Positioning canine induced pluripotent stem cells (iPSCs) in the reprogramming landscape of naïve or primed state in comparison to mouse and human iPSCs

Menon, Dhanya V.; Bhaskar, Smitha; Sheshadri, Preethi; Joshi, Chaitanya; Patel, Darshan H.; Kumar, Anujith

doi:10.1016/j.lfs.2020.118701

Cited by 6 publications

(15 citation statements)

References 44 publications

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“…This observation suggested that LIF was not essential for ciPSC maintenance when using StemFit and iMatrix‐511, which is the same characteristic that of human iPSCs. Furthermore, a recent report has indicated that ciPSC expression of pluripotency markers is not significantly different between bFGF and LIF + bFGF conditions (Menon et al, 2021), which may be suggesting the opinion.…”

Section: Discussionmentioning

confidence: 95%

“…Since the main tissue observed in the OPUiD04‐A–injected tumors was neural tissue, it was hypothesized that the differentiation orientation was biased. In a recent report, canine iPSCs show distinct properties compared to mouse and human iPSCs and probably belong to an intermediary state of PSCs (Menon et al, 2021). Since the factors required to culture PSCs differ between humans and mice (Chen et al, 2011; Niwa et al, 2009), the optimum culture conditions for ciPSCs may be different from those of both humans and mice.…”

Section: Discussionmentioning

confidence: 99%

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Canine induced pluripotent stem cell maintenance under feeder‐free and chemically‐defined conditions

Kimura

Tsukamoto

Yoshida

et al. 2021

Molecular Reproduction Devel

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Canine induced pluripotent stem cells (ciPSCs) provide a platform for regenerative veterinary medicine, disease modeling, and drug discovery. However, in the conventional method, ciPSCs are maintained using chemically‐undefined media containing unknown animal components under on‐murine embryonic fibroblast feeder conditions, which were reported to modify cell surface of iPSCs and increases the risk of immune rejection when the cells are transplanted into patients. Moreover, in the conventional method, ciPSCs are mechanically passaged, which requires much time and effort. Therefore, the large‐scale expansion of ciPSCs is difficult, which should be resolved for using ciPSCs in clinical application and research. Here, it was shown that StemFit® AK02N and iMatrix‐511 could maintain the pluripotency of ciPSCs using conventional culture method. Furthermore, it was demonstrated that the feeder‐free and chemically‐defined ciPSC culture systems using StemFit® AK02N and iMatrix‐511 could stably maintain and allow the easy expansion of ciPSCs generated using N2B27 and StemFit® AK02N, without causing karyotype abnormalities. ciPSCs expressed several pluripotency markers and formed teratomas, including cells derived from three germ layers.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Canine induced pluripotent stem cell maintenance under feeder‐free and chemically‐defined conditions

Kimura

Tsukamoto

Yoshida

et al. 2021

Molecular Reproduction Devel

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“…Retroviral transduction Viral, genomic integrating [80,81,[88][89][90][91][92] Lentiviral transduction Viral, genomic integrating [86,93,94] Sendai viral transduction Viral, non-integrating [84,85,95,96] Synthetic RNA transfection Nonviral, non-integrating [97] Episomal plasmids Nonviral, non-integrating [98] Initial protocols followed retroviral and lentiviral transgene integrating methods and the transgenes were eventually silenced after the iPSCs were fully reprogrammed. Due to concerns over the clinical applications with evidence from numerous studies showing that viral integrating methods can induce genomic integration and increase tumorigenic potential [99,100], other non-integrating methods have been extensively studied over the last few years.…”

Section: Reprogramming Strategy Methodsmentioning

confidence: 99%

“…Both human and mouse reprogramming factors are used for iPSC derivation in animals, and in dogs it is still unknown which reprogramming factors are the best combination. Canine OSKM factors have been used to derive ciPSCs [98], but the majority of groups reportedly use human reprogramming factors [81,88,89,91]. One reprogramming factor used, c-Myc, is a proto-oncogene that can induce tumor formation, although it increases the reprogramming efficiency [102][103][104].…”

Section: Reprogramming Strategy Methodsmentioning

confidence: 99%

Genetic Basis of Dilated Cardiomyopathy in Dogs and Its Potential as a Bidirectional Model

Gaar-Humphreys

Spanjersberg

Santarelli

et al. 2022

Animals

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Cardiac disease is a leading cause of death for both humans and dogs. Genetic cardiomyopathies, including dilated cardiomyopathy (DCM), account for a proportion of these cases in both species. Patients may suffer from ventricular enlargement and systolic dysfunction resulting in congestive heart failure and ventricular arrhythmias with high risk for sudden cardiac death. Although canine DCM has similar disease progression and subtypes as in humans, only a few candidate genes have been found to be associated with DCM while the genetic background of human DCM has been more thoroughly studied. Additionally, experimental disease models using induced pluripotent stem cells have been widely adopted in the study of human genetic cardiomyopathy but have not yet been fully adapted for the in-depth study of canine genetic cardiomyopathies. The clinical presentation of DCM is extremely heterogeneous for both species with differences occurring based on sex predisposition, age of onset, and the rate of disease progression. Both genetic predisposition and environmental factors play a role in disease development which are identical in dogs and humans in contrast to other experimental animals. Interestingly, different dog breeds have been shown to develop distinct DCM phenotypes, and this presents a unique opportunity for modeling as there are multiple breed-specific models for DCM with less genetic variance than human DCM. A better understanding of DCM in dogs has the potential for improved selection for breeding and could lead to better overall care and treatment for human and canine DCM patients. At the same time, progress in research made for human DCM can have a positive impact on the care given to dogs affected by DCM. Therefore, this review will analyze the feasibility of canines as a naturally occurring bidirectional disease model for DCM in both species. The histopathology of the myocardium in canine DCM will be evaluated in three different breeds compared to control tissue, and the known genetics that contributes to both canine and human DCM will be summarized. Lastly, the prospect of canine iPSCs as a novel method to uncover the contributions of genetic variants to the pathogenesis of canine DCM will be introduced along with the applications for disease modeling and treatment.

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“…Despite its unquestionable potential in the fields of agriculture and biotechnology, iPSC research has still not received the deserved attention. Approximately thousands of studies are currently focusing on murine and human cell reprogramming, and we could find many studies that described cell reprogramming in important mammalian farm animals [ 24 , 89 , 94 , 97 , 101 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 ]. We propose that future studies should focus on the agricultural and biopharmaceutical applications of the iPSC technology.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

iPSC Technology: An Innovative Tool for Developing Clean Meat, Livestock, and Frozen Ark

Verma¹,

Lee

Salamone

2022

Animals

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Induced pluripotent stem cell (iPSC) technology is an emerging technique to reprogram somatic cells into iPSCs that have revolutionary benefits in the fields of drug discovery, cellular therapy, and personalized medicine. However, these applications are just the tip of an iceberg. Recently, iPSC technology has been shown to be useful in not only conserving the endangered species, but also the revival of extinct species. With increasing consumer reliance on animal products, combined with an ever-growing population, there is a necessity to develop alternative approaches to conventional farming practices. One such approach involves the development of domestic farm animal iPSCs. This approach provides several benefits in the form of reduced animal death, pasture degradation, water consumption, and greenhouse gas emissions. Hence, it is essentially an environmentally-friendly alternative to conventional farming. Additionally, this approach ensures decreased zoonotic outbreaks and a constant food supply. Here, we discuss the iPSC technology in the form of a “Frozen Ark”, along with its potential impact on spreading awareness of factory farming, foodborne disease, and the ecological footprint of the meat industry.

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Positioning canine induced pluripotent stem cells (iPSCs) in the reprogramming landscape of naïve or primed state in comparison to mouse and human iPSCs

Cited by 6 publications

References 44 publications

Canine induced pluripotent stem cell maintenance under feeder‐free and chemically‐defined conditions

Canine induced pluripotent stem cell maintenance under feeder‐free and chemically‐defined conditions

Genetic Basis of Dilated Cardiomyopathy in Dogs and Its Potential as a Bidirectional Model

iPSC Technology: An Innovative Tool for Developing Clean Meat, Livestock, and Frozen Ark

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