Posaconazole treatment of refractory coccidioidomycosis in dogs

Shubitz, Lisa F.; Schlacks, Sallianne; Vishkautsan, Polina; Butkiewicz, Christine D.; Worthing, Kate A.

doi:10.1111/jvim.16282

Cited by 5 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Investigation into the immunopathogenesis of this disease could provide insight into the wide spectrum of clinical manifestations in dogs with coccidioidomycosis [ 4 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Guided by what has been learned from human clinical and mouse experimental studies, the primary objective of the study reported here was to compare constitutive and Coccidioides spp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Host Constitutive and Ex Vivo Coccidioidal Antigen-Stimulated Immune Response in Dogs with Naturally Acquired Coccidioidomycosis

Jaffey

Shubitz

Johnson

et al. 2023

JoF

Self Cite

View full text Add to dashboard Cite

The early innate immune response to coccidioidomycosis has proven to be pivotal in directing the adaptive immune response and disease outcome in mice and humans but is unexplored in dogs. The objectives of this study were to evaluate the innate immune profile of dogs with coccidioidomycosis and determine if differences exist based on the extent of infection (i.e., pulmonary or disseminated). A total of 28 dogs with coccidioidomycosis (pulmonary, n = 16; disseminated, n = 12) and 10 seronegative healthy controls were enrolled. Immunologic testing was performed immediately, without ex vivo incubation (i.e., constitutive), and after coccidioidal antigen stimulation of whole blood cultures. Whole blood cultures were incubated with a phosphate-buffered solution (PBS) (negative control) or a coccidioidal antigen (rCTS1 (105–310); 10 µg/mL) for 24 h. A validated canine-specific multiplex bead-based assay was used to measure 12 cytokines in plasma and cell culture supernatant. Serum C-reactive protein (CRP) was measured with an ELISA assay. Leukocyte expression of toll-like receptors (TLRs)2 and TLR4 was measured using flow cytometry. Dogs with coccidioidomycosis had higher constitutive plasma keratinocyte chemotactic (KC)-like concentrations (p = 0.02) and serum CRP concentrations compared to controls (p < 0.001). Moreover, dogs with pulmonary coccidioidomycosis had higher serum CRP concentrations than those with dissemination (p = 0.001). Peripheral blood leukocytes from dogs with coccidioidomycosis produced higher concentrations of tumor necrosis factor (TNF)-α (p = 0.0003), interleukin (IL)-6 (p = 0.04), interferon (IFN)-γ (p = 0.03), monocyte chemoattractant protein (MCP)-1 (p = 0.02), IL-10 (p = 0.02), and lower IL-8 (p = 0.003) in supernatants following coccidioidal antigen stimulation when compared to those from control dogs. There was no detectable difference between dogs with pulmonary and disseminated disease. No differences in constitutive or stimulated leukocyte TLR2 and TLR4 expression were found. These results provide information about the constitutive and coccidioidal antigen-specific stimulated immune profile in dogs with naturally acquired coccidioidomycosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Host Constitutive and Ex Vivo Coccidioidal Antigen-Stimulated Immune Response in Dogs with Naturally Acquired Coccidioidomycosis

Jaffey

Shubitz

Johnson

et al. 2023

JoF

Self Cite

View full text Add to dashboard Cite

show abstract

Coccidioidomycosis and Paracoccidioidomycosis

Vishkautsan¹,

Sykes²

2021

Greene's Infectious Diseases of the Dog and Cat

View full text Add to dashboard Cite

The dog as a sentinel and animal model for coccidioidomycosis

Ferguson,

Thompson,

Bruyette

et al. 2023

Medical Mycology

View full text Add to dashboard Cite

Coccidioidomycosis is a potentially fatal fungal disease of humans and animals that follows inhalation of Coccidioides spp. arthroconidia in the environment. The disease in dogs resembles that in people, and because dogs may be at increased risk of exposure due to their proximity to the ground and digging behavior, they are valuable models for the disease in humans. Dogs have been sentinels for identification of new regions of endemicity in Washington and Texas. Canine serosurveillance has also been used to predict variables associated with environmental presence of Coccidioides spp. Expansion of the endemic region of coccidioidomycosis with climate change—along with predicted population increases and increased development in the southwest United States—may result in 45.4 million additional people at risk of infection by 2090. Here we provide an overview of the value of dogs as sentinels for the disease and encourage the routine reporting of coccidioidomycosis cases in dogs to public health agencies. We also highlight the value of dogs as naturally-occurring models for studying novel treatment options and preventatives, such as a novel live avirulent coccidioidomycosis vaccine.

show abstract

Posaconazole treatment of refractory coccidioidomycosis in dogs

Cited by 5 publications

References 31 publications

Evaluation of Host Constitutive and Ex Vivo Coccidioidal Antigen-Stimulated Immune Response in Dogs with Naturally Acquired Coccidioidomycosis

Evaluation of Host Constitutive and Ex Vivo Coccidioidal Antigen-Stimulated Immune Response in Dogs with Naturally Acquired Coccidioidomycosis

Coccidioidomycosis and Paracoccidioidomycosis

The dog as a sentinel and animal model for coccidioidomycosis

Contact Info

Product

Resources

About