Portable aptamer biosensor of platelet-derived growth factor-BB using a personal glucose meter with triply amplified

Hong, Lü; Zhou, Fu; Shi, Dongmin; Zhang, Xiaojun; Wang, Guangfeng

doi:10.1016/j.bios.2017.04.023

Cited by 49 publications

(10 citation statements)

References 52 publications

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“…Besides monitoring environmental contamination described above, examples of the recently developed biosensors include the following: (1) Aptamer-based biosensors to target disease biomarkers, such as platelet-derived growth factor BB (PDGF-BB), a cancer related protein, to help early diagnosis and prognosis of cancer development [134]. (2) Biosensors for the azole class of antifungal drugs with application in the therapeutic drug monitoring of patients with invasive fungal infections [135].…”

Section: Applications Of Aptamersmentioning

confidence: 99%

Recent Advances in Aptamer Discovery and Applications

2019

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Aptamers are short, single-stranded DNA, RNA, or synthetic XNA molecules that can be developed with high affinity and specificity to interact with any desired targets. They have been widely used in facilitating discoveries in basic research, ensuring food safety and monitoring the environment. Furthermore, aptamers play promising roles as clinical diagnostics and therapeutic agents. This review provides update on the recent advances in this rapidly progressing field of research with particular emphasis on generation of aptamers and their applications in biosensing, biotechnology and medicine. The limitations and future directions of aptamers in target specific delivery and real-time detection are also discussed.

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Section: Applications Of Aptamersmentioning

confidence: 99%

Recent Advances in Aptamer Discovery and Applications

2019

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“…However, traditional PGMs can only be used to measure blood glucose levels. To broaden the application range of glucose meters, we and others have coupled an aptamer, DNAzyme, or antibodies to invertase or amylases, thus converting different nonglucose targets into glucose. − Based on this principle, various sensing strategies using PGM as the readout were reported. − However, because the PGMs are used to detect blood glucose that is normally in the millimolar range, the sensitivities of most PGM-based sensors are still low, especially for small molecules. ,,− To address this issue, we report herein a general aptasensing platform based on a PGM and DNAzyme signal amplification for the detection of small molecules such as mycotoxins, resulting in highly sensitive detection of small molecules by a simple PGM.…”

Section: Introductionmentioning

confidence: 99%

DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter

Zhang

Luan

Xiong

et al. 2021

ACS Appl. Mater. Interfaces

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Aptamer-based sensors have emerged as a major platform for detecting small-molecular targets, because aptamers can be selected to bind these small molecules with higher affinity and selectivity than other receptors such as antibodies. However, portable, accurate, sensitive, and affordable detection of these targets remains a challenge. In this work, we developed an aptasensing platform incorporating magnetic beads and a DNAzyme for signal amplification, resulting in high sensitivity. The biosensing platform was constructed by conjugating a biotin-labeled aptamer probe of small-molecular targets such as toxins and a biotinlabeled substrate strand on magnetic beads, and the DNAzyme strand hybridized with the aptamer probe to block the substrate cleavage activity. The specific binding of the small-molecular target by the aptamer probe can replace the DNAzyme strand and then induce the hybridization between the DNAzyme strand and substrate strand, and the iterative signal amplification reaction of hydrolysis and cleavage of the substrate chain occurs in the presence of a metal ion cofactor. Using invertase to label the substrate strand, the detection of small molecules of the toxin is successfully transformed into the measurement of glucose, and the sensitive analysis of small molecules such as toxins can be realized by using the household portable glucose meter as a readout. This platform is shown to detect ochratoxin, a common toxin in food, with a linear detection range of 5 orders of magnitude, a low detection limit of 0.88 pg/mL, and good selectivity. The platform is easy to operate and can be used as a potential choice for quantitative analysis of small molecules, at home or under point-of-care settings. Moreover, by changing and designing the aptamer probe and the arm of DNAzyme strand, it can be used for the analysis of other analytes.

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“…Sucrose might be converted into glucose using cleaved DNA tagged with invertase, leading to a color change from yellow to green as measured by PGM. The elevated PGM signal correlates with PDGF-BB concentrations ranging from 3.16 × 10 − 16 to 3.16 × 10 − 12 M, with a LOD of 0.11 fM [ 86 ].…”

Section: Dnazymes-assisted Aptasensors For Cancer Detectionmentioning

confidence: 99%

The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors

et al. 2022

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Clinical diagnostics rely heavily on the detection and quantification of cancer biomarkers. The rapid detection of cancer-specific biomarkers is of great importance in the early diagnosis of cancers and plays a crucial role in the subsequent treatments. There are several different detection techniques available today for detecting cancer biomarkers. Because of target-related conformational alterations, high stability, and target variety, aptamers have received considerable interest as a biosensing system component. To date, several sensitivity-enhancement strategies have been used with a broad spectrum of nanomaterials and nanoparticles (NPs) to improve the limit and sensitivity of analyte detection in the construction of innovative aptasensors. The present article aims to outline the research developments on the potential of DNAzymes-based aptasensors for cancer biomarker detection. Graphical Abstract

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Portable aptamer biosensor of platelet-derived growth factor-BB using a personal glucose meter with triply amplified

Cited by 49 publications

References 52 publications

Recent Advances in Aptamer Discovery and Applications

Recent Advances in Aptamer Discovery and Applications

DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter

The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors

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