Porphyrins in Erythrocytes and Blood Plasma: Preliminary Studies with Qualitative Thin-Layer Chromatography

With, Torben K.

doi:10.1042/cs0520463

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…In Figure 3B, it is demonstrated that Ce 6 generates 1 O 2 very quickly, compared to Au NCs, however, the maximum amount of 1 O 2 is very similar to the amount which is generated by Au NCs after longer irradiation. Additionally, we observed that human plasma itself also generates 1 O 2 upon light exposure, which could be easily explained by the presence of endogenous photosensitizers, such as uroporphyrin, coproporphyrin, and protoporphyrin, in plasma [54]. Thus, our studies have demonstrated that Au NCs stabilized with plasma proteins can generate ROS under 405 nm light irradiation.…”

Section: Singlet Oxygen and Other Reactive Oxygen Species Generationsupporting

confidence: 50%

Blood Plasma Stabilized Gold Nanoclusters for Personalized Tumor Theranostics

Jarockytė

Poderys

Barzda

et al. 2022

Cancers

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Personalized cancer theranostics has a potential to increase efficiency of early cancer diagnostics and treatment, and to reduce negative side-effects. Protein-stabilized gold nanoclusters may serve as theranostic agents. To make gold nanoclusters personalized and highly biocompatible, the clusters were stabilized with human plasma proteins. Optical properties of synthesized nanoclusters were investigated spectroscopically, and possible biomedical application was evaluated using standard cell biology methods. The spectroscopic investigations of human plasma proteins stabilized gold nanoclusters revealed that a wide photoluminescence band in the optical tissue window is suitable for cancer diagnostics. High-capacity generation of singlet oxygen and other reactive oxygen species was also observed. Furthermore, the cluster accumulation in cancer cells and the photodynamic effect were evaluated. The results demonstrate that plasma proteins stabilized gold nanoclusters that accumulate in breast cancer cells and are non-toxic in the dark, while appear phototoxic under irradiation with visible light. The results positively confirm the utility of plasma protein stabilized gold nanoclusters for the use in cancer diagnostics and treatment.

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Section: Singlet Oxygen and Other Reactive Oxygen Species Generationsupporting

confidence: 50%

Blood Plasma Stabilized Gold Nanoclusters for Personalized Tumor Theranostics

Jarockytė

Poderys

Barzda

et al. 2022

Cancers

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Coproporphyrinogen oxidase activity and porphyrin concentrations in peripheral red blood cells in hereditary sideroblastic anaemia

Pasanen

EklöUf

Tenhunen

1985

Scandinavian Journal of Haematology

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The activity of coproporphyrinogen oxidase and the concentrations of coproporphyrin and protoporphyrin (measured by HPLC) in peripheral red blood cells were established in 2 families with different types of hereditary sideroblastic anaemia. 2 males and 4 females were members of a family with an X‐chromosome‐linked and pyridoxine‐responsive HSA, and 3 females were members of another family where the mode of inheritance is not clear and where pyridoxine did not produce a haemotological response. Coproporphyrinogen oxidase activity was normal in 8 of 9 patients and slightly decreased only in 1 patient. All patients had normal red cell coproporphyrin concentrations, but red cell protoporphyrin concentration was decreased in 4 patients. These findings indicate that in vivo haem synthesis was not impaired at the step of coproporphyrinogen oxidase, hence enzymatic defects in earlier steps of haem synthesis are more evident. Earlier suggestions of impaired haem synthesis at this level, based on observed increased concentrations of coproporphyrin in peripheral red blood cells might be explained by the use of unspecific methods.

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Relaxation of rat vascular muscle by peripheral benzodiazepine modulators.

Erné¹,

Chiesi²,

Longoni³

et al. 1989

J. Clin. Invest.

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Effects of peripheral benzodiazepine receptor modulating drugs, Ro 5-4864 and PK 11195, on tension induced by K+ and the calcium agonist SDZ 202 791 (S isomer), were studied in rat caudal arteries. A significant reduction of tonic phase tension occurred with 30 nM PK 11195 or 3 IAM Ro 5-4864, but decreases of the initial (first 3 min), phasic contraction were detected only at the highest concentrations of Ro 5-4864 and PK 11195. Protoporphyrin IX, the putative endogenous ligand of the peripheral benzodiazepine receptor, (at 10-100 nM) markedly increased the effectiveness of Ro 5-4864 and PK 11195 in reducing phasic contraction. Intracellular calcium localization and distribution in fura-2 loaded single vascular cells were quantitated using a high sensitivity, two-stage microchannel plate, photon-counting (PMI-VIM) camera. Peripheral benzodiazepines reduced intracellular calcium release from centrally located calcium pools, and this decrease of calcium release was potentiated by protoporphyrin IX. The decrease in intracellular calcium activity, which was more pronounced in the central regions where sarcoplasmic reticular elements are numerous, was probably the major mechanism of these vasodilator properties.Measurements of soluble guanylate cyclase activity also supported the intracellular Ca2" release mechanism. Under conditions where protoporphyrin IX did not significantly stimulate guanylate cyclase, Ro 54864 alone or more effectively in combination with protoporphyrin IX stimulated cGMP production and caused relaxation. Guanylate cyclase forms a possible target for these benzodiazepine modulators, a hypothesis that merits further investigation.

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Porphyrins in Erythrocytes and Blood Plasma: Preliminary Studies with Qualitative Thin-Layer Chromatography

Cited by 3 publications

References 6 publications

Blood Plasma Stabilized Gold Nanoclusters for Personalized Tumor Theranostics

Blood Plasma Stabilized Gold Nanoclusters for Personalized Tumor Theranostics

Coproporphyrinogen oxidase activity and porphyrin concentrations in peripheral red blood cells in hereditary sideroblastic anaemia

Relaxation of rat vascular muscle by peripheral benzodiazepine modulators.

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