Portable Multi-Frequency Impedance-Sensing Device for Bacteria Classification in a Flowing Liquid

Bouzid, Karim; Das, Partha Sarati; Boudreau, Denis; Carrara, Sandro; Gosselin, B

doi:10.1109/newcas52662.2022.9841999

Cited by 2 publications

(6 citation statements)

References 20 publications

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“…From the Randles 52 model and from the impedance model of single-shelled cells 41,42 , it is possible to deduce the optimal excitation frequency range for microorganism characterization, which is found to be between 100 kHz and 10 MHz. For frequencies lower than 100 kHz, the sensibility of the sensor to microparticles is reduced considering that the electrical double-layer (EDL) and ionic diffusion from the Warburg element dominate the measured impedance 38,43,45 . Above 10 MHz, the PCB dielectric begins to shunt the channel impedance, and the parasitics of the measurement electronics significantly reduces the precision of the results.…”

Section: Principles Of Impedance-flow Cytometrymentioning

confidence: 99%

“…The whole fabrication process is described in 38 . A mold is initially drawn on a CAD software such as Solidworks.…”

Section: Microchannelmentioning

confidence: 99%

“…To fill this gap, we present here a low-cost portable impedance biosensor which improves the authors previous sensor design 38 and concepts from printed circuit board integrated directly in a microfluidic device 39 . The presented device can autonomously monitor the impedance of large microorganisms at a high throughput directly in their own natural habitats without using any harmful chemicals, and determines their characteristics based on their impedance profile using EIS and IFC.…”

Section: Introductionmentioning

confidence: 97%

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Portable Impedance-Sensing Device for Microorganism Characterization in the Field

Bouzid

Greener

Carrara

et al. 2023

Preprint

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A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their applicability. This study proposes a portable microfluidic device based on impedance flow-cytometry and electrical impedance spectroscopy that can detect and quantify the size of microparticles larger than 45 μm, such as algae and microplastics. The system is low cost ($300), portable (5 cm × 5 cm), low-power (1.2 W), and easily fabricated utilizing a 3D-printer and industrial printed circuit board technology. The main novelty we demonstrate is the use of square wave excitation signal for impedance measurements with quadrature phase-sensitive detectors. A linked algorithm removes the errors associated to higher order harmonics. After validating the performance of the device for complex impedance models, we used it to detect and differentiate between polyethylene microbeads of sizes between 63 μm and 83 μm, and buccal cells between 45 μm and 70 μm. A precision of 3% is reported for the measured impedance and a minimum size of 45 μm is reported for the particle characterization.

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Section: Principles Of Impedance-flow Cytometrymentioning

confidence: 99%

“…The whole fabrication process is described in 38 . A mold is initially drawn on a CAD software such as Solidworks.…”

Section: Microchannelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Portable Impedance-Sensing Device for Microorganism Characterization in the Field

Bouzid

Greener

Carrara

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…From the Randles 53 model and from the impedance model of single-shelled cells 42,43 , it is possible to deduce the optimal excitation frequency range for microorganism characterization, which is found to be between 100 kHz and 10 MHz. For frequencies lower than 100 kHz, the sensibility of the sensor to microparticles is reduced considering that the electrical double-layer (EDL) and ionic diffusion from the Warburg element dominate the measured impedance 38,44,46 . Above 10 MHz, the PCB dielectric begins to shunt the channel impedance, and the parasitics of the measurement electronics significantly reduces the precision of the results.…”

Section: Principles Of Impedance-flow Cytometrymentioning

confidence: 99%

“…To fill this gap, we present here a low-cost portable impedance biosensor which improves the authors previous sensor design 38,39 and concepts from printed circuit board integrated directly in a microfluidic device 40 . The presented device can autonomously monitor the impedance of large microorganisms at a high throughput directly in their own natural habitats without using any harmful chemicals, and determines their characteristics based on their impedance profile using EIS and IFC.…”

mentioning

confidence: 97%

Portable impedance-sensing device for microorganism characterization in the field

Bouzid

Greener

Carrara

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their applicability. This study proposes a portable microfluidic device based on impedance flow-cytometry and electrical impedance spectroscopy that can detect and quantify the size of microparticles larger than 45 µm, such as algae and microplastics. The system is low cost ($300), portable (5 cm $$\times$$ × 5 cm), low-power (1.2 W), and easily fabricated utilizing a 3D-printer and industrial printed circuit board technology. The main novelty we demonstrate is the use of square wave excitation signal for impedance measurements with quadrature phase-sensitive detectors. A linked algorithm removes the errors associated to higher order harmonics. After validating the performance of the device for complex impedance models, we used it to detect and differentiate between polyethylene microbeads of sizes between 63 and 83 µm, and buccal cells between 45 and 70 µm. A precision of 3% is reported for the measured impedance and a minimum size of 45 µm is reported for the particle characterization.

show abstract

Portable Multi-Frequency Impedance-Sensing Device for Bacteria Classification in a Flowing Liquid

Cited by 2 publications

References 20 publications

Portable Impedance-Sensing Device for Microorganism Characterization in the Field

Portable Impedance-Sensing Device for Microorganism Characterization in the Field

Portable impedance-sensing device for microorganism characterization in the field

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