Positional dependence of particles and cells in microfluidic electrical impedance flow cytometry: origin, challenges and opportunities

Daguerre, Hugo; Solsona, Miguel; Cottet, Jonathan; Gauthier, Michaël; Renaud, Philippe; Bolopion, Aude

doi:10.1039/d0lc00616e

Cited by 86 publications

(50 citation statements)

References 144 publications

(196 reference statements)

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“…Since Schwan, many developments and applications have been made based on bioimpedance analysis. Ayliffe et al made the first microfluidic device with integrated microelectrodes for single-cell impedance measurements [66][67][68][69][70][71]. It consisted of a microchannel (4 × 10 ×150 µm) made of epoxy photoresist on glass and two gold electrodes (8 × 4 µm, with a gap of 7 µm).…”

Section: Microfluidic Impedance Flow Cytometry (Mifc)mentioning

confidence: 99%

“…To achieve this, one of the most used techniques is 3D hydrodynamic focus, which consists of aligning the cells by pushing them with additional fluids in the x, y (2D), and Z (3D) directions. Greater detail on this topic can be found in the work of Daguerre et al [71]. As such, complex impedance is an exciting and valuable electrical biomarker.…”

Section: Microfluidic Impedance Flow Cytometry (Mifc)mentioning

confidence: 99%

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Systematic Review: Microfluidics and Plasmodium

et al. 2021

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Malaria affects 228 million people worldwide each year, causing severe disease and worsening the conditions of already vulnerable populations. In this review, we explore how malaria has been detected in the past and how it can be detected in the future. Our primary focus is on finding new directions for low-cost diagnostic methods that unspecialized personnel can apply in situ. Through this review, we show that microfluidic devices can help pre-concentrate samples of blood infected with malaria to facilitate the diagnosis. Importantly, these devices can be made cheaply and be readily deployed in remote locations.

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Section: Microfluidic Impedance Flow Cytometry (Mifc)mentioning

confidence: 99%

Section: Microfluidic Impedance Flow Cytometry (Mifc)mentioning

confidence: 99%

Systematic Review: Microfluidics and Plasmodium

et al. 2021

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“…Conversely, the other geometry is relatively simple because it can be fabricated on the same plane. In addition, the planar type exhibits higher sensing synergies with a microfluidic channel [ 43 ]. Owing to its non-uniform electric field, the peak signal becomes larger as the particles move closer toward the electrodes.…”

Section: Sensor Design and Fabricationmentioning

confidence: 99%

Microfluidic Airborne Metal Particle Sensor Using Oil Microcirculation for Real-Time and Continuous Monitoring of Metal Particle Emission

et al. 2021

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Airborne metal particles (MPs; particle size > 10 μm) in workplaces result in a loss in production yield if not detected in time. The demand for compact and cost-efficient MP sensors to monitor airborne MP generation is increasing. However, contemporary instruments and laboratory-grade sensors exhibit certain limitations in real-time and on-site monitoring of airborne MPs. This paper presents a microfluidic MP detection chip to address these limitations. By combining the proposed system with microcirculation-based particle-to-liquid collection and a capacitive sensing method, the continuous detection of airborne MPs can be achieved. A few microfabrication processes were realized, resulting in a compact system, which can be easily replaced after contamination with a low-priced microfluidic chip. In our experiments, the frequency-dependent capacitive changes were characterized using MP (aluminum) samples (sizes ranging from 10 μm to 40 μm). Performance evaluation of the proposed system under test-bed conditions indicated that it is capable of real-time and continuous monitoring of airborne MPs (minimum size 10 μm) under an optimal frequency, with superior sensitivity and responsivity. Therefore, the proposed system can be used as an on-site MP sensor for unexpected airborne MP generation in precise manufacturing facilities where metal sources are used.

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“…Planar electrodes have a higher sensitivity, but a complex fabrication process, whereas coplanar electrodes are less sensitive, but simpler and cheaper to fabricate [10,11]. In both configurations, the position of particles is an important parameter for an accurate analysis of these passing particles or cells, as recently discussed by Daguerre et al [12].…”

Section: Introductionmentioning

confidence: 97%

Determining Particle Size and Position in a Coplanar Electrode Setup Using Measured Opacity for Microfluidic Cytometry

et al. 2021

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Microfluidic impedance flow cytometers enable high-throughput, non-invasive, and label-free detection of single-cells. Cytometers with coplanar electrodes are easy and cheap to fabricate, but are sensitive to positional differences of passing particles, owing to the inhomogeneous electric field. We present a novel particle height compensation method, which employs the dependence of measured electrical opacity on particle height. The measured electrical opacity correlates with the particle height as a result of the constant electrical double layer series capacitance of the electrodes. As an alternative to existing compensation methods, we use only two coplanar electrodes and multi-frequency analysis to determine the particle size of a mixture of 5, 6, and 7 µm polystyrene beads with an accuracy (CV) of 5.8%, 4.0%, and 2.9%, respectively. Additionally, we can predict the bead height with an accuracy of 1.5 µm (8% of channel height) using the measured opacity and we demonstrate its application in flow cytometry with yeast. The use of only two electrodes is of special interest for simplified, easy-to-use chips with a minimum amount of instrumentation and of limited size.

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Positional dependence of particles and cells in microfluidic electrical impedance flow cytometry: origin, challenges and opportunities

Abstract: Microfluidic electrical impedance flow cytometry is now a well-known and established method for single-cell analysis. Given the richness of the information provided by impedance measurements, this non-invasive and label-free approach...

Cited by 86 publications

References 144 publications

Systematic Review: Microfluidics and Plasmodium

Systematic Review: Microfluidics and Plasmodium

Microfluidic Airborne Metal Particle Sensor Using Oil Microcirculation for Real-Time and Continuous Monitoring of Metal Particle Emission

Determining Particle Size and Position in a Coplanar Electrode Setup Using Measured Opacity for Microfluidic Cytometry

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