Portable, low cost and sensitive cavity enhanced absorption (CEA) detection

Teggert, Andrew; Datta, Harish K.; McIntosh, Steven; Warden, Barry; Bateson, Simon; Abugchem, Fathi; Ali, Zulfiqur

doi:10.1039/d0an01852j

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…The particles (3D dots) are around 50 to 100 µm in diameter. We have previously described use of photopolymer inkjet 3D printing (i3DP) for creation of a microbioreactor with different functional layers joined by structured adhesive film [145] as well as fast and low cost prototyping of the mechanical and structural elements for a highly sensitive optical cavity enhanced absorption (CEA) detection system [146] (Figure 5). Vat polymerisation-comprising stereolithography (SLA) and digital light processing (DLP)-are amongst the most widely used 3D printing approaches and use UV to cure resin and build a 3D printed object, essentially involving photochemical synthesis of materials.…”

Section: D Printingmentioning

confidence: 99%

Fabrication Methods for Microfluidic Devices: An Overview

2021

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Microfluidic devices offer the potential to automate a wide variety of chemical and biological operations that are applicable for diagnostic and therapeutic operations with higher efficiency as well as higher repeatability and reproducibility. Polymer based microfluidic devices offer particular advantages including those of cost and biocompatibility. Here, we describe direct and replication approaches for manufacturing of polymer microfluidic devices. Replications approaches require fabrication of mould or master and we describe different methods of mould manufacture, including mechanical (micro-cutting; ultrasonic machining), energy-assisted methods (electrodischarge machining, micro-electrochemical machining, laser ablation, electron beam machining, focused ion beam (FIB) machining), traditional micro-electromechanical systems (MEMS) processes, as well as mould fabrication approaches for curved surfaces. The approaches for microfluidic device fabrications are described in terms of low volume production (casting, lamination, laser ablation, 3D printing) and high-volume production (hot embossing, injection moulding, and film or sheet operations).

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Section: D Printingmentioning

confidence: 99%

Fabrication Methods for Microfluidic Devices: An Overview

2021

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“…Another emerging vista in the design of sensing platforms for clinical biomarkers is the fabrication of portable devices using 3D printing. A recent approach had employed 3D printing to design the mechanical components of a portable sensor platform that used optical cavity absorption for sensitive quantification of PCT, CRP and IL-6 [55]. This design strategy could well be explored for fabricating electrochemical sensing chips in the near future.…”

Section: Emerging Directionsmentioning

confidence: 99%

Electrochemical Immunosensors for Quantification of Procalcitonin: Progress and Prospects

et al. 2021

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Human procalcitonin (PCT) is a peptide precursor of the calcium-regulating hormone calcitonin. Traditionally, PCT has been used as a biomarker for severe bacterial infections and sepsis. It has also been recently identified as a potential marker for COVID-19. Normally, serum PCT is intracellularly cleaved to calcitonin, which lowers the levels of PCT (<0.01 ng/mL). In severe infectious diseases and sepsis, serum PCT levels increase above 100 ng/mL in response to pro-inflammatory stimulation. Development of sensors for specific quantification of PCT has resulted in considerable improvement in the sensitivity, linear range and rapid response. Among the various sensing strategies, electrochemical platforms have been extensively investigated owing to their cost-effectiveness, ease of fabrication and portability. Sandwich-type electrochemical immunoassays based on the specific antigen–antibody interactions with an electrochemical transducer and use of nanointerfaces has augmented the electrochemical response of the sensors towards PCT. Identification of a superior combination of electrode material and nanointerface, and translation of the sensing platform into flexible and disposable substrates are under active investigation towards development of a point-of-care device for PCT detection. This review provides an overview of the existing detection strategies and limitations of PCT electrochemical immunosensors, and the emerging directions to address these lacunae.

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“…In continuous microfluidics, several methods have been explored to enhance the detection sensitivity, including specific channel geometries [e.g., U/Z-shape channel ( Ottevaere et al, 2015 ; Yang et al, 2017 )], multi-pass cell ( Llobera et al, 2007 ; Owens and Argueta-Diaz, 2022 ) and cavity-enhanced optics ( Billot et al, 2008 ; Rushworth et al, 2015 ; Teggert et al, 2021 ). Some of those approaches were also demonstrated in droplet microfluidics.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive absorbance measurement using droplet microfluidics integrated with an oil extraction and long pathlength detection flow cell

Lu,

Lunn,

Nightingale

et al. 2024

Front. Chem.

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In droplet microfluidics, UV-Vis absorption spectroscopy along with colorimetric assays have been widely used for chemical and biochemical analysis. However, the sensitivity of the measurement can be limited by the short optical pathlength. Here we report a novel design to enhance the sensitivity by removing oil and converting the droplets into a single-phase aqueous flow, which can be measured within a U-shape channel with long optical pathlength. The flow cells were fabricated via 3D printing. The calibration results have demonstrated complete oil removal and effective optical pathlengths similar to the designed channel lengths (from 5 to 20 mm). The flow cell was further employed in a droplet microfluidic-based phosphate sensing system. The measured phosphate levels displayed excellent consistency with data obtained from traditional UV spectroscopy analysis. This flow cell design overcomes the limitations of short optical pathlengths in droplet microfluidics and has the potential to be used for in situ and continuous monitoring.

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Portable, low cost and sensitive cavity enhanced absorption (CEA) detection

Abstract: Absorption is a widely used technique for a range of different applications. It has lower sensitivity than many other techniques such as fluorescence which has 100 to 1000 times higher...

Cited by 3 publications

References 28 publications

Fabrication Methods for Microfluidic Devices: An Overview

Fabrication Methods for Microfluidic Devices: An Overview

Electrochemical Immunosensors for Quantification of Procalcitonin: Progress and Prospects

Highly sensitive absorbance measurement using droplet microfluidics integrated with an oil extraction and long pathlength detection flow cell

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