Portable optical immunosensor for highly sensitive detection of microcystin-LR in water samples

Detection of microcystins in environmental samples using surface plasmon resonance biosensor

“…Furthermore, a comparison of the other biosensors for MCs detection is summarized in Table 1, in contrast to the other techniques such as the electrochemical and evanescent wave allfiber biosensors for MCs detection [7,9,[11][12][13], the present SPR immunosensor was not as highly sensitive as the biosensors above, which may be due to the different antibodies, assay strategies, and technique platforms. However, there is still further scope of the SPR immunoassay sensitivity improvement since antibodyamplification strategies have been successfully applied in other biochemical molecules analyses [21,22].…”

“…However, these methods require either complex operation or expensive materials and devices. To circumvent these drawbacks, several research groups have made the attempts to develop biosensors for MCs detection, such as molecularly imprinted polymer (MIP)-based sensor [5], electrochemical immunosensors [6][7][8][9], and evanescent wave all-fiber immunosensors [10,11]. There have been the reports that described the application of test strips for rapid MCs analysis in situ [12,13]; however, it is still challenging to develop a simple, rapid, and sensitive method for MCs analysis.…”

Trends and Perspectives in Immunosensors

“…However, if there is antigen present, the detection Ab fluoresces due to its excitation by the evanescent wave. Long et al [98] developed a fluorescent fibre-optic immunosensor for the detection of microcystin-LR in water samples. The inhibition assay was carried out with the level of Abs binding to microcystin-LR-ovalbumin conjugate covalently immobilised to the fibre optic surface depending on the concentration of microcystin-LR in the water samples.…”

Emerging (Bio)Sensing Technology for Assessing and Monitoring Freshwater Contamination - Methods and Applications

“…They are presented in table 2 (Almeida et al, 2006;Allum et al, 2008;Dawan et al, 2011;Ding & Mutharasan, 2010;Gregora & Marsálek, 2005;Hu et al, 2008;Lindner et al, 2009;Long et al, 2009Long et al, , 2010Loypraseta et al, 2008;Pyo et al, 2005Pyo et al, , 2006Pyo & Jin, 2007;Queirós et al, 2011;Sheng et al, 2007;Xia et al, 2010Xia et al, , 2011Zhang et al, 2007). Optical immuno-based techniques are by large the most widely techniques that have been developed for the detection of MCs Hu et al, 2008;Long et al, 2010;Loypraseta et al, 2008;Pyo et al, 2005Pyo et al, , 2006Pyo & Jin, 2007;Sheng et al, 2007;Xia et al, 2010).…”

“…They also show potential advantages such as miniaturization, sensitivity, costeffectiveness, and capability of real-time rapid measurements (Long et al, 2009). A portable trace organic pollutant analyzer based on the principle of immunoassay and total internal reflection fluorescence was developed by Long et al.…”