Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine

Porphyromonas gingivalis Suppresses Trophoblast Invasion by Soluble Factors

“…However, it is difficult to explain the increased frequency of PE in patients with periodontitis. In a previous study, the authors reported that Pg lipopolysaccharide suppressed trophoblastic invasion in the presence of nicotine, 33 and results are consistent with pregnancy complications that are common among smokers. However, a recent report suggested that periodontitis is an independent risk factor for PE in non‐smoking women 34 .…”

“…In a previous study, the authors reported suppression of trophoblast invasion by Pg lipopolysaccharide only in the presence of nicotine. 33 The results may reasonably explain the frequent pregnancy complications among smokers, although a recent report suggested that periodontitis is an independent risk factor for PE in non-smoking women. 34 In this study, it was revealed that other factors produced by Pg are responsible for suppression of trophoblast invasion.…”

“…Bacterial products from both oral and placental sites may affect placental functions. In a previous study, the authors reported suppression of trophoblast invasion by Pg lipopolysaccharide only in the presence of nicotine 33 . The results may reasonably explain the frequent pregnancy complications among smokers, although a recent report suggested that periodontitis is an independent risk factor for PE in non‐smoking women 34 .…”

“…To examine effects of soluble components derived from Pg for HTR‐8/SVneo cells, cell viability was evaluated with a formazan formation assay using a cell counting kit 33 ‖‖ . Briefly, HTR‐8/SVneo cells were plated in a 96‐well plate at 1 × 10 4 cells per well.…”

“…Gelatinous protein mixture invasion assays were performed as previously described 33 ## **** ††† ‡‡‡ . For estimation of invasion, 5 × 10 4 cells were plated in 500 μL culture medium in the camber on top of the solubilized extracellular matrix rich (ECM) membrane preparation with or without serial dilutions of PG‐sup for 24 hours.…”