Positive allelopathic stimulation and underlying molecular mechanism of achyranthe under continuous monoculture

Abstract

Three experiments were conducted to study the allelopathic stimulation and its underlying molecular mechanism of achyranthes medicinal plants in continuously monoculture system. The stimulators in the rhizospheric soil of continuously monocultured achyranthes plants were extracted by water and organic solvents. Results of the bioassay showed that the rhizospheric soil extracts had a significant promotive effect on the growth of achyranthes in continuous monoculture system, implying that the extracts, especially the water extracts might contain plant activators to stimulate the growth of the medicinal plants. Subtractive hybridization suppression (SSH) was used to investigate gene expression profiles of achyranthes in response to the extract treatments. Ten up-regulated genes from SSH-cDNA library were sequenced and assigned. Results indicated that flavonoids and phytosterol might play an important role in the positively allelopathic stimulation on achyranthes plants in continuous monoculture system. Comparative proteomics were employed to further unveil the molecular mechanism of allelopathic stimulation induced by the extracts. Compared with protein expression profile in control, 25 differentially expressed proteins and their functions were detected and identified in the treated plants. The results suggested that the extracts from continuously monocultured rhizospheric soils under Chinese medicinal achyranthes activated the genes encoding the key enzymes involved in terpenes and flavonoids synthesis, which in turn led to increased de novo synthesis of the stimulators, and hence promoted growth of achyranthes in consecutively monoculture system.

Caesium for Perovskite Solar Cells: An Overview

“…It is reasonable thinking that the current efforts in developing novel perovskite nanostructures will be soon a precious resource in the field of sensors, electronics and energy‐related devices …”

Omics Methods for Probing the Mode of Action of Natural and Synthetic Phytotoxins

“…Li et al (2011) identified 25 differentially expressed proteins in a Chinese medicinal Achyranthes species treated with growth-promoting extracts of soil in which the same species was growing. Proteins associated with stress and secondary plant metabolism, signal transduction, synthesis and degradation of nutritive material, as well as synthesis and degradation of nucleic acids and protein were affected.…”

A Stretchable Ionic Conductive Elastomer for High‐Areal‐Capacity Lithium‐Metal Batteries

“…The rapid development of the portable electronic devices and electric vehicles places increasing demands for advanced batteries with high energy density. [ 1–4 ] However, the energy density of the current intercalation‐based lithium‐ion batteries (LIBs) is approaching to their theoretical energy density values. [ 5 ] Under this circumstance, lithium (Li)‐metal anode has been revived and attracted more and more attention [ 6–9 ] due to its high theoretical capacity (3860 mAh g −1 ) and the lowest reduction potential (−3.04 V vs standard hydrogen electrolyte).…”