Porous hydrogel biomedical foam scaffolds for tissue repair

Vlierberghe, Sandra Van; Graulus, Geert-Jan; Samal, Sangram Keshari; Nieuwenhove, Ine Van; Dubruel, Peter

doi:10.1533/9780857097033.2.335

Cited by 55 publications

(45 citation statements)

References 211 publications

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“…Due to the presentation of Arg-Gly-Asp peptide sequences, gelatin can promote cell adhesion [44]. Also, gelatin is a positive-charged at physiological condition [45]. These reasons could lead to a highly attractive interaction to normal or cancer cells.…”

Section: Resultsmentioning

confidence: 99%

Dual Interactions of Amphiphilic Gelatin Copolymer and Nanocurcumin Improving the Delivery Efficiency of the Nanogels

et al. 2019

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Herein, a new process to manufacture multicore micelles nanoparticles reinforced with co-assembly via hydrophobic interaction and electrostatic interaction under the help of ultrasonication was developed. The precise co-assembly between negative/hydrophobic drug and positive charged amphiphilic copolymer based pluronic platform allows the formation of complex micelles structures as the multicore motif with predefined functions. In this study, curcumin was selected as a drug model while positively charged copolymer was based on a pluronic-conjugated gelatin with different hydrophobicity length of Pluronic F87 and Pluronic F127. Under impact of dual hydrophobic and electrostatic interactions, the nCur-encapsulated core–shell micelles formed ranging from 40 nm to 70 nm and 40–100 nm by transmission electron microscopy (TEM) and Dynamic Light Scattering (DLS), respectively. It is found that the structures emerged depended on the relative lengths of the hydrophobic blocks in pluronic. Regarding g2(τ) behavior from DLS measurement, the nanogels showed a high stability in spherical form. Surprisingly, the release profiles showed a sustainable behavior of Cur from this system for drug delivery approaches. In vitro study exhibited that nCur-encapsulated complex micelles increased inhibitory activity against cancer cells growth with IC50 is 4.02 ± 0.11 mg/L (10.92 ± 0.3 µM) which is higher than of free curcumin at 9.40 ± 0.17 mg/L (25.54 ± 0.18 µM). The results obtained can provide the new method to generate the hierarchical assembly of copolymers with incorporated loading with the same property.

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Section: Resultsmentioning

confidence: 99%

Dual Interactions of Amphiphilic Gelatin Copolymer and Nanocurcumin Improving the Delivery Efficiency of the Nanogels

et al. 2019

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“…It is a natural product of animal origin from which two types are discerned, depending on tissue pre-treatment. Acid-cured tissue renders type A gelatine and with alkaline pre-treatment type B gelatine is obtained [8]. Gelatine is the base material for the manufacturing of gelatine methacryloyl (GelMA), first described by Den Bulcke et al (2000).…”

Section: Introductionmentioning

confidence: 99%

Impact of Endotoxins in Gelatine Hydrogels on Chondrogenic Differentiation and Inflammatory Cytokine Secretion In Vitro

Groen

Utomo

Castilho

et al. 2020

IJMS

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Gelatine methacryloyl (GelMA) hydrogels are widely used in studies aimed at cartilage regeneration. However, the endotoxin content of commercially available GelMAs and gelatines used in these studies is often overlooked, even though endotoxins may influence several cellular functions. Moreover, regulations for clinical use of biomaterials dictate a stringent endotoxin limit. We determined the endotoxin level of five different GelMAs and evaluated the effect on the chondrogenic differentiation of equine mesenchymal stromal cells (MSCs). Cartilage-like matrix production was evaluated by biochemical assays and immunohistochemistry. Furthermore, equine peripheral blood mononuclear cells (PBMCs) were cultured on the hydrogels for 24 h, followed by the assessment of tumour necrosis factor (TNF)-α and C–C motif chemokine ligand (CCL)2 as inflammatory markers. The GelMAs were found to have widely varying endotoxin content (two with >1000 EU/mL and three with <10 EU/mL), however, this was not a critical factor determining in vitro cartilage-like matrix production of embedded MSCs. PBMCs did produce significantly higher TNF-α and CCL2 in response to the GelMA with the highest endotoxin level compared to the other GelMAs. Although limited effects on chondrogenic differentiation were found in this study, caution with the use of commercial hydrogels is warranted in the translation from in vitro to in vivo studies because of regulatory constraints and potential inflammatory effects of the content of these hydrogels.

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“…Two types of gelatin are generally available, depending on the pretreatment procedure (before the extraction process). Acidic pretreatment (type A) hardly affects the amide group, while alkaline pretreatment (type B) targets the amide groups of asparagine and glutamine and hydrolyzes them to carboxyl groups, thus converting many of these residues for aspartic acid and glutamic acid [91]. At the isoelectric point (IEP), gelatin has a neutral net charge as a balance between the positive charge from the NH3 + ion and the negative charge from the COOion.…”

Section: Research On the Electrospinning Of Gelatine Compositesmentioning

confidence: 99%

Advances in Electrospinning of Natural Biomaterials for Wound Dressing

Fa-dong

Jia

et al. 2020

Journal of Nanomaterials

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Electrospinning has been recognized as an efficient technique for the fabrication of polymer nanofibers. Various polymers have been successfully electrospun into ultrafine fibers in recent years. These electrospun biopolymer nanofibers have potential applications for wound dressing based upon their unique properties. In this paper, a comprehensive review is presented on the researches and developments related to electrospun biopolymer nanofibers including processing, structure and property, characterization, and applications. Information of those polymers together with their processing condition for electrospinning of ultrafine fibers has been summarized in the paper. The application of electrospun natural biopolymer fibers in wound dressings was specifically discussed. Other issues regarding the technology limitations, research challenges, and future trends are also discussed.

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Porous hydrogel biomedical foam scaffolds for tissue repair

Cited by 55 publications

References 211 publications

Dual Interactions of Amphiphilic Gelatin Copolymer and Nanocurcumin Improving the Delivery Efficiency of the Nanogels

Dual Interactions of Amphiphilic Gelatin Copolymer and Nanocurcumin Improving the Delivery Efficiency of the Nanogels

Impact of Endotoxins in Gelatine Hydrogels on Chondrogenic Differentiation and Inflammatory Cytokine Secretion In Vitro

Advances in Electrospinning of Natural Biomaterials for Wound Dressing

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