Porous organosilicone modified gelatin hybrids with controllable and homogeneous in vitro degradation behaviors for potential application as skin regeneration scaffold

Abstract

This work aims at investigating intensively the effects of organosilicone species and their dosage on the physicochemical and particularly the in vitro degradation properties of gelatin hybrids. We prepared various porous organosilicone modified gelatin hybrids with epoxy-polydimethylsiloxane (PDMS) and/or glycidoxypropyltrimethoxysilane (GPTMS) and further systematically investigated their degradation behaviors in simulated physiological environments. It was found that the chemical composition, thermal stability, crosslinking degree, mechanical properties and porous structure of the gelatin hybrids could be tuned by adjusting the amount of PDMS and GPTMS. More importantly, degradation rates of the gelatin hybrids were reduced with increasing content of GPTMS, implying that the degradation behaviors could be controlled by tailoring the chemical interaction between the gelatin and organosilicone moieties. In addition, gelatin hybrids modified with both PDMS and GPTMS (PGs-GE) were demonstrated as a homogeneous hybridization, and their maximum weight losses met the typical healing period of a normal skin wound. Noticeably, the P1G1-GE hybrid with PDMS to GPTMS molar ratio 1:1 exhibited appropriate weight loss, integrity of pore structure and synchronous dissolution of silicon and protein during the degradation process, indicating a homogeneous degradation behavior. Furthermore, both the original and degraded P1G1-GE hybrid exhibited favorable cytocompatibility in vitro. The findings will be helpful for further insight into the in vivo degradation of gelatin hybrids, suggesting their potential application as skin regeneration scaffolds.

Superhydrophobic tough hierarchical porous thermal insulation composites prepared by in situ formation of silica aerogel in collagen fiber matrix

“…And the asymmetric stretching vibration of CH 2 appeared at 2950 cm −1 , while peaks at 1634, 1538, and 1450 cm −1 are corresponding to CO stretching vibration, NH bending vibration (amide II), and CH bending vibration, respectively 28,29 . In addition, from the FT‐IR spectrum of SiO 2 aerogel, it can be seen that the peak appeared at 2950 cm −1 is the characteristic peak of CH, and the peak around 1253 cm −1 is the stretching vibration of SiC, indicating that SiCH 3 is successfully grafted to the surface of the silica aerogel by HMDS 30,31 . The peak around 1084 cm −1 can be attributed to the stretching vibration of SiOSi bond, and the peak at 950 cm −1 can be attributed to the bending vibration of the SiOH group.…”

Preparation of PEG‐modified wool keratin/sodium alginate porous scaffolds with elasticity recovery and good biocompatibility

“…), although have excellent mechanical properties, their bioactivity, and antiinfection ability are poor. Porous materials prepared from natural polymers (chitosan, 8 gelatin, 9 etc.) have good bioactivity, but most of them have poor mechanical properties or few raw materials, which are difficult to be widely used.…”

Influence of the weight ratio of polydimethylsiloxane modified gelatin to silicone rubber on the potential performance of asymmetric bilayer membranes as wound dressings

“…However, poor mechanical strength, high hydrophilicity and rapid degradability greatly limit its effective and wide application . Our previous studies demonstrated that the incorporation of polydimethylsiloxane (PDMS) chains in GE molecules could improve the mechanical flexibility, porous structure and degradation resistance, but the proposed GE‐based materials are simply designed as a single‐layer film . In contrast, silicone rubber (SR), a typical synthetic material, has inherent hydrophobicity, high flexibility and good biocompatibility but poor biodegradability .…”

“…15,16 Our previous studies demonstrated that the incorporation of polydimethylsiloxane (PDMS) chains in GE molecules could improve the mechanical flexibility, porous structure and degradation resistance, but the proposed GE-based materials are simply designed as a single-layer www.soci.org W Du, Z Zhang, Z Li film. [17][18][19] In contrast, silicone rubber (SR), a typical synthetic material, has inherent hydrophobicity, high flexibility and good biocompatibility but poor biodegradability. 20,21 As known from the self-stratification strategy, the combination of GE or modified GE and SR is a feasible approach to fabricate a porous bilayer membrane, thus expressing their respective advantages.…”