Portable reduced graphene oxide biosensor for detection of rabies virus in bats using nasopharyngeal swab samples

Challhua, Ronaldo; Akashi, L.; Zuñiga, Jose; Batista, Helena Beatriz de Carvalho Ruthner; Moratelli, Ricardo; Champi, A.

doi:10.1016/j.bios.2023.115291

Cited by 15 publications

(4 citation statements)

References 43 publications

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“…W ater-borne viruses pose a serious threat to public health and safety. 1,2 Among them, pathogens such as Norovirus and Rotavirus are well-known causative factors that can lead to nonbacterial gastroenteritis in humans. 3,4 The World Health Organization has estimated that millions of people worldwide die every year due to infections caused by Norovirus or Rotavirus.…”

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confidence: 99%

“…12,13 In recent years, a series of novel PCR-free virus nucleic acid detection methods have been developed, such as surfaceenhanced Raman spectroscopy, 14,15 mass spectrometry, 16,17 fluorescence spectroscopy, 5,18 field-effect transistors, 19 and other techniques. 1,20 All of these technologies have greatly promoted the development of nucleic acid detection technologies. However, few of these methods have been reported to be applied to the detection of viruses exposed to real environments.…”

mentioning

confidence: 99%

“…Water-borne viruses pose a serious threat to public health and safety. , Among them, pathogens such as Norovirus and Rotavirus are well-known causative factors that can lead to nonbacterial gastroenteritis in humans. , The World Health Organization has estimated that millions of people worldwide die every year due to infections caused by Norovirus or Rotavirus. , However, due to the low recognition sensitivity of biological antibodies, traditional immune antigen analysis methods were difficult to meet the detection requirements for multiple types and low loads of exposed viruses in the aqueous environment. , Similarly, polymerase chain reaction (PCR)-based nucleic acid detection methods were equally difficult to satisfy the demand for high-throughput detection of multiplex exposed virus due to their low detection throughput. , Although multiplex PCR techniques have been developed for the simultaneous detection of multiplex nucleic acid sequences, the competition and interference between primer pairs make them highly susceptible to cross-interference when dealing with complex environmental media. , In addition, the high technical requirements of PCR methods, and the complex and time-consuming amplification process make it difficult to meet the demand for immediate detection in the field of environmental analysis . Therefore, there are severe barriers to the application of traditional antigen analysis and nucleic acid detection methods for the detection of viruses exposed to the aqueous environment.…”

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confidence: 99%

“…As nucleic acid detection remains the “gold standard” for virus detection, the development of new methods for PCR-free virus nucleic acid detection has been identified as the key priorities in addressing “major global health challenges”. , In recent years, a series of novel PCR-free virus nucleic acid detection methods have been developed, such as surface-enhanced Raman spectroscopy, , mass spectrometry, , fluorescence spectroscopy, , field-effect transistors, and other techniques. , All of these technologies have greatly promoted the development of nucleic acid detection technologies. However, few of these methods have been reported to be applied to the detection of viruses exposed to real environments.…”

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confidence: 99%

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Distance-Regulated Photoelectrochemical Sensor “Signal-On” and “Signal-Off” Transitions for the Multiplexed Detection of Viruses Exposed in the Aquatic Environment

Li,

Wang

et al. 2023

Anal. Chem.

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Photochemical (PEC) sensors were severely limited for multiplex detection applications due to the cross interference between multiplex signals at the single recognition interface. In this work, a distance-regulated PEC sensor was developed for multiplex detection by using an i-Motif sequence with conformational transformation activity as the signal transduction unit. Through dynamic regulation of the spatial distance between the end site of the functional sequence and the electrode material, the photogenerated electrons on the surface of the sensor were directionally transferred. Thus, a PEC sensor with "signal-on" and "signal-off" dual signal output modes was developed for simultaneous detection of multitarget molecules. Combining isothermal nucleic acid amplification, the PEC sensor constructed in this work was successfully applied to the detection of two virus (Norovirus and Rotavirus) nucleic acid sequences. Under the optimal condition, this bioassay protocol exhibits a linear range of 0.01−100 nM for both viruses with detection limits of 0.72 and 0.53 pM, respectively. In this study, a stimulus-mediated distance regulation strategy successfully addressed the transduction of multiplex detection signals at the single recognition interface of the PEC sensor. It is expected that the technical barriers to multiplex detection of PEC sensors will be overcome and the application of PEC sensing technology will be expanded in the field of environmental analysis.

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confidence: 99%