resources

About