Positive and Negative Regulation of Myogenic Differentiation of C2C12 Cells by Isoforms of the Multiple Homeodomain Zinc Finger Transcription Factor ATBF1

Berry, Fred B.; Miura, Yutaka; Mihara, Koichoiro; Kašpar, Petr; Sakata, Nobuo; Hashimoto-Tamaoki, Tomoko; Tamaoki, Taiki

doi:10.1074/jbc.m010378200

Cited by 73 publications

(59 citation statements)

References 63 publications

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“…Studies of ATBF1 using C2C12 cells and hepatoma cells suggested that the multiple N-terminal zinc fingers of ATBF1 play a role in muscle differentiation and alpha-fetoprotein production. 11,37) All the four homeodomains and four of the 22 zinc fingers of ZFHX4 are contained in a large exon 11, suggesting that they perform a specific role together. The position of a breakpoint of the ZFHX4 may lead the appearance of a truncated form of the protein.…”

Section: Resultsmentioning

confidence: 99%

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A Homeodomain-Zinc Finger Protein, ZFHX4, Is Expressed in Neuronal Differentiation Manner and Suppressed in Muscle Differentiation Manner

Hemmi

Miura

et al. 2006

Biological & Pharmaceutical Bulletin

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Human ZFHX4 has recently been shown to be a candidate gene for congenital bilateral isolated ptosis. Here, we report molecular cloning of the human ZFHX4 cDNA and genomic organization of this gene. Human ZFHX4 is about 180 kb long, containing 12 exons that encodes a 3599-amino acid protein carrying four homeodomains and 22 zinc fingers. The 11th exon is 3.2 kb in length and encodes all the four homeodomains together with four of the 22 zinc fingers. ZFHX4 is 90% homologous to mouse Zfhx4, 52% to human ATBF1A and 24% to Drosophila ZFH-2. ZFHX4 was mapped to human chromosome 8q13.3-q21.11 by fluorescence in situ hybridization using BAC clone RP11-48D4 as a probe. RT-PCR analysis showed that ZFHX4 transcripts were expressed in adult human brain, liver and muscle. This, together with the finding that Zfhx4 was expressed transiently in differentiating P19 embryonal carcinoma cells and C2C12 myoblasts, suggests that ZFHX4/Zfhx4 is involved in neural and muscle differentiation.

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Section: Resultsmentioning

confidence: 99%

“…9,10) ATBF1 has also been reported to be involved in the maintenance of the undifferentiated state of myoblasts. 11) Recently, various ATBF1 binding proteins have been identified which include Myb, 12) PIAS3, 13) and p53. 14) Jointly with these proteins, ATBF1 regulates various genes important for cell cycle and growth.…”

mentioning

confidence: 99%

A Homeodomain-Zinc Finger Protein, ZFHX4, Is Expressed in Neuronal Differentiation Manner and Suppressed in Muscle Differentiation Manner

Hemmi

Miura

et al. 2006

Biological & Pharmaceutical Bulletin

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“…Expression was normalized to the housekeeping gene, GAPDH, which remained constant throughout the experiment (49). The primers used for qRT-PCR to quantitate mRNA expression in C2C12 cells were as follows: Ezh2, 5Ј-GAAAAAGGACGG-CTCCTCTAA-3Ј, 3Ј-CATGGACACTGTTTGGTGTTG-5Ј; GAPDH, 5Ј-CCTGGAGAAACCTGCCAAGT-3Ј, 3Ј-AGCCG-TATTCATTGTCATACCA-5Ј; myoD, 5Ј-CATCCCTAAGC-GACACAGAAC-3Ј, 3Ј-AGCACCTGATAAATCGCATTG-5Ј; and myogenin, 5Ј-GCAGGCTCAAGAAAGTGAATG-3Ј, 3Ј-TAGGCGCTCAATGTACTGGAT-5Ј.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-26a Targets the Histone Methyltransferase Enhancer of Zeste homolog 2 during Myogenesis

Wong

Tellam

2008

Journal of Biological Chemistry

292

235

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MicroRNA (miRNA) are important regulators of many biological processes, but the targets for most miRNA are still poorly defined. In this study, we profiled the expression of miRNA during myogenesis, from proliferating myoblasts through to terminally differentiated myotubes. Microarray results identified six significantly differentially expressed miRNA that were more than 2-fold different in myotubes. From this list, miRNA-26a (miR-26a), an up-regulated miRNA, was further examined. Overexpression of miR-26a in murine myogenic C2C12 cells induced creatine kinase activity, an enzyme that markedly increases during myogenesis. Further, myoD and myogenin mRNA expression levels were also up-regulated. These results suggest that increased expression of miR-26a promotes myogenesis. Through a bioinformatics approach, we identified the histone methyltransferase, Enhancer of Zeste homolog 2 (Ezh2), as a potential target of miR-26a. Overexpression of miR-26a suppressed the activity of a luciferase reporter construct fused with the 3-untranslated region of Ezh2. In addition, miR-26a overexpression decreased Ezh2 mRNA expression. These results reveal a model of regulation during myogenesis whereby the up-regulation of miR-26a acts to post-transcriptionally repress Ezh2, a known suppressor of skeletal muscle cell differentiation.

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“…It acts primarily as a transcriptional repressor through the obstruction of E-box motifs on muscle-specific promoters. Although Zfhx3 functions as a repressor, one splice variant, Zfhx3-B, acts as a transcriptional activator that contributes to myogenic differentiation (31).…”

Section: Mef2amentioning

confidence: 99%

MEF2 Transcription Factors Regulate Distinct Gene Programs in Mammalian Skeletal Muscle Differentiation

Estrella

Desjardins

Nocco

et al. 2015

Journal of Biological Chemistry

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Background: Myocyte enhancer factor 2 (MEF2) proteins are essential for skeletal muscle development and regeneration, but their diverse roles in differentiation have not been defined. Results: Individual MEF2 proteins regulate distinct gene programs in skeletal muscle. Conclusion: Certain genes are preferentially sensitive to a specific MEF2 isoform. Significance: These findings provide opportunities to modulate MEF2 isoform-sensitive genes in skeletal muscle health and disease.

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Positive and Negative Regulation of Myogenic Differentiation of C2C12 Cells by Isoforms of the Multiple Homeodomain Zinc Finger Transcription Factor ATBF1

Abstract: These results suggest that ATBF1-A plays a role in the maintenance of the undifferentiated myoblast state, and its down-regulation is a prerequisite to initiate terminal differentiation of C2C12 cells.

Cited by 73 publications

References 63 publications

A Homeodomain-Zinc Finger Protein, ZFHX4, Is Expressed in Neuronal Differentiation Manner and Suppressed in Muscle Differentiation Manner

A Homeodomain-Zinc Finger Protein, ZFHX4, Is Expressed in Neuronal Differentiation Manner and Suppressed in Muscle Differentiation Manner

MicroRNA-26a Targets the Histone Methyltransferase Enhancer of Zeste homolog 2 during Myogenesis

MEF2 Transcription Factors Regulate Distinct Gene Programs in Mammalian Skeletal Muscle Differentiation

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