Pos-437 Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury

Feng, Daniel; Ngov, Cindy; Henley, Nathalie; Boufaied, Nadia; Gerarduzzi, Casimiro

doi:10.1016/j.ekir.2021.03.461

Cited by 1 publication

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“…To investigate the expression of ERPS1 in kidney brosis, 8-week-old male C57BL/6 mice (n = 7) were treated with a single dose of 250 mg/kg of FA (dissolved in 0.3 M sodium bicarbonate) by intraperitoneal injection 25 . The FA-induced kidney brosis mouse model (FA) showed characteristic injured tubules, interstitial brosis, and ECM accumulation [26][27][28] .…”

Section: Methodsmentioning

confidence: 99%

EPRS1-mediated fibroblast activation and mitochondrial dysfunction promote kidney fibrosis

Lee,

Son,

Jeong

et al. 2024

Preprint

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Kidney fibrosis is characterized by an aberrant extracellular matrix accumulation. Glutamyl-prolyl-tRNA synthetase 1 (EPRS1) is a crucial enzyme involved in the synthesis of proline-rich proteins. However, its role in kidney fibrosis remains unclear. The present study found that EPRS1 expression levels were increased in the fibrotic kidneys of patients and mice. We also found that fibroblasts and proximal tubular epithelial cells were major sources of EPRS1 based on single-cell analysis and immunostaining of fibrotic kidneys. C57BL/6 EPRS1tm1b heterozygous knockout (Eprs1+/−) attenuated kidney fibrosis and dysfunction by preventing fibroblast activation and proximal tubular injury. In vitro assays demonstrated that EPRS1-induced Smad3 phosphorylation promotes fibroblast activation, proliferation, and resultant collagen production under transforming growth factor-β treated conditions. We also found that EPRS1 could lead to STAT3 phosphorylation to induce mitochondrial dysfunction in injured proximal tubules. Moreover, first-in-class EPRS1 inhibitor DWN12088 effectively protected against kidney fibrosis and dysfunction. Our findings suggest that EPRS1-mediated extracellular matrix induces kidney fibrosis via fibroblast activation and mitochondrial dysfunction.

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Section: Methodsmentioning

confidence: 99%