Porous biocompatible three-dimensional scaffolds of cellulose microfiber/gelatin composites for cell culture

Xing, Qi; Zhao, Feng; Chen, Si; McNamara, James D.; DeCoster, Mark A.; Lvov, Yuri

doi:10.1016/j.actbio.2009.12.036

Cited by 95 publications

(53 citation statements)

References 24 publications

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“…For example, Zhu et al [7] reported that the compressive strength of polyurethane foams was increased and the onset of thermal degradation was delayed when cellulose microfibers were incorporated into the composite foams. Xing et al [8] fabricated the porous biocompatible three-dimensional microscaffolds by mixing the wood cellulose microfibers with cross-linked gelatin. It was found that the cellulose microfiber/gelatin composites can withstand a higher mechanical load compared to the gelatin alone and the bulk cellulose microfiber provides the necessary skeleton in this new scaffold material.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Corncob Cellulose Fibers using Microwave-Assisted Chemical Treatments

Cheng

et al. 2014

International Journal of Food Engineering

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Cellulose fibers were obtained from corncob by using microwave-assisted chemical treatments (microwave-assisted alkaline pretreatment and microwaveassisted bleaching). These treatments efficiently removed the hemicellulose and lignin from the original corncob and increased the cellulose fiber content. The morphology, chemical structure, degree of crystallinity and thermal degradation characteristics of the resultant cellulose fibers were studied by using field emission scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and thermogravimetric analysis. These microwave-assisted chemical treatments decreased the diameter of the cellulose fibers from 25-125 µm to 10-20 µm. The crystallinity of the corncob cellulose fibers increased from 32.7% to 73% due to the chemical treatments. The degradation temperature of the cellulose fibers was > 260°C. The cellulose fibers obtained from these treatments can be used as biocomposites in reinforced polymer manufacturing.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Corncob Cellulose Fibers using Microwave-Assisted Chemical Treatments

Cheng

et al. 2014

International Journal of Food Engineering

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“…Here, the gelatin network formation, gel swelling, gel degradation, water contact angle, and mechanical strength before and after the divalent ion removal were investigated. The cell attachment was also observed by culturing human mesenchymal stem cells (hMSCs), which are easily obtained adult stem cells that show wide and significant use in biomedical applications2021, on top of the gelatin hydrogel. Results showed that the removal of divalent metal ions could significantly enhance the storage and loss moduli as well as the stability of the gelatin hydrogels after chemical crosslinking without affecting the cell attachment on the gelatin hydrogel.…”

mentioning

confidence: 99%

Increasing Mechanical Strength of Gelatin Hydrogels by Divalent Metal Ion Removal

Xing

Yates

Vogt

et al. 2014

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384

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The usage of gelatin hydrogel is limited due to its instability and poor mechanical properties, especially under physiological conditions. Divalent metal ions present in gelatin such as Ca2+ and Fe2+ play important roles in the gelatin molecule interactions. The objective of this study was to determine the impact of divalent ion removal on the stability and mechanical properties of gelatin gels with and without chemical crosslinking. The gelatin solution was purified by Chelex resin to replace divalent metal ions with sodium ions. The gel was then chemically crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Results showed that the removal of divalent metal ions significantly impacted the formation of the gelatin network. The purified gelatin hydrogels had less interactions between gelatin molecules and form larger-pore network which enabled EDC to penetrate and crosslink the gel more efficiently. The crosslinked purified gels showed small swelling ratio, higher crosslinking density and dramatically increased storage and loss moduli. The removal of divalent ions is a simple yet effective method that can significantly improve the stability and strength of gelatin hydrogels. The in vitro cell culture demonstrated that the purified gelatin maintained its ability to support cell attachment and spreading.

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“…14,15 However, the spinning of uniformly sized fibrous structures from the biopolymer and encapsulation of cells in the fiber without damage using a simple method under the mild environment has been challenging. [16][17][18] We and other researchers have suggested the production of alginate or chitosan microfibers using microfluidic chips; however, the microfluidic fabrication of chitosan-alginate fibers has not previously been attempted. A two-step method based on a conventional spinning apparatus ͑first stage: alginate fibers were formed; second stage; fibers were immersed in an acid bath, including partially hydrolyzed chitosan͒ 16,19 was tried to produce chitosanalginate fibers, but this process is complicated, time-consuming, and the uniformity of the distribution of chitosan throughout the alginate fiber has been limited.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic wet spinning of chitosan-alginate microfibers and encapsulation of HepG2 cells in fibers

et al. 2011

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The successful encapsulation of human hepatocellular carcinoma ͑HepG2͒ cells would greatly assist a broad range of applications in tissue engineering. Due to the harsh conditions during standard chitosan fiber fabrication processes, encapsulation of HepG2 cells in chitosan fibers has been challenging. Here, we describe the successful wet-spinning of chitosan-alginate fibers using a coaxial flow microfluidic chip. We determined the optimal mixing conditions for generating chitosanalginate fibers, including a 1:5 ratio of 2% ͑w/w͒ water-soluble chitosan ͑WSC͒ solution to 2% ͑w/w͒ alginate solution. Ratio including higher than 2% ͑w/w͒ WSC solution increased aggregation throughout the mixture. By suspending cells in the WSC-alginate solution, we successfully fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan-alginate fibers were more viable than cells encapsulated in pure alginate fibers, suggesting that cross-linked chitosan provides a better environment for HepG2 cells than alginate alone. In addition, we found that the adhesion of HepG2 cells on the chitosan-alginate fiber is much better than that on the alginate fibers.

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Porous biocompatible three-dimensional scaffolds of cellulose microfiber/gelatin composites for cell culture

Cited by 95 publications

References 24 publications

Isolation and Characterization of Corncob Cellulose Fibers using Microwave-Assisted Chemical Treatments

Isolation and Characterization of Corncob Cellulose Fibers using Microwave-Assisted Chemical Treatments

Increasing Mechanical Strength of Gelatin Hydrogels by Divalent Metal Ion Removal

Microfluidic wet spinning of chitosan-alginate microfibers and encapsulation of HepG2 cells in fibers

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