Porous decellularized adipose tissue foams for soft tissue regeneration

Yu, Claire; Bianco, Juares; Brown, Cody; Fuetterer, Lydia; Watkins, John; Samani, Abbas; Flynn, Lauren E.

doi:10.1016/j.biomaterials.2013.01.056

Cited by 154 publications

(174 citation statements)

References 44 publications

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“…The level of expression of PPARγ, CEBPα, and LPL genes was higher in the tissue-printed construct than in DAT gel at all-time points; this difference indicates that the 3D constructs nurture the embedded cells better than that does the gel (Fig. 4a), possibly because the 3D open porous structure fabricated using our tissue printing method provides efficient transfer of nutrition and oxygen to the embedded cells [18]. The adipo-inductive nature of the DAT gel has been reported previously [14]; nevertheless, here we show that by fabricating a 3D structure with engineered porosity using DAT, the level of induction of adipogenesis and functionality can be enhanced further.…”

Section: Evaluation Of Differentiation Into Adipogenic Lineagementioning

confidence: 79%

“…It was reported that pore sizes greater than 100 mm are required to support extensive cellular infiltration, to allow for diffusion of oxygen and nutrients, and to accommodate the expansion of maturing adipocytes following in vivo implantation [18]. Various strategies has been used to overcome this limitation by either making porous microscarriers [58] or foams [18]. However, the mechanical properties of these porous structures were typically low and cannot sustain the tissue regeneration when implanted.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

“…Tissue decellularization involving a combination of physical, chemical, and biological treatment is one strategy that has been considered as an effective method for fabrication of ECM scaffolds [15]. However, the ECM materials harvested and processed from tissues often lack a tailored microgeometry [17] and thus cellular distribution is mainly limited to the surface of ECM scaffolds, with only a fraction of total cells penetrating into the interior regions [18]. Furthermore, when ECM scaffolds are implanted, survival of the infiltrating or seeded cell populations is supported primarily by diffusion of oxygen and nutrients until a supporting vascular network forms [19].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

“…Decellularized adipose tissue has been used as scaffolds for cell seeding and tissue engineering [14,18]. However, cellular infiltration was the main challenge for populating these scaffolds with homogeneous distribution of cells.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

“…However, cellular infiltration was the main challenge for populating these scaffolds with homogeneous distribution of cells. It was reported that pore sizes greater than 100 mm are required to support extensive cellular infiltration, to allow for diffusion of oxygen and nutrients, and to accommodate the expansion of maturing adipocytes following in vivo implantation [18]. Various strategies has been used to overcome this limitation by either making porous microscarriers [58] or foams [18].…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

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Biomimetic 3D tissue printing for soft tissue regeneration

et al. 2015

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Section: Evaluation Of Differentiation Into Adipogenic Lineagementioning

confidence: 79%

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

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Biomimetic 3D tissue printing for soft tissue regeneration

et al. 2015

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Characterization and Proteomic Analysis of Decellularized Adipose Tissue Hydrogels Derived from Lean and Overweight/Obese Human Donors

et al. 2020

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While decellularized adipose tissue (DAT) has potential as an “off‐the‐shelf” biomaterial product for regenerative medicine, it remains to be determined if donor‐source body mass index (BMI) impacts the functionality of DAT. This study set out to comparatively characterize lean versus overweight/obese‐donor derived DAT hydrogel based on proteome and to analyze their respective effects on adipose stromal/stem cell (ASC) viability, and differentiation in vitro. Decellularized adipose tissue from lean (lDAT) and overweight/obese (oDAT) donors is produced and characterized. Variability in the fibril microstructures is found, with dense fibrotic fiber clusters and large pore area uniquely present in the oDAT samples. Proteomic analysis reveals that lDAT contains a greater proportion of enriched extracellular proteins and a smaller proportion of enriched intracellular proteins relative to oDAT. Biocompatibility studies show that ASCs cultured in lDAT and oDAT hydrogels remain viable. The adipogenic and osteogenic differentiation capability of ASCs seeded in lDAT and oDAT hydrogels is confirmed by an upregulation in marker gene expression and phenotypic analysis. In conclusion, this study establishes that DAT hydrogels derived from lean and overweight/obese adipose donors present similar physicochemical profiles with some distinctive features while comparably supporting the viability and adipogenic differentiation of ASCs in vitro.

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Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Krishtul

Baruch

Machluf

2019

Adv Funct Materials

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Tissue-derived decellularized extracellular matrices (dECM) have gradually become the gold standard of scaffolds for tissue engineering, owing to their close mirroring of the intricate composition, architecture, and topology of the native extracellular matrix (ECM). Intriguingly, further manipulation of these acellular tissues through various processing techniques has been demonstrated to be an effective strategy to control their characteristics and impart them with ample valuable new traits, thereby expanding their applicability to a significantly wider spectrum of research and translational applications. Herein, state-of-the-art processed dECM platforms and their potential applications are focused on. The ECM characteristics that make it so appealing for tissue engineering are presented, followed by a concise discussion on the main considerations for choosing a dECM source for such applications. The key methodologies for dECM processing, including hydrogel production, bioprinting, electrospinning, and production of porous scaffolds, microcarriers, and microcapsules, as well as their inherent advantages and challenges, are introduced. To demonstrate the use of processed dECM platforms for tissue engineering, selected in vivo and in vitro applications recently developed utilizing these platforms are highlighted. Finally, concluding remarks and a prospective outlook for future developments and improvements in the field of processed dECM-based devices are given.

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Porous decellularized adipose tissue foams for soft tissue regeneration

Cited by 154 publications

References 44 publications

Biomimetic 3D tissue printing for soft tissue regeneration

Biomimetic 3D tissue printing for soft tissue regeneration

Characterization and Proteomic Analysis of Decellularized Adipose Tissue Hydrogels Derived from Lean and Overweight/Obese Human Donors

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

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