At
present, the use of alternative systems to replenish the lost
functions of hepatic metabolism and partial replacement of liver organ
failure is relevant, due to an increase in the incidence of various
liver disorders, insufficiency, and cost of organs for transplantation,
as well as the high cost of using the artificial liver systems. The
development of low-cost intracorporeal systems for maintaining hepatic
metabolism using tissue engineering, as a bridge before liver transplantation
or completely replacing liver function, deserves special attention.
In vivo applications of intracorporeal fibrous nickel–titanium
scaffolds (FNTSs) with cultured hepatocytes are described. Hepatocytes
cultured in FNTSs are superior to their injections in terms of liver
function, survival time, and recovery in a CCl4-induced
cirrhosis rats’ model. 232 animals were divided into 5 groups:
control, CCl4-induced cirrhosis, CCl4-induced
cirrhosis followed by implantation of cell-free FNTSs (sham surgery),
CCl4-induced cirrhosis followed by infusion of hepatocytes
(2 mL, 107 cells/mL), and CCl4-induced cirrhosis
followed by FNTS implantation with hepatocytes. Restoration of hepatocyte
function in the FNTS implantation with the hepatocytes group was accompanied
by a significant decrease in the level of aspartate aminotransferase
(AsAT) in blood serum compared to the cirrhosis group. A significant
decrease in the level of AsAT was noted after 15 days in the infused
hepatocytes group. However, on the 30th day, the AsAT level increased
and was close to the cirrhosis group due to the short-term effect
after the introduction of hepatocytes without a scaffold. The changes
in alanine aminotransferase (AlAT), alkaline phosphatase (AlP), total
and direct bilirubin, serum protein, triacylglycerol, lactate, albumin,
and lipoproteins were similar to those in AsAT. The survival time
of animals was significantly longer in the FNTS implantation with
hepatocytes group. The obtained results showed the scaffolds’
ability to support hepatocellular metabolism. The development of hepatocytes
in FNTS was studied in vivo using 12 animals using scanning electron
microscopy. Hepatocytes demonstrated good adhesion to the scaffold
wireframe and survival in allogeneic conditions. Mature tissue, including
cellular and fibrous, filled the scaffold space by 98% in 28 days.
The study shows the extent to which an implantable “auxiliary
liver” compensates for the lack of liver function without replacement
in rats.