Porous Au-seeded Ag nanorod networks conjugated with DNA aptamers for impedimetric sensing of DENV-2

De, Sandip Kumar; Ray, Sarmistha; Rawat, Yogita; Mondal, Subrata; Nandy, Arpita; Verma, Priya; Roy, Anuradha; Sadhukhan, Provash Chandra; Das, Chandrima; Bhattacharyya, Sankar; Senapati, Dulal

doi:10.1016/j.snb.2021.130709

Cited by 5 publications

(1 citation statement)

References 43 publications

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“…Preparation of Inactivated Virus Samples. The inactivated dengue virus (DENV) samples were prepared as mentioned before by De et al 34 We have propagated the DENV serotypes in C6/36 cell lines where we used the L15 medium (10% FBS, penicillin−streptomycin) at 28 °C and atmospheric CO 2 . The culture supernatant was collected after 5 days of postinfection and concentrated using a Centricon.…”

Section: ■ Introductionmentioning

confidence: 99%

E-Protein Protonation Titration-Induced Single-Particle Chemical Force Spectroscopy for Microscopic Understanding and pI Estimation of Infectious DENV

Ghosal,

Rakshit,

Bhattacharya

et al. 2024

J. Phys. Chem. B

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The ionization state of amino acids on the outer surface of a virus regulates its physicochemical properties toward the sorbent surface. Serologically different strains of the dengue virus (DENV) show different extents of infectivity depending upon their interactions with a receptor on the host cell. To understand the structural dependence of E-protein protonation over its sequence dependence, we have followed E-protein titration kinetics both experimentally and theoretically for two differentially infected dengue serotypes, namely, DENV-2 and DENV-4. We have performed E-protein protonation titration-induced singleparticle chemical force spectroscopy using an atomic force microscope (AFM) to measure the surface chemistry of DENV in physiological aqueous solutions not only to understand the charge distribution dynamics on the virus surface but also to estimate the isoelectric point (pI) accurately for infectious dengue viruses. Cryo-EM structure-based theoretical pI calculations of the DENV-2 surface protein were shown to be consistent with the evaluated pI value from force spectroscopy measurements. We also highlighted here the role of the microenvironment around the titrable residues (in the 3D-folded structure of the protein) in altering the pK a . This is a comprehensive study to understand how the cumulative charge distribution on the outer surface of a specific serotype of DENV regulates a prominent role of infectivity over minute changes at the genetic level.

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Section: ■ Introductionmentioning

confidence: 99%

E-Protein Protonation Titration-Induced Single-Particle Chemical Force Spectroscopy for Microscopic Understanding and pI Estimation of Infectious DENV

Ghosal,

Rakshit,

Bhattacharya

et al. 2024

J. Phys. Chem. B

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Recent Advances in DNA Nanotechnology‐Based Sensing Platforms for Rapid Virus Detection

Dong,

Guo,

Wang

et al. 2024

ChemBioChem

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Several major viral pandemics in history have significantly impacted the public health of human beings. The COVID‐19 pandemic has further underscored the critical need for early detection and screening of infected individuals. However, current detection techniques are confronted with deficiencies in sensitivity and accuracy, restricting the capability of detecting trace amounts of viruses in human bodies and in the environments.The advent of DNA nanotechnology has opened up a feasible solution for rapid and sensitive virus determination. By harnessing the designability and addressability of DNA nanostructures, a range of rapid virus sensing platforms have been proposed. This review overviewed the recent progress, application, and prospect of DNA nanotechnology‐based rapid virus detection platforms. Furthermore, the challenges and developmental prospects in this field were discussed.

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Porous Au-seeded Ag nanorod networks conjugated with DNA aptamers for impedimetric sensing of DENV-2

Cited by 5 publications

References 43 publications

E-Protein Protonation Titration-Induced Single-Particle Chemical Force Spectroscopy for Microscopic Understanding and pI Estimation of Infectious DENV

E-Protein Protonation Titration-Induced Single-Particle Chemical Force Spectroscopy for Microscopic Understanding and pI Estimation of Infectious DENV

Recent Advances in DNA Nanotechnology‐Based Sensing Platforms for Rapid Virus Detection

Applications of electrochemical impedance spectroscopy in disease diagnosis—A review

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