Porous Poly(3-hydroxybutyrate) Scaffolds Prepared by Non-Solvent-Induced Phase Separation for Tissue Engineering

Kang, Jiseon; Hwang, Ji-Young; Huh, Mongyoung; Yun, Seok Il

doi:10.1007/s13233-020-8109-x

Cited by 22 publications

(13 citation statements)

References 53 publications

(93 reference statements)

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“…In the end, the in vivo osteogenesis and the vascular formation were significantly promoted in scaffolds with DFO loading. In Kang’s study, highly porous poly(3-hydroxybutyrate) (PHB) scaffolds were fabricated using nonsolvent-induced phase separation followed by freeze-drying under 30 mTorr at −130 °C for 2 days [ 21 ]. The scaffolds showed well-connected micropores with increasing nonsolvent content and porosity in the range of 97 to 99%.…”

Section: Combination Of Solution-based Techniquesmentioning

confidence: 99%

“…Generally, the choice of the material for solution-based technologies relies on the desired morphology, function, and application of the produced scaffold, including biopolymers such as polycaprolactone (PCL) [ 9 , 10 ], polylactide (PLA) [ 11 , 12 , 13 ], poly(lactide-co-glycolide; PLGA) [ 14 ], poly(vinyl) alcohol (PVA) [ 15 ], gelatin [ 16 ], chitosan [ 10 ], and collagen [ 17 , 18 ]. According to the expected structural and mechanical performance for tissue replacement, different solution-based fabrication methods could be applied ( Figure 1 ), such as freeze-drying [ 17 , 19 , 20 ] ( Figure 1 a), Thermally or Diffusion Induced Phase Separation (TIPS ( Figure 1 b) or DIPS ( Figure 1 c)) [ 21 , 22 ], electrospinning [ 23 , 24 ] ( Figure 1 d), or a valuable combination of them [ 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

et al. 2021

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The fabrication of 3D scaffolds is under wide investigation in tissue engineering (TE) because of its incessant development of new advanced technologies and the improvement of traditional processes. Currently, scientific and clinical research focuses on scaffold characterization to restore the function of missing or damaged tissues. A key for suitable scaffold production is the guarantee of an interconnected porous structure that allows the cells to grow as in native tissue. The fabrication techniques should meet the appropriate requirements, including feasible reproducibility and time- and cost-effective assets. This is necessary for easy processability, which is associated with the large range of biomaterials supporting the use of fabrication technologies. This paper presents a review of scaffold fabrication methods starting from polymer solutions that provide highly porous structures under controlled process parameters. In this review, general information of solution-based technologies, including freeze-drying, thermally or diffusion induced phase separation (TIPS or DIPS), and electrospinning, are presented, along with an overview of their technological strategies and applications. Furthermore, the differences in the fabricated constructs in terms of pore size and distribution, porosity, morphology, and mechanical and biological properties, are clarified and critically reviewed. Then, the combination of these techniques for obtaining scaffolds is described, offering the advantages of mimicking the unique architecture of tissues and organs that are intrinsically difficult to design.

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Section: Combination Of Solution-based Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

et al. 2021

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“…Furthermore, in similar applications for skin engineering, the fiber matrices based on PHBV and obtained by electrospinning demonstrated that human skin fibroblasts (CRL 2072) were able to adhere and colonize these new substrates [44]. Different PHB organogels and scaffolds with complex hierarchical structure and covering a wide range of length scales have been prepared by TIPS and showed an excellent cell viability using the human keratinocyte cell line (HaCaT) [46]. Finally, our results contribute to the suggestion of the great application of PHBV in tissue engineering.…”

Section: Biocompatibility Of Porous Phbv Scaffoldsmentioning

confidence: 99%

Poly(hydroxybutyrate-co-hydroxyvalerate) Porous Matrices from Thermally Induced Phase Separation

et al. 2020

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Thermally induced phase separation followed by freeze drying has been used to prepare biodegradable and biocompatible scaffolds with interconnected 3D microporous structures from poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) copolymers containing 5 and 12 wt % of 3-hydroxyvalerate (HV). Solutions of PHBV in 1,4-dioxane, underwent phase separation by cooling under two different thermal gradients (at −25 °C and −5 °C). The cloud point and crystallization temperature of the polymer solutions were determined by turbidimetry and differential scanning calorimetry, respectively. Parameters affecting the phase separation mechanism such as variation of both the cooling process and the composition of the PHBV copolymer were investigated. Afterwards, the influence of these variables on the morphology of the porous structure and the final mechanical properties (i.e., rigidity and damping) was evaluated via scanning electron microscopy and dynamic mechanical thermal analysis, respectively. While the morphology of the scaffolds was considerably affected by polymer crystallization upon a slow cooling rate, the effect of solvent crystallization was more evident at either high hydroxyvalerate content (i.e., 12 wt % of HV) or high cooling rate. The decrease in the HV content gave rise to scaffolds with greater stiffness because of their higher degree of crystallinity, being also noticeable the greater consistency of the structure attained when the cooling rate was higher. Scaffolds were fully biocompatible supports for cell adhesion and proliferation in 3D cultures and show potential application as a tool for tissue regeneration.

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“…Biomimetic cellular environments can be produced from the synthesis of polyhydroxyalkanoate (PHA) scaffolds (1). Poly (3-hydroxybutyrate) [PHB] is a PHA frequently used for producing new biocompatible materials, with many physicochemical characteristics relevant for biomedical applications, and it has been combined with diverse materials to prepare scaffolds that support cell proliferation (2)(3)(4). Due to its excellent biodegradability and biocompatibility properties, chitosan (CS) grafted (g) onto PHB appears to be a viable option to produce scaffolds that maintain cell growth (5,6).…”

Section: Introductionmentioning

confidence: 99%

A poly (saccharide-ester-urethane) scaffold for mammalian cell growth

González‐Torres

Becerra-González

Leyva‐Gómez

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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Chitosan and poly(3-hydroxybutyrate) are non-toxic, biodegradable, and biocompatible polymers extensively used in regenerative medicine. However, it is unknown whether the chemical combination of these polymers can produce a biomaterial that induces an appropriate cellular response in vitro in mammalian cells. This study aimed to test the ability of a novel salt-leached polyurethane scaffold of chitosan grafted with poly(3-hydroxybutyrate) to support the growth of three mammalian cell lines of different origin: a) HEK-293 cells, b) i28 mouse myoblasts, and c) human dermal fibroblasts. The viability of the cells was assessed by either evaluation of their capacity to maintain the expression of the green fluorescent protein by adenoviral transduction or by esterase activity and plasma membrane integrity. The results indicated that the three cell lines attached well to the scaffold; however, when i28 cells were induced to differentiate, they did not produce morphologically distinct myofibers, and cell growth ceased. In conclusion, the findings reveal that, altogether, these observations suggest that this foam scaffold supports cell growth and proliferation but may not apply to all cell types. Hence, one crucial question yet to be resolved is a poly (saccharide-ester-urethane) derivative with a nano-topography that elicits a similar cellular response for different biological environments.

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Porous Poly(3-hydroxybutyrate) Scaffolds Prepared by Non-Solvent-Induced Phase Separation for Tissue Engineering

Cited by 22 publications

References 53 publications

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

Poly(hydroxybutyrate-co-hydroxyvalerate) Porous Matrices from Thermally Induced Phase Separation

A poly (saccharide-ester-urethane) scaffold for mammalian cell growth

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