Positional distribution of CLA in TAG of lamb tissues

Paterson, Lyn; Weselake, Randall J.; Mir, P. S.; Mir, Z.

doi:10.1007/s11745-002-0939-6

Cited by 19 publications

(13 citation statements)

References 44 publications

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“…1 and 2 and used for stereospecific analysis to determine if CLA was specifically esterified to a particular position on the glycerol moiety. From the two experiments, there were four muscle samples from steers fed CON, 6% SFO, and 500 IU vitamin E per day, but only two samples from animals fed 3% SFO and 6%SFO with 500 IU vitamin E. The procedure described by Paterson et al (2002) was used for determination of proportion of fatty acids (%) at sn-2 and sn-1/3 position on the TAG. The meat samples were freeze-dried and extracted with ethyl ether to determine total lipid (TL) content.…”

Section: Positional Distribution Of Fatty Acids On Tagmentioning

confidence: 99%

Effect of dietary sunflower oil and vitamin E on Beef cattle performance, carcass characteristics and meat quality

Mir¹,

McAllister²,

Zaman³

et al. 2003

Can. J. Anim. Sci.

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Z. 2003. Effect of dietary sunflower oil and vitamin E on beef cattle performance, carcass characteristics and meat quality. Can. J. Anim. Sci. 83: 53-66. In the present study, we tested the hypothesis that feeding sunflower oil (SFO) containing about 70% linoleic acid would increase conjugated linoleic acids (CLA) content of beef and that the increased CLA levels would improve meat shelf life. Two feeding trials were conducted to determine the effect of SFO supplementation on beef production, carcass characteristics and meat quality parameters. In addition, the visual aspects of beef quality in retail display as influenced by dietary vitamin E were compared with that of the increased CLA derived from dietary SFO. In exp. 1, 72 finishing steers (465 ± 9 kg) were individually fed one of four dietary treatments: an unsupplemented control finishing diet, the control diet supplemented with 6% SFO, 500 IU head -1 d -1 vitamin E or a combination of 6% SFO and 500 IU head -1 d -1 vitamin E for 95 d. Data from in vitro gas production from the diets indicated that oil inclusion reduced rate and lag time prior to initiation of gas production. Dry matter intake (DMI) declined (P < 0.05) due to dietary treatments without affecting average daily gain or feed conversion efficiency. Meat from steers fed all supplements was less red, more discolored and less acceptable after 2 d storage in a display case. In exp. 2, 40 steers (412.5 ± 11 kg), in individual pens, were used in a second completely randomized design experiment to evaluate the effect of supplementation of steam-rolled barley based finishing diets with 0, (CON) 3 or 6% SFO or 500 IU head -1 d -1 vitamin E on performance, carcass, meat characteristics, and fatty acid composition of beef. No difference (P > 0.05) in final liveweight, DMI, average daily weight gain (ADG) and feed efficiency (FE) of steers fed diets was found. Carcass grade fat was higher (P < 0.05) in steers fed the 6% SFO diet than the other diets. Retail acceptance score of meat obtained from steers fed 6% SFO was higher than from steers fed other diets (P < 0.05). In both experiments, the fatty acid composition of the meat indicated that C 16:0 and C 16:1 fatty acid levels were lower (P < 0.05) while C 18:2 was higher (P < 0.05) in longissimus muscle of steers fed 6% SFO than the other diets. CLA content in fat from longissimus muscle was higher in steers fed the 6% SFO than control diets (3.5 vs. 3.0 mg g -1 in exp. 1 and 3.5 vs. 2.0 mg g -1 in exp. 2; P < 0.05). Hence supplementing feedlot diets with SFO for up to 108 d can increase CLA composition of beef. Positional distribution of fatty acids indicated that the majority of the CLA was in the sn-2 position of the triacyglycerol molecule.Key words: Beef cattle, conjugated linoleic acid, fatty acids, meat quality, sunflower oil, vitamin E Mir, P. S., McAllister, T. A., Zaman, S., Morgan Jones, S. D., He, M. L., Aalhus, J. L., Jeremiah, L. E., Goonewardene, L. A., Weselake, R. J. et Mir, Z. 2003. Incidence de l'huile de tournesol et de la vitamine ...

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Section: Positional Distribution Of Fatty Acids On Tagmentioning

confidence: 99%

Effect of dietary sunflower oil and vitamin E on Beef cattle performance, carcass characteristics and meat quality

Mir¹,

McAllister²,

Zaman³

et al. 2003

Can. J. Anim. Sci.

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“…Chardigny et al [9] studied the location of labelled CLA in TAG at either the sn1/3 or sn2 position, and found that the label was recovered in oxidation products when CLA was in the sn1/3 position but was found in the carcass when the labelled CLA was in the sn2 position. These authors found that dairy CLA was uniformly distributed among the three positions of milk fat, as did Paterson et al [10], who found that bioformed CLA from sheep fed safflower oil aggregated to greater proportions at the sn1/3 position in muscle fat. However, Mir et al [11] found that muscle fat from beef steers fed sunflower oil had greater proportions of CLA at the sn2 position, but the amounts were greater when the dietary oil incorporation level was 3% of diet rather than 6%.…”

Section: Introductionmentioning

confidence: 60%

“…Pancreatic lipase (Lipase from porcine pancreas, Type II; Sigma-Aldrich Canada, Oakville, ON) was used to generate sn2-MAG from TAG [10]. Two milligrams of dried TAG wERE suspended in 500 μ L of pancreatic lipase buffer [1 M Tris-HCl, pH 8, containing 10% gum Arabic (wt/v) and 0.23 M CaCl 2 (wt/v)] by sonication.…”

Section: Methodsmentioning

confidence: 99%

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Fatty Acid Composition of Phospholipids and in the Central and External Positions of Triacylglycerol in Muscle and Subcutaneous Fat of Beef Steers Fed Diets Supplemented with Oil Containing n6 and n3 Fatty Acids While Undergoing One of Three 48 h Feed Withdrawal Treatments

et al. 2012

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This study was designed to determine the effects of dietary oil and feed withdrawal treatments on fatty acid composition of phospholipids of triacylglycerol in pars costalis diaphragmatis muscle and subcutaneous fat from the brisket. A 2 × 3 factorial experiment was conducted with crossbred steers with an initial body weight of 280.5 ± 5.8 kg. Steers were fed either a control or an oil containing diet where 5% of the control diet was replaced with an equal mixture sunflower and flax oil while undergoing one of three feed withdrawal treatments: no withdrawal, a single 48 h withdrawal before initiation of fattening at one year of age, or 48 h withdrawal at 8 wk intervals from weaning to initiation of fattening. At time of processing samples of muscle and fat were obtained and analyzed to determine fatty acid composition. Disproportionate distribution of the fatty acids was observed by diet, feed withdrawal regimen and whether the sample was from muscle or fat. Differences are discussed in detail, and our data suggests a special function for the fatty acids that accumulate in specific positions of the triacylglycerol due to treatment.

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“…I). On the contrary, the CLA positional distribution in TAG (sn-2 vs. sn-1/3) of ruminant (lamb) muscle or subcutaneous adipose tissue has been reported to be modified by this dietary factor [17]. Thus, due to its specific rumenic acid distribution within TAG, dairyfat might not be nutritionally equivalent to other ruminant products in terms of rumenic acid availability and activity.…”

Section: Resultsmentioning

confidence: 99%

Complete stereospecific determination of conjugated linoleic acids in triacylglycerol of milk-fat

Valeille

Martin

2004

Reprod. Nutr. Dev.

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-We analyzed two kinds of dairy fat differing in their contents of cis9,trans11-conjugated linoleic acid (cis9,trans11-CLA or rumenic acid), and determined the positional distribution of this CLA-isomer within the three sn-positions of the triacylglycerol. In the high rumenic acid fat (HR), the CLA-isomers amounted to 2.1% of total fatty acids, and 0.8% in the low rumenic acid fat (LR). Over 90% of the total CLA-isomers were in the form of rumenic acid, with an identical isomeric CLA distribution in both fats. The two fats differed mainly with regards to their contents in palmitic acid, α-linolenic acid, and isomers of trans-C18:1. Conversely, our stereospecific determination indicates that the positional distribution of rumenic acid is preserved among both types of fat, and is more specific for the sn-3 position of the triacylglycerol (54 to 64% of the total rumenic acid). Such a positional distribution is believed to be nutritionally relevant.conjugated linoleic acid / milk-fat / positional distribution

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Positional distribution of CLA in TAG of lamb tissues

Cited by 19 publications

References 44 publications

Effect of dietary sunflower oil and vitamin E on Beef cattle performance, carcass characteristics and meat quality

Effect of dietary sunflower oil and vitamin E on Beef cattle performance, carcass characteristics and meat quality

Fatty Acid Composition of Phospholipids and in the Central and External Positions of Triacylglycerol in Muscle and Subcutaneous Fat of Beef Steers Fed Diets Supplemented with Oil Containing n6 and n3 Fatty Acids While Undergoing One of Three 48 h Feed Withdrawal Treatments

Complete stereospecific determination of conjugated linoleic acids in triacylglycerol of milk-fat

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