“…Each scaffold was exposed to UV radiation for both top and bottom surfaces in a laminar flow hood (each side for 30 min), washed 3 times with PBS for 20 min and incubated with medium culture for 24 h before cell seeding. The morphology of grown PC12 cells on nanofibrous scaffolds was observed by SEM as described by Nasri-Nasrabadi et al [28]. After 1, 4, and 7 days of cell seeding, the cells were fixed by glutaraldehyde solution (4%, Merck) in PBS for 24 h at 4 • C. The dehydration process was performed on each specimen in a series of graded alcohols (10%, 30%, 50%, 70%, 80%, 85%, 90%, 95%, and 100%), each for 10 min.…”