Portable ATP bioluminescence sensor with high specificity for live Escherichia coli O157:H7 strain synergistically enhanced by orientated phage-modified stir bar extraction and bio-proliferation

Cao, Cong; Wang, Min; Zhang, Dingyi; Yu, Shaoning; Xie, Hongzhen; Wang, Qiqin; Yu, Zhenzhong; Gan, Ning

doi:10.1016/j.bios.2022.114852

Cited by 28 publications

(13 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is no requirement to take a large amount of blood or kill animals, but one only needs to collect the high immunity eggs laid by immune hens to obtain the corresponding specific antibodies. 11,12 Moreover, IgY is a kind of high affinity antibody with persistent titer. Experiments have demonstrated that each egg can produce about 40-500 mg IgY.…”

Section: Introductionmentioning

confidence: 99%

Bioluminescence assay for rapid detection of live Staphylococcus aureus based on the enrichment of egg yolk antibody modified magnetic metal organic framework immunobeads

Zeng,

Zhou,

et al. 2024

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Bioluminescence assay for rapid detection of live Staphylococcus aureus based on the enrichment of egg yolk antibody modified magnetic metal organic framework immunobeads

Zeng,

Zhou,

et al. 2024

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chen et al [ 25 ] prepared a phage-conjugated magnetic probe for the detection of Escherichia coli ( E. coli ) and were able to detect 10 4 CFU/mL of E. coli within 2.5 h with good specificity. Our group [ 26 ] has recently fabricated a portable ATP bioluminescence sensor with high specificity for live Escherichia coli O157:H7 strain, synergistically enhanced by orientated phage-modified stir bar extraction and bio-proliferation. This is suitable for on-site detection of live E. coli O157:H7 with a low detection limit of 30 CFU/mL within 30 min.…”

Section: Introductionmentioning

confidence: 99%

Dual-Mode Biosensor for Simultaneous and Rapid Detection of Live and Whole Salmonella typhimurium Based on Bioluminescence and Fluorescence Detection

Liu

et al. 2023

Biosensors

Self Cite

View full text Add to dashboard Cite

Both live and dead Salmonella typhimurium (S.T) are harmful to human health, but there are differences in pathological mechanism, dosage, and security. It is crucial to develop a rapid and simultaneous assay to distinguish and quantify live and dead S.T in foods. Herein, one dual-mode biosensor for simultaneous detection of live and dead S.T was fabricated based on two phage probes, using portable bioluminescence and fluorescent meter as detectors, respectively. Firstly, a magnetic phage capture probe (M-P1) and a phage signal tag (P2-S) labeled with SYTO 13 fluorescent dye were prepared, respectively. Both M-P1 and P2-S can specifically conjugate with S.T to form a magnetic sandwich complex. After magnetic separation, the isolated complex can emit a fluorescent signal under an excited 365 nm laser, which can reflect the total amount of S.T. Afterwards, the lysozyme was added to decompose the captured live S.T, which can release ATP and produce a bioluminescent signal corresponding to the live S.T amount. The dead S.T concentration can be deduced by the difference between total and live examples. The detection limit of 55 CFU/mL for total S.T and 9 CFU/mL for live ones was within 20 min. The assay was successfully employed in milk samples and prospectively for on-site screening of other dead and live bacteria, while changing the phages for the targets.

show abstract

“…Moreover, the low ATP content (∼10 –18 mol per cell) and bacteriolytic efficiency (generally less than 50%) limit the sensitivity of the traditional BL method (∼10 3 CFU/mL). − Our previous work introduced the phage proliferation process to realize strain-level specificity and increase ATP content. It achieved highly sensitive detection for Escherichia coli O157:H7 with a limit of detection (LOD) of 30 CFU/mL . However, the time-consuming proliferation (30 min) and sterilization of the proliferation medium make it unsuitable for fieldwork.…”

mentioning

confidence: 99%

Versatile Platinum Nanoparticles-Decorated Phage Nanozyme Integrating Recognition, Bacteriolysis, and Catalysis Capabilities for On-Site Detection of Foodborne Pathogenic Strains Vitality Based on Bioluminescence/Pressure Dual-Mode Bioassay

You,

Ma,

et al. 2024

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

Sensitive and on-site discrimination of live and dead foodborne pathogenic strains remains a significant challenge due to the lack of appropriate assay and signal probes. In this work, a versatile platinum nanoparticle-decorated phage nanozyme (P2@PtNPs) that integrated recognition, bacteriolysis, and catalysis was designed to establish the bioluminescence/pressure dual-mode bioassay for on-site determination of the vitality of foodborne pathogenic strains. Benefiting from the bacterial strain-level specificity of phage, the target Salmonella typhimurium (S.T) was specially captured to form sandwich complexes with P2@PtNPs on another phage-modified glass microbead (GM@P1). As the other part of the P2@PtNPs nanozyme, the introduced PtNPs could not only catalyze the decomposition of hydrogen peroxide to generate a significant oxygen pressure signal but also produce hydroxyl radicals around the target bacteria to enhance the bacteriolysis of phage and adenosine triphosphate release. It significantly improved the bioluminescence signal. The two signals corresponded to the total and live target bacteria counts, so the dead target could be easily calculated from the difference between the total and live target bacteria counts. Meanwhile, the vitality of S.T was realized according to the ratio of live and total S.T. Under optimal conditions, the application range of this proposed bioassay for bacterial vitality was 10 2 −10 7 CFU/mL, with a limit of detections for total and live S.T of 30 CFU/mL and 40 CFU/mL, respectively. This work provides an innovative and versatile nanozyme signal probe for the on-site determination of bacterial vitality for food safety.

show abstract

Portable ATP bioluminescence sensor with high specificity for live Escherichia coli O157:H7 strain synergistically enhanced by orientated phage-modified stir bar extraction and bio-proliferation

Cited by 28 publications

References 32 publications

Bioluminescence assay for rapid detection of live Staphylococcus aureus based on the enrichment of egg yolk antibody modified magnetic metal organic framework immunobeads

Bioluminescence assay for rapid detection of live Staphylococcus aureus based on the enrichment of egg yolk antibody modified magnetic metal organic framework immunobeads

Dual-Mode Biosensor for Simultaneous and Rapid Detection of Live and Whole Salmonella typhimurium Based on Bioluminescence and Fluorescence Detection

Versatile Platinum Nanoparticles-Decorated Phage Nanozyme Integrating Recognition, Bacteriolysis, and Catalysis Capabilities for On-Site Detection of Foodborne Pathogenic Strains Vitality Based on Bioluminescence/Pressure Dual-Mode Bioassay

Contact Info

Product

Resources

About