Portable rotary PCR system for real-time detection of <i>Pseudomonas aeruginosa</i> in milk

Song, Weidu; Zhang, Chuanhao; Lin, Huichao; Zhang, Taiyi; Liu, Haixia; Huang, Xiaowen

doi:10.1039/d3lc00401e

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…The device, as depicted in Figure 5 A, can carry out the thermal cycling process required for PCR by rotating the chamber containing the sample. Sequentially placing the reaction tube on heating zones with different temperatures rather than changing or cycling a single heat block’s temperature is popular in the field of microfluidics and POCT research [ 57 , 58 , 59 , 60 ]. It effectively reduces the time required to finish PCR by eliminating the time required for ramping up and down the heat block.…”

Section: Resultsmentioning

confidence: 99%

“…The device presented here is designed with a focus on the on-site diagnostic application of customized heater fabricating technology, resulting in a relatively longer detection time compared to other PCR devices for POCT. However, previous research [ 58 ] has demonstrated that optimization of temperature conditions to enable annealing and extension simultaneously can shorten detection time, as opposed to heaters with three temperature zones [ 59 ]. By applying this approach to the device presented in this study and fabricating a heater with two temperature zones required for denaturation and annealing/extension, we can expect a reduction in detection time.…”

Section: Resultsmentioning

confidence: 99%

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Customizable Nichrome Wire Heaters for Molecular Diagnostic Applications

Lim,

Han,

Thang

et al. 2024

Biosensors

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Accurate sample heating is vital for nucleic acid extraction and amplification, requiring a sophisticated thermal cycling process in nucleic acid detection. Traditional molecular detection systems with heating capability are bulky, expensive, and primarily designed for lab settings. Consequently, their use is limited where lab systems are unavailable. This study introduces a technique for performing the heating process required in molecular diagnostics applicable for point-of-care testing (POCT), by presenting a method for crafting customized heaters using freely patterned nichrome (NiCr) wire. This technique, fabricating heaters by arranging protrusions on a carbon black-polydimethylsiloxane (PDMS) cast and patterning NiCr wire, utilizes cost-effective materials and is not constrained by shape, thereby enabling customized fabrication in both two-dimensional (2D) and three-dimensional (3D). To illustrate its versatility and practicality, a 2D heater with three temperature zones was developed for a portable device capable of automatic thermocycling for polymerase chain reaction (PCR) to detect Escherichia coli (E. coli) O157:H7 pathogen DNA. Furthermore, the detection of the same pathogen was demonstrated using a customized 3D heater surrounding a microtube for loop-mediated isothermal amplification (LAMP). Successful DNA amplification using the proposed heater suggests that the heating technique introduced in this study can be effectively applied to POCT.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Customizable Nichrome Wire Heaters for Molecular Diagnostic Applications

Lim,

Han,

Thang

et al. 2024

Biosensors

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Colorimetric polymerase chain reaction mediated by pH indicator: Rapid detection of drug-resistant nosocomial bacteria

Trinh,

Lee

2025

Talanta

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A reliable, practical and cost-effective portable wireless potentiostat for mobile chemical sensing and biosensing

Wang,

Pan,

Wang

et al. 2024

Meas. Sci. Technol.

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Traditional electrochemical workstations are costly, complex, bulky, and primarily used in laboratories. This study develops a reliable, practical, and cost-effective portable wireless potentiostat to achieve real-time detection on-site and overcome the limitations of traditional electrochemical workstations. The system employs a general-purpose microcontroller unit (MCU), a dual-mode Bluetooth module and cost-effective multi-analog-to-digital converter (multi-ADC) to achieve differential sampling of the LMP91000. The system is equipped with buttons and OLEDs, enabling connection to mobile phones and computers for in-depth data analysis or independent operation. The system was successfully tested with [Fe(CN)6]3-, C6H12O6, and C3H6O3 solutions at concentrations ranging from 0 to 20 mM. The goodness-of-fit (R²) values are 0.984, 0.996, and 0.998, respectively. The average relative standard deviation of the three blank solutions is approximately 3.22%. The detection limits measured (0.003, 0.009, and 0.005 mM) are all lower than the minimum detection concentration (0.2, 0.1, and 0.1 mM). The coefficient of variation for repetitive experiments is less than 5.53%. The device accurately executed chronoamperometry (applied voltage range is ± 1.2 V, current range is ± 882 μA, accuracy is ± 1 %) with high sensitivity and good repeatability. Based on this circuit, a lactic acid detector and a urine glucose detector were developed, which work stably and support long-term operation, proving the stability and reliability of the circuit. Compared to commercial electrochemical workstations, PWES offers remarkable advantages in cost (< $6.4), size (41.5 mm × 76.5 mm), and practicality, making it suitable for a range of applications, including biomedical analysis, food safety, environmental monitoring, and smart wearables.

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Portable rotary PCR system for real-time detection of Pseudomonas aeruginosa in milk

Abstract: A PCR solution is heated by hot air between two heating blocks.

Cited by 4 publications

References 31 publications

Customizable Nichrome Wire Heaters for Molecular Diagnostic Applications

Customizable Nichrome Wire Heaters for Molecular Diagnostic Applications

Colorimetric polymerase chain reaction mediated by pH indicator: Rapid detection of drug-resistant nosocomial bacteria

A reliable, practical and cost-effective portable wireless potentiostat for mobile chemical sensing and biosensing

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