Position Effects Influencing Intrachromosomal Repair of a Double-Strand Break in Budding Yeast

Abstract

8Repair of a double-strand break (DSB) by an ectopic homologous donor sequence is 1 9 subject to the three-dimensional arrangement of chromosomes in the nucleus of haploid 2 0 budding yeast. The data for interchromosomal recombination suggest that searching 2 1 for homology is accomplished by a random collision process, strongly influenced by the 2 2 contact probability of the donor and recipient sequences. Here we explore how constraints imposed on repair. Specifically, we examined how intrachromosomal repair (Chr2), with a site-specific DSB created on the right arm (position 625kb). Repair . CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under aThe copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/114850 doi: bioRxiv preprint first posted online Mar. 7, 2017;