Porous chitosan/ZnO‐doped bioglass composites as carriers of bioactive peptides

Biernat, Monika; Ciołek, L.; Dzierżyńska, Maria; Oziębło, Artur; Sawicka, Justyna; Deptuła, Milena; Bauer, Marta; Kamysz, Wojciech; Pikuła, Michał; Jaegermann, Z.; Rodziewicz‐Motowidło, Sylwia

doi:10.1111/ijac.13609

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…As an in vitro model, we chose immortalized human cell lines, namely HaCaT keratinocytes and 46BR.1N fibroblasts, which constitute a reliable model for the initial assessment of peptide activity in wound healing. This model was successfully used to evaluate the activity of different peptides, e.g., IM or PDGF peptide derivatives, in our previous works [ 33 , 57 , 58 ]. The analyses performed using the LDH test did not show any cytotoxic effect for any of the tested free peptides or fibrils.…”

Section: Discussionmentioning

confidence: 99%

Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing

Sawicka

Iłowska

Deptuła

et al. 2021

IJMS

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Technological developments in the field of biologically active peptide applications in medicine have increased the need for new methods for peptide delivery. The disadvantage of peptides as drugs is their low biological stability. Recently, great attention has been paid to self-assembling peptides that can form fibrils. Such a formulation makes bioactive peptides more resistant to enzymatic degradation and druggable. Peptide fibrils can be carriers for peptides with interesting biological activities. These features open up prospects for using the peptide fibrils as long-acting drugs and are a valid alternative to conventional peptidic therapies. In our study, we designed new peptide scaffolds that are a hybrid of three interconnected amino acid sequences and are: pro-regenerative, cleavable by neutrophilic elastase, and fibril-forming. We intended to obtain peptides that are stable in the wound environment and that, when applied, would release a biologically active sequence. Our studies showed that the designed hybrid peptides show a high tendency toward regular fibril formation and are able to release the pro-regenerative sequence. Cytotoxicity studies showed that all the designed peptides were safe, did not cause cytotoxic effects and revealed a pro-regenerative potential in human fibroblast and keratinocyte cell lines. In vivo experiments in a dorsal skin injury model in mice indicated that two tested peptides moderately promote tissue repair in their free form. Our research proves that peptide fibrils can be a druggable form and a scaffold for active peptides.

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Section: Discussionmentioning

confidence: 99%

Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing

Sawicka

Iłowska

Deptuła

et al. 2021

IJMS

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“…The sol-gel process allows the attainment of bioactive glass with different compositions and biological properties [48,61]. Furthermore, using the sol-gel technique, bioactive glasses with different porosities can be obtained [62,63].…”

Section: Methods For Obtaining Bioactive Glassmentioning

confidence: 99%

Bioactive Glass—An Extensive Study of the Preparation and Coating Methods

Maximov

Crăciun

et al. 2021

Coatings

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Diseases or complications that are caused by bone tissue damage affect millions of patients every year. Orthopedic and dental implants have become important treatment options for replacing and repairing missing or damaged parts of bones and teeth. In order to use a material in the manufacture of implants, the material must meet several requirements, such as mechanical stability, elasticity, biocompatibility, hydrophilicity, corrosion resistance, and non-toxicity. In the 1970s, a biocompatible glassy material called bioactive glass was discovered. At a later time, several glass materials with similar properties were developed. This material has a big potential to be used in formulating medical devices, but its fragility is an important disadvantage. The use of bioactive glasses in the form of coatings on metal substrates allows the combination of the mechanical hardness of the metal and the biocompatibility of the bioactive glass. In this review, an extensive study of the literature was conducted regarding the preparation methods of bioactive glass and the different techniques of coating on various substrates, such as stainless steel, titanium, and their alloys. Furthermore, the main doping agents that can be used to impart special properties to the bioactive glass coatings are described.

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“…Porous chitosan/bioglass composites were produced by the lyophilization process of stable dispersions chitosan solution and bioglass. The content of chitosan and bioglass in all composites defined as the chitosan/bioglass mass ratio was 1:1 and resulted from the authors' previous experiences [21,22]. With the ratio of components used, the composites were characterized by the highest rigidity and stability of the structure as well as open porosity with pores sizes, appropriated for cell migration and proliferation.…”

Section: Preparation Conditions Of Stable Porous Chitosan/bioglass Co...mentioning

confidence: 99%

“…For crosslinking/stabilizing chitosan porous structures in the form of scaffolds, genipin [10][11][12][13], L-aspartic acid [14,15], vanillin [16], sodium carbonate [17,18], sodium alginate [19], ethanol [17,[19][20][21][22], thermal dehydration [23,24], and sodium tripolyphosphate [16,25,26] have been described previously. All these methods (except genipin) are inexpensive and simple and do not require the use of catalysts.…”

Section: Introductionmentioning

confidence: 99%

Effect of Selected Crosslinking and Stabilization Methods on the Properties of Porous Chitosan Composites Dedicated for Medical Applications

Biernat

Woźniak

Chraniuk³

et al. 2023

Polymers

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Chitosan is one of the most commonly employed natural polymers for biomedical applications. However, in order to obtain stable chitosan biomaterials with appropriate strength properties, it is necessary to subject it to crosslinking or stabilization. Composites based on chitosan and bioglass were prepared using the lyophilization method. In the experimental design, six different methods were used to obtain stable, porous chitosan/bioglass biocomposite materials. This study compared the crosslinking/stabilization of chitosan/bioglass composites with ethanol, thermal dehydration, sodium tripolyphosphate, vanillin, genipin, and sodium β-glycerophosphate. The physicochemical, mechanical, and biological properties of the obtained materials were compared. The results showed that all the selected crosslinking methods allow the production of stable, non-cytotoxic porous composites of chitosan/bioglass. The composite with genipin stood out with the best of the compared properties, taking into account biological and mechanical characteristics. The composite stabilized with ethanol is distinct in terms of its thermal properties and swelling stability, and it also promotes cell proliferation. Regarding the specific surface area, the highest value exposes the composite stabilized by the thermal dehydration method.

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Porous chitosan/ZnO‐doped bioglass composites as carriers of bioactive peptides

Cited by 10 publications

References 56 publications

Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing

Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing

Bioactive Glass—An Extensive Study of the Preparation and Coating Methods

Effect of Selected Crosslinking and Stabilization Methods on the Properties of Porous Chitosan Composites Dedicated for Medical Applications

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