Positional mapping for amplified DNA sequences on 1q21–q22 in hepatocellular carcinoma indicates candidate genes over-expression

Wong, Nathalie; Chan, Andrew M.; Lee, Siu Wah; Lam, Ellan; To, Ka Fai; Lai, Paul B.S.; Li, Xiao Nan; Liew, C. T.; Johnson, Philip J.

doi:10.1016/s0168-8278(02)00412-9

Cited by 80 publications

(65 citation statements)

References 22 publications

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“…According to previous study, high incidence of C1q copy number gain was found in HCC (60 to 80%) (Nathalie et al, 2001). Many up-regulated cancer-related genes, such as JTB, SHC1, CCT3, and COPA, were observed in HCC located at 1q12-q22 (Wong et al, 2003). COTE1, a novel potential oncogene, identified by our lab, located at chromosome 1q 21.…”

Section: Discussionmentioning

confidence: 69%

Ectopic Overexpression of COTE1 Promotes Cellular Invasion of Hepatocellular Carcinoma

Hai

Huang²,

Tian³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 69%

Ectopic Overexpression of COTE1 Promotes Cellular Invasion of Hepatocellular Carcinoma

Hai

Huang²,

Tian³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…In an attempt to elucidate for the underlying affected genes, positional mapping from our group has previously refined the 1q21-q22 amplicon in hepatocellular carcinoma to three affected loci, in which the overexpression of JTB, SHC1, CCT3 and COPA was indicated. 29 While JTB, SHC1 and COPA may offer the tumorous tissue with proliferative advantages, CCT3 is a molecular chaperone that can enhance cell migration by controlling its downstream targets, namely actin and tubulin. Aside from cell motility, CCT3 overexpression was also considered as an effective predictor between moderately and well-differentiated hepatocellular carcinoma.…”

Section: Discussionmentioning

confidence: 99%

Regional over-representations on chromosomes 1q, 3q and 7q in the progression of hepatitis B virus-related hepatocellular carcinoma

Wong

Lai

et al. 2005

Modern Pathology

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Hepatocellular carcinoma is a highly malignant tumor that is prevalent in Southeast Asia and China, where hepatitis B viral infection is the main etiologic factor. Despite a high incidence of hepatocellular carcinoma developing in patients with viral hepatitis B-induced liver cirrhosis, the molecular events underlying the malignant liver progression remain largely unclear. In an effort to characterize the genetic abnormalities involved in the hepatitis B-related liver carcinogenesis, we performed genome-wide explorations by the technique of comparative genomic hybridization (CGH) on 100 hepatocellular carcinoma tumors that arose from hepatitis B-induced liver cirrhosis. According to the American Joint Committee on Cancer staging, four cases were classified as stage I, 69 as stage II, 23 as stage III and four as stage IV. CGH analysis indicated chromosomal instability in both early (stages I/II) and advanced (stages III/IV) stage tumors, with common gains on 1q, 8q and 17q23-q25, and losses on 4q22-q35, 8p21-p22, 13q14-q21, 16q and 17p identified in both groups (P40.05). Nevertheless, preferential sites of chromosomal defects in relation to hepatocellular carcinoma progression were also identified. Statistical correlations suggested a higher incidence of regional 1q21-q22, 3q22-q28, 7q21-q22 and 7q34-q36 over-representations in association with the advanced stage tumors (Po0.05). In this study, our novel identification of specific chromosomal aberrations in relation to the advanced stage tumors may represent a first step towards mapping genes linked to the progression of hepatocellular carcinoma. Modern Pathology (2005) 18, 686-692, advance online publication, 17 December 2004; doi:10.1038/modpathol.3800345 Keywords: hepatocellular carcinoma; hepatitis B virus; liver cirrhosis; progression Hepatocellular carcinoma is a primary liver malignancy that is highly malignant and rapidly fatal. The dismal clinical outcome is largely due to the majority of hepatocellular carcinoma tumors being asymptomatic during the natural course of the disease, consequently rendering most patients not being diagnosed in time for curative surgery. 1 By the time of clinical presentation, intra-and extrahepatic metastases are also common, which in turn has limited the scope of curative surgery. 2 In addition, the relatively high incidences of hepatocellular carcinoma recurrence, possibly from micrometastasis prior to surgical treatment, has further lowered the 5-year survival rate for individuals diagnosed with hepatocellular carcinoma. 3 The understanding of molecular events involved in the malignant liver progression thus would hold much value in the prognosis for patients with hepatocellular carcinoma.Studies on the molecular pathogenesis have guided the development of gene-based biomarkers in a number of human cancers including breast, colon, prostate and lung. 4 In hepatocellular carcinoma, although genetic characterizations have indicated frequent chromosomal over-representations on 1q, 6p, 8q, 17q and 20q, and deletions on 1p, 4q, ...

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“…Quantitative reverse transcription PCR (qRT-PCR) experiments normalized with the internal reference GAPDH gene were performed using SYBR Green PCR Core reagents kit (Applied Biosystems, Foster City, CA) as previously described. 16 First-strand cDNA was amplified in 25 l reaction with 400 nM primers, 0.25U AmpliTaq Gold DNA polymerase, 1.5 mM MgCl 2 , and dNTPs. No template PCR reactions served as negative controls in all experiments.…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative PCR experiments were performed using SYBR Green PCR Core reagents kit (Applied Biosystems) with the internal reference gene as ␤-globin. 16 Primers used for PFTK1 were (sense) 5Ј-TACAGAATTCCCAGCCGAAA-3Ј (antisense) 5Ј-TCTTC TTCCTGCAGCCTGAT-3Ј and for ␤-globin were (sense) 5Ј-GAAGAGCCAAGGACAGG TAC-3Ј (antisense) 5Ј-CAACTTCATCCACGTTCACC-3Ј, respectively. Threshold cycles were averaged from triplicate reactions and expressed relative to the mean derived from 3 normal human livers.…”

Section: Methodsmentioning

confidence: 99%

Identification of PFTAIRE protein kinase 1, a novel cell division cycle‐2 related gene, in the motile phenotype of hepatocellular carcinoma cells†

Pang

Bai

et al. 2007

Hepatology

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Metastasis is a major cause of cancer morbidity and mortality in individuals with hepatocellular carcinoma (HCC), yet little is known about the underlying molecular basis. Using genetic information derived from chromosome-based comparative genomic hybridization, we have reported previously on regional chromosome 7q21-q22 gains in close association with HCC progression. In this study, we undertook cDNA microarray-based comparative genomic hybridization, to examine the 7q21-q22 region for the involved gene(s) in HCC. High-resolution mapping analysis highlighted 7 candidates, namely PFTAIRE protein kinase 1 (PFTK1), ODAG, CDK6, CAS1, PEX1, SLC25A, and PEG10, within the region. H epatocellular carcinoma (HCC) is the fifth most common cancer worldwide 1 and the third most common cause of cancer-related mortality. One of the reasons for this high mortality is that the tumour usually presents at a stage when curative surgery is no longer feasible because of intrahepatic or extrahepatic metastases. Although the 5-year survival of HCC patients who have undergone hepatectomy has improved in recent years, postoperative intrahepatic recurrence can still be as high as 43% by 2 years after resection, 2 presumably because of preexisting micro-metastases. 3 These observations underscore the urgent need to understand the molecular characteristics and related biological mechanisms in tumor cell dissemination.Despite extensive studies, the underlying genomic alterations in HCC remain poorly understood, 4-6 and few tumor suppressors or proto-oncogenes have been related to metastasis and recurrences. Utilizing genetic information derived from a cohort of 100 HCC tumors analyzed by chromosome-based comparative genomic hybridization (CGH), our group has previously shown several regional chromosome gains, especially over-representations

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Positional mapping for amplified DNA sequences on 1q21–q22 in hepatocellular carcinoma indicates candidate genes over-expression

Cited by 80 publications

References 22 publications

Ectopic Overexpression of COTE1 Promotes Cellular Invasion of Hepatocellular Carcinoma

Ectopic Overexpression of COTE1 Promotes Cellular Invasion of Hepatocellular Carcinoma

Regional over-representations on chromosomes 1q, 3q and 7q in the progression of hepatitis B virus-related hepatocellular carcinoma

Identification of PFTAIRE protein kinase 1, a novel cell division cycle‐2 related gene, in the motile phenotype of hepatocellular carcinoma cells†

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