1982
DOI: 10.1128/iai.35.1.157-165.1982
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Positive Cooperativity in the Binding of Streptococcus sanguis to Hydroxylapatite

Abstract: The adherence of Streptococcus sanguis to hydroxylapatite beads has been analyzed by binding isotherms, Langmuir isotherms, and Scatchard plots. For saliva-coated beads, the Scatchard curves contained components with both positive and negative slopes. The results are interpreted as evidence for positive cooperativity in the binding process. Although all Scatchard curves were similar in shape, distinct differences were observed between saliva samples from different individuals. Salivary agglutinins against whol… Show more

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Cited by 84 publications
(56 citation statements)
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“…It seems likely, based on our observations and those reported recently by several other investigators (23,49,50) that hydrophobic interactions represent only one of the non-covalent interactions involved in the attachment of 5*. sanguis to SHA (17,37,49).…”
Section: Discussionsupporting
confidence: 81%
“…It seems likely, based on our observations and those reported recently by several other investigators (23,49,50) that hydrophobic interactions represent only one of the non-covalent interactions involved in the attachment of 5*. sanguis to SHA (17,37,49).…”
Section: Discussionsupporting
confidence: 81%
“…Binding of cells to SHA and to actinomycetes involve cell surface lectins (2,4,10,30) and lectin binding may be promoted by hydrophobic interaction (5,7). Binding of S. sanguis cells to HA is thought to involve mainly electrostatic interactions (19,32); the results presented suggest therefore that these too may be stabilized by hydrophobic forces. The mutations present in strains OB74 and OB70R in neither case appeared to affect cell surface charge.…”
Section: Discussionmentioning
confidence: 88%
“…Adsorption to hydroxylapatite beads (BDH Ltd.) or to saliva-treated hydroxylapatite (SHA) was measured using tritium-labelled cells in KCl-buffer (27) as described (32). The experiments were repeated 3 times, and the radioactive counts per minute (cpm) bound were converted to cell numbers from plots relating cpm and cfu prepared for each strain and each batch of cells.…”
Section: Adherence and Coaggregation Assaysmentioning
confidence: 99%
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