Portable molecular diagnostic instruments in microbiology: current status

Lepej, Snježana Židovec; Poljak, Mario

doi:10.1016/j.cmi.2019.09.017

Cited by 15 publications

(6 citation statements)

References 50 publications

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“…As the present work was a proof of principle, a lab PCR was used to verify the feasibility of the system. Portable PCR have already been presented in [14,18,[23][24][25], and the aim of future work is to miniaturize the system for direct PCR detection in order to obtain a transportable system for in-field measurements.…”

Section: Discussionmentioning

confidence: 99%

Sensitive Detection of E. coli in Artificial Seawater by Aptamer-Coated Magnetic Beads and Direct PCR

et al. 2019

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Section: Discussionmentioning

confidence: 99%

Sensitive Detection of E. coli in Artificial Seawater by Aptamer-Coated Magnetic Beads and Direct PCR

et al. 2019

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“…The advantages are numerous: such as a faster turnaround time for the clinician and an excellent clinical performance at affordable prices. Today, molecular testing is increasingly taking the place of conventional culture-based methods [25][26][27].…”

Section: Cleaning and Disinfectionmentioning

confidence: 99%

Nursery outbreak caused by enteroaggregative Escherichia coli serogroup O111:H21

Tré-Hardy

Rauw

Blairon

et al. 2023

APMIS

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This study describes, for the first time, the occurrence of an epidemic of enteroaggregative Escherichia coli (EAEC) O111: H21 in a Belgian nursery and describes a practical approach concerning its management. Few data exist in the literature on this type of outbreak. Clinical and microbiological investigations were needed to find a link between the cases and identify the causative agent. The microbiological procedure followed was first based on conventional analyses: isolation using selective cultures, identification by MALDI-TOF MS, antibiogram, determination of the serogroup by agglutination, then whole genome sequencing. A total of 7/21 children were infected with this pathogen. Four cases could be confirmed by a molecular technique, wgMLST, as belonging to the same bacterial clone. The action plan put in place focused on symptomatic case eviction, strict general hygiene precaution as well as specific cleaning and disinfection measures. The epidemic did last only a few days. It appears important, in the context of an epidemic, that clinical laboratories standardize their practice by equipping themselves with molecular techniques such as a multiplex which does not focus only on the serotype O157:H7 and which make it possible to distinguish the different pathotypes of E. coli by targeting several virulence genes (stx, aggR. . .). However, cost/effectiveness studies are awaited to confirm the interest of a systematic search by molecular method for the pathogen involved in a suspected outbreak occurring in a nursery.

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“…However, endemic regions may qualify for a price discount and the GeneXpert platform is already established in many LMIC for tuberculosis and other pathogens, such as HIV and other sexually-transmitted infections (73)(74)(75)(76). HBV and HCV cartridges are available for viral load (VL) quantification in serum/plasma or by fingerstick (HCV only) and thus are applicable as a near-patient POC test that would be performed in a healthcare facility for patient management purposes.…”

Section: Nucleic-acid-based Rdtmentioning

confidence: 99%

Rapid Diagnostics for Hepatitis B and C Viruses in Low- and Middle-Income Countries

Shenge¹,

Osiowy

2021

Front.Virol.

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The global health challenge posed by hepatitis B virus (HBV) and hepatitis C virus (HCV) persists, especially in low-and-middle-income countries (LMICs), where underdiagnosis of these viral infections remains a barrier to the elimination target of 2030. HBV and HCV infections are responsible for most liver-related mortality worldwide. Infected individuals are often unaware of their condition and as a result, continue to transmit these viruses. Although conventional diagnostic tests exist, in LMIC they are largely inaccessible due to high costs or a lack of trained personnel, resulting in poor linkage to care and increased infections. Timely and accurate diagnosis is needed to achieve elimination of hepatitis B and C by the year 2030 as set out by the World Health Organization Global Health Sector Strategy. In this review rapid diagnostic tests allowing for quick and cost-effective screening and diagnosis of HBV and HCV, are discussed, as are their features, including suitability, reliability, and applicability in LMIC, particularly those within Africa.

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Portable molecular diagnostic instruments in microbiology: current status

Cited by 15 publications

References 50 publications

Sensitive Detection of E. coli in Artificial Seawater by Aptamer-Coated Magnetic Beads and Direct PCR

Sensitive Detection of E. coli in Artificial Seawater by Aptamer-Coated Magnetic Beads and Direct PCR

Nursery outbreak caused by enteroaggregative Escherichia coli serogroup O111:H21

Rapid Diagnostics for Hepatitis B and C Viruses in Low- and Middle-Income Countries

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